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The map label for this gene is dfrA [H]

Identifier: 21673863

GI number: 21673863

Start: 974550

End: 975545

Strand: Direct

Name: dfrA [H]

Synonym: CT1037

Alternate gene names: 21673863

Gene position: 974550-975545 (Clockwise)

Preceding gene: 21673862

Following gene: 21673864

Centisome position: 45.22

GC content: 52.71

Gene sequence:

>996_bases
GTGAGAAAGAAAATAGTCGTAACCGGAGGCACCGGGTTCATCGGGTCCCGTCTTGTCCACAGGCTGGCTGCGTCTGGCGA
AGATGTTTACGTGCTGGTCAGGGCTAGCTCCGATTTAGCCTCTCTCAAGGAGTGCCTTGACCGGATTACTCTCGTTTACG
GCGATGTGACGGATATCGCTTCGCTTTCCGGAGCGTTTGAGGGAGCTGAAGAGGTCTATCATTGCGCAGGTATCACCTAT
ATGGGCGACCGTAAAAATCCCCTGTTGCAGAGAATCAATGTCGAAGGCACACAGAACGTGCTCGATGCCTGTCGCCGGGC
AAAGGTCAAAAGGGTGGTGCATGTGAGTTCCATCACGGCGGTGGGAATCAGCGGTCCGAACCGCAAGTTCAACGAGGAGA
GCTGCTGGAATTTCGACACTATCGATCTGGAGTATGCCAGGACCAAACATGCTGCGGAAAAGATCGTCGCCGCAGCGGTG
AAAAAAGGTATGGACTGTGTCATCGTGGTGCCGGCATTTGTCTTCGGTGCGGGTGATATCAATTTTAACGCCGGGCGCAT
CATCAAGGATGTCTACAAGCGCAAGATGCCGTTTTATCCATTGGGAGGAATATGCGTAGTGGATGTGGAGATCGTGGTTG
ACTGTCTCATTGCAGCCATGAAGAAAGGGCGCACGGGCGAGCGCTATATTGTCGGCGGCGACAATGTTTCGTTCAAAGAA
TTGGCCCAGACGATCATGGATGTCACCGGCGTGCACCAGCGTTCCTTTCCGCTGCCGATCTGGGCAGCTCATGCGGTCAG
TTTTCTCCTTAAGTTCAGTCCGGAAAGGAAAAAGATTTCGAAGCTGTTCAACATGACCATGTTCACCGTGGCCTCGAAAT
TTTTGTATTTCGATTCGTCGAAAGCCCAGCGGGAATTGGGTATGCGCTATGAACCGTTTGCAGAGAGCATCCGGCGGACG
TTTGAATGGTATCGTGAACGCCGTATGCTGAACTGA

Upstream 100 bases:

>100_bases
TTCAGGAGTAAAAAGTCATCATAAGACGGATATTGTTCCGGAGATCGAATCCGGGGCGTCATTCGTCAAAGTACCAGCTC
TTCAATACCATAGTGCATTT

Downstream 100 bases:

>100_bases
CGCCGCGTCTCATCTGGGAGCAAGTATTGTTTTATGCTGGTGCCTGCCTGTAGCCGGTCAAAAGCCTTCAACACCTCAGT
CATCGAGAGGAGGATTGTCC

Product: 3-beta hydroxysteroid dehydrogenase/isomerase family protein

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRKKIVVTGGTGFIGSRLVHRLAASGEDVYVLVRASSDLASLKECLDRITLVYGDVTDIASLSGAFEGAEEVYHCAGITY
MGDRKNPLLQRINVEGTQNVLDACRRAKVKRVVHVSSITAVGISGPNRKFNEESCWNFDTIDLEYARTKHAAEKIVAAAV
KKGMDCVIVVPAFVFGAGDINFNAGRIIKDVYKRKMPFYPLGGICVVDVEIVVDCLIAAMKKGRTGERYIVGGDNVSFKE
LAQTIMDVTGVHQRSFPLPIWAAHAVSFLLKFSPERKKISKLFNMTMFTVASKFLYFDSSKAQRELGMRYEPFAESIRRT
FEWYRERRMLN

