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The map label for this gene is sbcD [H]

Identifier: 21673974

GI number: 21673974

Start: 1076687

End: 1077946

Strand: Reverse

Name: sbcD [H]

Synonym: CT1148

Alternate gene names: 21673974

Gene position: 1077946-1076687 (Counterclockwise)

Preceding gene: 21673975

Following gene: 21673973

Centisome position: 50.02

GC content: 64.21

Gene sequence:

>1260_bases
TTGCCCCTACGCATTCTTCATACATCCGACTGGCATCTCGGGCGGTCGCTGTTCGGGCGGAGCCGCTTGCATGAGTTCGA
GGCGTTTCTCGACTGGCTGGCCGGAACCGTCGAGTCGCGCGGCATCGAGCTGCTCGTCGTGGCGGGCGATATTTTCGACA
CCACCACGCCCGGCAACGCCACGCAGAAACTCTACTATCAATTCCTGAACCGCATCGCTCTCACGCCCGGATCGCCATGC
CGCCACGTGGTCATCACGAGCGGCAACCACGATTCGCCCACCTTTCTCGACGCGCCGAAGGAGCTGCTTCGCGCCTTCGA
CGTGCATGTGCTCGGCGCGACGACCGGTGATCCGGCGGACGAGGTGCGGCTGCTCCGCGATCGGCAGGGAAGTCCCGAGG
CGATTGTGTGCGCGGTGCCGTTCTTGCGGGATCGCGACATTCGCACGGTCGAGCCGGGGGAGAGTCTGGAGGACAAAATC
CGCAAGCTCTCCGACGGCGTGTCGCTGCACTACGCCGAGGTGGCGCGGATCGCCGCTGAACGCCGTCGGAGTCTCGGTGC
GGATATTCCGGTCATCGCGATGGGGCACCTCTTTACGGCGGGGTGTGTCACGGTTGAGGAGGATGGCGTGCGTGAAATTT
ACGCCGGAGCGCTCTCGATTGTCTGCTCGACCGCCTTTCCGCCTGTTTTCGACTACGTCGCCCTCGGCCACATGCACGTG
CCGCAACGGGTCGATAAAACGGAACATATGCGCTACTGCGGCTCGCCGATTCCGATGGGATTTGGCGAGGCGAAGCAGCA
AAAGCTGGTGCTTCAGGTTGATTTCGAAGGGCGCGAGCCAACCGTCACGGAGATCGAAGTTCCCTGTTTCCAGCCGCTCG
AACGACTCGCCGGGAGCCTCGACGAACTGAGCGCCGCCATCGCTGCGTTGCGGTCGGCGGGCAGCAACGCCTGGCTGGAG
ATCGACTATCGCGGAGACGAAGCGCCCGCAACCGTGCAGGAGGCGATGGAACAGGCGGTCGCGGGATCGCTGCTCGAAAT
CCGGCGCATCCGCAACAACCGCCCGCTCCAGCAGGCGCTGCGACACTCCGCAGCGAACGAGACGCTCCAGCAGCTCGATC
CCGAAGCGGTCTTCAACCGCTGCCTCGAAGCGTACGGCACCGACGAATCGCTCCGTCCCGCGCTCGTCGAGAGCTACCGC
GAAGTGCTGCGCTCAATCCGCGAGGAGGACGTGATGGCCGAAAAGGAGGGGAAATCGTGA

Upstream 100 bases:

>100_bases
TTTGTCATAAAGACACCAGTTTTTGTTTTATCGCCGCTTGGCGCTCCCGGCGTCGCTGCCTACATTGTGCATTATCCATT
ATCAATTTTTCCATTATCTC

Downstream 100 bases:

>100_bases
TTATCCAGAAGCTTCGTTTCGCCAATCTGAATTCGTTGCAAGGGGAGTGGGAGATCGATTTCACCCGGCCCGAATATCTT
TCGGACGGCCTCTTCGCCAT

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPLRILHTSDWHLGRSLFGRSRLHEFEAFLDWLAGTVESRGIELLVVAGDIFDTTTPGNATQKLYYQFLNRIALTPGSPC
RHVVITSGNHDSPTFLDAPKELLRAFDVHVLGATTGDPADEVRLLRDRQGSPEAIVCAVPFLRDRDIRTVEPGESLEDKI
RKLSDGVSLHYAEVARIAAERRRSLGADIPVIAMGHLFTAGCVTVEEDGVREIYAGALSIVCSTAFPPVFDYVALGHMHV
PQRVDKTEHMRYCGSPIPMGFGEAKQQKLVLQVDFEGREPTVTEIEVPCFQPLERLAGSLDELSAAIAALRSAGSNAWLE
IDYRGDEAPATVQEAMEQAVAGSLLEIRRIRNNRPLQQALRHSAANETLQQLDPEAVFNRCLEAYGTDESLRPALVESYR
EVLRSIREEDVMAEKEGKS

