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The map label for this gene is hydG-2 [H]

Identifier: 21674073

GI number: 21674073

Start: 1177115

End: 1177951

Strand: Direct

Name: hydG-2 [H]

Synonym: CT1250

Alternate gene names: 21674073

Gene position: 1177115-1177951 (Clockwise)

Preceding gene: 21674072

Following gene: 21674074

Centisome position: 54.62

GC content: 60.57

Gene sequence:

>837_bases
ATGATCTACAGCCCGTTTCCGATGCGGGTTGTGTCAAAGCGCGCTGAAGCTCCCGGCGTCAACACCCTCAAGCTCGAATT
CGTCAAACAGGAAGATCACGAGTTTTTCAAGGCTAATTACCGGACCGGTATGTTCGGCCTCTACGGTGTCTTCGGCGAGG
GCGAAAGCACCTTCTGCGTCGCCAGCCCGGAGACCCGAAAGGAGTACATCGAATGCACCTTCCGCCAGTCGGGGCGCGTC
ACTTCGACGCTCGCCAACACCGACGCGGGCGACATCGTAACCTTCCGCGGCCCCTACGGCAACCGCTTTCCGATCGAAGA
GTTCGAGGGCAAGAACCTGCTCTTCATCGCCGGCGGCATCGCCCTGCCGCCGACGCGCAGCGTCATCTGGTCGTGCCTCG
ACCAGCGGGAGAAGTACCGCGACGTGACCATCGTTTATGGCGCGAGAACCGTAGCCGACCTCGTCTATAAAAACGAGCTC
GACGAATGGAAACAGCGCGACGACGTGCGTCTCGTGCTGACGGTCGATCCCGGAGGCGAAACCCCCGACTGGCAGGATCA
CGTCGGCTTCGTGCCGACGGTGCTCGAACAGGCCGCCCCGTCGCCGGAGAACACCATCGCCGTGCTCTGCGGCCCGCCGA
TCATGATCAAATTCACGCTAACCGCGCTCGAAAAGCTTGGCTTCACGGCGGAGAACGTTTACACCACGCTGGAGAACCGG
ATGAAATGCGGAATAGGCAAATGCGGCCGCTGCAACGTCGGTTCGATCTACATCTGCAAAGAGGGACCGGTGTTCACAGC
AGCCGAAGTGCAGGCCATGCCGCAAGCTGATCTGTAA

Upstream 100 bases:

>100_bases
GCTGCACCCGCCAGTGCCCGGTGGACATGGGTATTACTGAAACTCTGCAAGCGATTACAAATTTACCGAGGTGACACAAG
ACCGATACCATACCCACGAC

Downstream 100 bases:

>100_bases
GATATTTGAAAATGGACACAAAAAGGGGGCGGAAACGCTCCCTTTTTTTATGCAAATCATTTCTGATTCTTCATGATTTT
CCCCACCCCAACATCATTCT

Product: hydrogenase/sulfur reductase, gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIYSPFPMRVVSKRAEAPGVNTLKLEFVKQEDHEFFKANYRTGMFGLYGVFGEGESTFCVASPETRKEYIECTFRQSGRV
TSTLANTDAGDIVTFRGPYGNRFPIEEFEGKNLLFIAGGIALPPTRSVIWSCLDQREKYRDVTIVYGARTVADLVYKNEL
DEWKQRDDVRLVLTVDPGGETPDWQDHVGFVPTVLEQAAPSPENTIAVLCGPPIMIKFTLTALEKLGFTAENVYTTLENR
MKCGIGKCGRCNVGSIYICKEGPVFTAAEVQAMPQADL

Sequences:

>Translated_278_residues
MIYSPFPMRVVSKRAEAPGVNTLKLEFVKQEDHEFFKANYRTGMFGLYGVFGEGESTFCVASPETRKEYIECTFRQSGRV
TSTLANTDAGDIVTFRGPYGNRFPIEEFEGKNLLFIAGGIALPPTRSVIWSCLDQREKYRDVTIVYGARTVADLVYKNEL
DEWKQRDDVRLVLTVDPGGETPDWQDHVGFVPTVLEQAAPSPENTIAVLCGPPIMIKFTLTALEKLGFTAENVYTTLENR
MKCGIGKCGRCNVGSIYICKEGPVFTAAEVQAMPQADL
>Mature_278_residues
MIYSPFPMRVVSKRAEAPGVNTLKLEFVKQEDHEFFKANYRTGMFGLYGVFGEGESTFCVASPETRKEYIECTFRQSGRV
TSTLANTDAGDIVTFRGPYGNRFPIEEFEGKNLLFIAGGIALPPTRSVIWSCLDQREKYRDVTIVYGARTVADLVYKNEL
DEWKQRDDVRLVLTVDPGGETPDWQDHVGFVPTVLEQAAPSPENTIAVLCGPPIMIKFTLTALEKLGFTAENVYTTLENR
MKCGIGKCGRCNVGSIYICKEGPVFTAAEVQAMPQADL

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI49574502, Length=184, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17563926, Length=177, Percent_Identity=28.8135593220339, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281366101, Length=183, Percent_Identity=28.4153005464481, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24662934, Length=183, Percent_Identity=28.4153005464481, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24662938, Length=182, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI116007804, Length=183, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007806, Length=183, Percent_Identity=27.8688524590164, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: NA

Molecular weight: Translated: 30955; Mature: 30955

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYSPFPMRVVSKRAEAPGVNTLKLEFVKQEDHEFFKANYRTGMFGLYGVFGEGESTFCV
CCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCEEEE
ASPETRKEYIECTFRQSGRVTSTLANTDAGDIVTFRGPYGNRFPIEEFEGKNLLFIAGGI
ECCHHHHHHHEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHCCCCEEEEEECCC
ALPPTRSVIWSCLDQREKYRDVTIVYGARTVADLVYKNELDEWKQRDDVRLVLTVDPGGE
CCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TPDWQDHVGFVPTVLEQAAPSPENTIAVLCGPPIMIKFTLTALEKLGFTAENVYTTLENR
CCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHHCCCHHHHHHHHHHH
MKCGIGKCGRCNVGSIYICKEGPVFTAAEVQAMPQADL
HHCCCCCCCCCCCCEEEEECCCCEEEHHHHCCCCCCCC
>Mature Secondary Structure
MIYSPFPMRVVSKRAEAPGVNTLKLEFVKQEDHEFFKANYRTGMFGLYGVFGEGESTFCV
CCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCEEEE
ASPETRKEYIECTFRQSGRVTSTLANTDAGDIVTFRGPYGNRFPIEEFEGKNLLFIAGGI
ECCHHHHHHHEEEECCCCCEEEEECCCCCCCEEEEECCCCCCCCHHHCCCCEEEEEECCC
ALPPTRSVIWSCLDQREKYRDVTIVYGARTVADLVYKNELDEWKQRDDVRLVLTVDPGGE
CCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TPDWQDHVGFVPTVLEQAAPSPENTIAVLCGPPIMIKFTLTALEKLGFTAENVYTTLENR
CCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHHCCCHHHHHHHHHHH
MKCGIGKCGRCNVGSIYICKEGPVFTAAEVQAMPQADL
HHCCCCCCCCCCCCEEEEECCCCEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]