Click here to switch to the map view.

The map label for this gene is rsmD [H]

Identifier: 21673792

GI number: 21673792

Start: 909010

End: 909564

Strand: Direct

Name: rsmD [H]

Synonym: CT0964

Alternate gene names: 21673792

Gene position: 909010-909564 (Clockwise)

Preceding gene: 21673791

Following gene: 21673793

Centisome position: 42.18

GC content: 57.84

Gene sequence:

>555_bases
TTGCAGATTCATGCGGGCAGATACCGGGGGCGAAAGATCAGGCATCTGCCTTCGCGCGACATCCGGCCCTGCACCAGCAG
GGTCAAGAAGTCGCTTTTCGACACACTTGCAGCGCGTCTCGATTTCGATGACATCGAGGTGCTCGATCTTTTCGCCGGCT
TCGGCAATCTCGGCTTTGAGGCGCTGAGCCGGGGTGCCAAGAGCGCCTGCTTCGTGGAGCAGAACCGGCAGGCGCTCGAA
ACGATGAAGGCCACAGCGGTGGACATTGGCGCGGGTGCTTCGGCGCGGTTTGTCATGGCTGACGTTACGGCGTTTCTGAA
AAGGGAAGAGGGGTCGTTCGATCTGGTGTTCTGCGATCCGCCCTATCGCTGGGAGGATTACGAGTATCTGATCCGCTCGA
TTCTCGATACCGGCCTGCTTGCCCCGGATGGCCTGCTTCTCATGGAGCATCACGCGAGTCATGATTTTTCGCAATCTCGC
GGGTATCTCTTCCACAAGGATTACGGCACGACCCGAGTCTCGTTTTTTACACCACAACCTGAAGCGCATGAGTAA

Upstream 100 bases:

>100_bases
AACTGGCATGGCAAACCGCCATCCAAAGAGGACGAGGCAAAAGCGCTGGAGATTCTCGGCGTAACTATTTTCGGTGACTT
TTAAGCTGTTTCGATAGGTT

Downstream 100 bases:

>100_bases
GAAAGCCATCTATCCGGGGACCTTCGATCCCTTCACCAACGGCCATCTCGATGTGCTCGAGCGCGCGCTGAACATTTTTG
AACATGTTGACGTGGTGCTT

Product: methylase, putative

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MQIHAGRYRGRKIRHLPSRDIRPCTSRVKKSLFDTLAARLDFDDIEVLDLFAGFGNLGFEALSRGAKSACFVEQNRQALE
TMKATAVDIGAGASARFVMADVTAFLKREEGSFDLVFCDPPYRWEDYEYLIRSILDTGLLAPDGLLLMEHHASHDFSQSR
GYLFHKDYGTTRVSFFTPQPEAHE

Sequences:

>Translated_184_residues
MQIHAGRYRGRKIRHLPSRDIRPCTSRVKKSLFDTLAARLDFDDIEVLDLFAGFGNLGFEALSRGAKSACFVEQNRQALE
TMKATAVDIGAGASARFVMADVTAFLKREEGSFDLVFCDPPYRWEDYEYLIRSILDTGLLAPDGLLLMEHHASHDFSQSR
GYLFHKDYGTTRVSFFTPQPEAHE
>Mature_184_residues
MQIHAGRYRGRKIRHLPSRDIRPCTSRVKKSLFDTLAARLDFDDIEVLDLFAGFGNLGFEALSRGAKSACFVEQNRQALE
TMKATAVDIGAGASARFVMADVTAFLKREEGSFDLVFCDPPYRWEDYEYLIRSILDTGLLAPDGLLLMEHHASHDFSQSR
GYLFHKDYGTTRVSFFTPQPEAHE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=184, Percent_Identity=32.6086956521739, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20841; Mature: 20841

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHAGRYRGRKIRHLPSRDIRPCTSRVKKSLFDTLAARLDFDDIEVLDLFAGFGNLGFE
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHH
ALSRGAKSACFVEQNRQALETMKATAVDIGAGASARFVMADVTAFLKREEGSFDLVFCDP
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
PYRWEDYEYLIRSILDTGLLAPDGLLLMEHHASHDFSQSRGYLFHKDYGTTRVSFFTPQP
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCCEEEEECCCCEEEEEECCCC
EAHE
CCCC
>Mature Secondary Structure
MQIHAGRYRGRKIRHLPSRDIRPCTSRVKKSLFDTLAARLDFDDIEVLDLFAGFGNLGFE
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHH
ALSRGAKSACFVEQNRQALETMKATAVDIGAGASARFVMADVTAFLKREEGSFDLVFCDP
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
PYRWEDYEYLIRSILDTGLLAPDGLLLMEHHASHDFSQSRGYLFHKDYGTTRVSFFTPQP
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCCEEEEECCCCEEEEEECCCC
EAHE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]