Sequences:

>Translated_331_residues
MRKKIVVTGGTGFIGSRLVHRLAASGEDVYVLVRASSDLASLKECLDRITLVYGDVTDIASLSGAFEGAEEVYHCAGITY
MGDRKNPLLQRINVEGTQNVLDACRRAKVKRVVHVSSITAVGISGPNRKFNEESCWNFDTIDLEYARTKHAAEKIVAAAV
KKGMDCVIVVPAFVFGAGDINFNAGRIIKDVYKRKMPFYPLGGICVVDVEIVVDCLIAAMKKGRTGERYIVGGDNVSFKE
LAQTIMDVTGVHQRSFPLPIWAAHAVSFLLKFSPERKKISKLFNMTMFTVASKFLYFDSSKAQRELGMRYEPFAESIRRT
FEWYRERRMLN
>Mature_331_residues
MRKKIVVTGGTGFIGSRLVHRLAASGEDVYVLVRASSDLASLKECLDRITLVYGDVTDIASLSGAFEGAEEVYHCAGITY
MGDRKNPLLQRINVEGTQNVLDACRRAKVKRVVHVSSITAVGISGPNRKFNEESCWNFDTIDLEYARTKHAAEKIVAAAV
KKGMDCVIVVPAFVFGAGDINFNAGRIIKDVYKRKMPFYPLGGICVVDVEIVVDCLIAAMKKGRTGERYIVGGDNVSFKE
LAQTIMDVTGVHQRSFPLPIWAAHAVSFLLKFSPERKKISKLFNMTMFTVASKFLYFDSSKAQRELGMRYEPFAESIRRT
FEWYRERRMLN

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=355, Percent_Identity=25.3521126760563, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI193211614, Length=347, Percent_Identity=23.0547550432277, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI8393516, Length=347, Percent_Identity=23.0547550432277, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310132178, Length=283, Percent_Identity=26.8551236749117, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310113012, Length=283, Percent_Identity=26.8551236749117, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239745448, Length=283, Percent_Identity=26.8551236749117, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504507, Length=369, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321437, Length=361, Percent_Identity=24.9307479224377, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37005; Mature: 37005

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIVVTGGTGFIGSRLVHRLAASGEDVYVLVRASSDLASLKECLDRITLVYGDVTDIA
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
SLSGAFEGAEEVYHCAGITYMGDRKNPLLQRINVEGTQNVLDACRRAKVKRVVHVSSITA
HHHHHHCCHHHHHHHHCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
VGISGPNRKFNEESCWNFDTIDLEYARTKHAAEKIVAAAVKKGMDCVIVVPAFVFGAGDI
EECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCC
NFNAGRIIKDVYKRKMPFYPLGGICVVDVEIVVDCLIAAMKKGRTGERYIVGGDNVSFKE
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LAQTIMDVTGVHQRSFPLPIWAAHAVSFLLKFSPERKKISKLFNMTMFTVASKFLYFDSS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KAQRELGMRYEPFAESIRRTFEWYRERRMLN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKKIVVTGGTGFIGSRLVHRLAASGEDVYVLVRASSDLASLKECLDRITLVYGDVTDIA
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
SLSGAFEGAEEVYHCAGITYMGDRKNPLLQRINVEGTQNVLDACRRAKVKRVVHVSSITA
HHHHHHCCHHHHHHHHCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEE
VGISGPNRKFNEESCWNFDTIDLEYARTKHAAEKIVAAAVKKGMDCVIVVPAFVFGAGDI
EECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCC
NFNAGRIIKDVYKRKMPFYPLGGICVVDVEIVVDCLIAAMKKGRTGERYIVGGDNVSFKE
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LAQTIMDVTGVHQRSFPLPIWAAHAVSFLLKFSPERKKISKLFNMTMFTVASKFLYFDSS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KAQRELGMRYEPFAESIRRTFEWYRERRMLN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]