Sequences:

>Translated_419_residues
MPLRILHTSDWHLGRSLFGRSRLHEFEAFLDWLAGTVESRGIELLVVAGDIFDTTTPGNATQKLYYQFLNRIALTPGSPC
RHVVITSGNHDSPTFLDAPKELLRAFDVHVLGATTGDPADEVRLLRDRQGSPEAIVCAVPFLRDRDIRTVEPGESLEDKI
RKLSDGVSLHYAEVARIAAERRRSLGADIPVIAMGHLFTAGCVTVEEDGVREIYAGALSIVCSTAFPPVFDYVALGHMHV
PQRVDKTEHMRYCGSPIPMGFGEAKQQKLVLQVDFEGREPTVTEIEVPCFQPLERLAGSLDELSAAIAALRSAGSNAWLE
IDYRGDEAPATVQEAMEQAVAGSLLEIRRIRNNRPLQQALRHSAANETLQQLDPEAVFNRCLEAYGTDESLRPALVESYR
EVLRSIREEDVMAEKEGKS
>Mature_418_residues
PLRILHTSDWHLGRSLFGRSRLHEFEAFLDWLAGTVESRGIELLVVAGDIFDTTTPGNATQKLYYQFLNRIALTPGSPCR
HVVITSGNHDSPTFLDAPKELLRAFDVHVLGATTGDPADEVRLLRDRQGSPEAIVCAVPFLRDRDIRTVEPGESLEDKIR
KLSDGVSLHYAEVARIAAERRRSLGADIPVIAMGHLFTAGCVTVEEDGVREIYAGALSIVCSTAFPPVFDYVALGHMHVP
QRVDKTEHMRYCGSPIPMGFGEAKQQKLVLQVDFEGREPTVTEIEVPCFQPLERLAGSLDELSAAIAALRSAGSNAWLEI
DYRGDEAPATVQEAMEQAVAGSLLEIRRIRNNRPLQQALRHSAANETLQQLDPEAVFNRCLEAYGTDESLRPALVESYRE
VLRSIREEDVMAEKEGKS

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=415, Percent_Identity=35.421686746988, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46394; Mature: 46263

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRILHTSDWHLGRSLFGRSRLHEFEAFLDWLAGTVESRGIELLVVAGDIFDTTTPGNA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCH
TQKLYYQFLNRIALTPGSPCRHVVITSGNHDSPTFLDAPKELLRAFDVHVLGATTGDPAD
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHH
EVRLLRDRQGSPEAIVCAVPFLRDRDIRTVEPGESLEDKIRKLSDGVSLHYAEVARIAAE
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
RRRSLGADIPVIAMGHLFTAGCVTVEEDGVREIYAGALSIVCSTAFPPVFDYVALGHMHV
HHHHCCCCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PQRVDKTEHMRYCGSPIPMGFGEAKQQKLVLQVDFEGREPTVTEIEVPCFQPLERLAGSL
CHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHHHCCH
DELSAAIAALRSAGSNAWLEIDYRGDEAPATVQEAMEQAVAGSLLEIRRIRNNRPLQQAL
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RHSAANETLQQLDPEAVFNRCLEAYGTDESLRPALVESYREVLRSIREEDVMAEKEGKS
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLRILHTSDWHLGRSLFGRSRLHEFEAFLDWLAGTVESRGIELLVVAGDIFDTTTPGNA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCH
TQKLYYQFLNRIALTPGSPCRHVVITSGNHDSPTFLDAPKELLRAFDVHVLGATTGDPAD
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHH
EVRLLRDRQGSPEAIVCAVPFLRDRDIRTVEPGESLEDKIRKLSDGVSLHYAEVARIAAE
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
RRRSLGADIPVIAMGHLFTAGCVTVEEDGVREIYAGALSIVCSTAFPPVFDYVALGHMHV
HHHHCCCCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PQRVDKTEHMRYCGSPIPMGFGEAKQQKLVLQVDFEGREPTVTEIEVPCFQPLERLAGSL
CHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCHHHHHHHHHCCH
DELSAAIAALRSAGSNAWLEIDYRGDEAPATVQEAMEQAVAGSLLEIRRIRNNRPLQQAL
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RHSAANETLQQLDPEAVFNRCLEAYGTDESLRPALVESYREVLRSIREEDVMAEKEGKS
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]