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The map label for this gene is adh1 [H]

Identifier: 21673779

GI number: 21673779

Start: 897825

End: 898976

Strand: Direct

Name: adh1 [H]

Synonym: CT0951

Alternate gene names: 21673779

Gene position: 897825-898976 (Clockwise)

Preceding gene: 21673778

Following gene: 21673781

Centisome position: 41.66

GC content: 66.84

Gene sequence:

>1152_bases
ATGCCGCGCATCCACTTCGGGCCGGGTGCGCTCGCGAAGCTTCCGGCACTTGCCGCCGCGTTTGGGCGGCGGATGCTGCT
TGTAACGGGGCGGCAGACGCTGCGGCGCTCGCCGGTTGCGTCGCGAATGCTCAACGAACTGCGCCACGCCGGGATGGAGG
TTGCTTGCCTCGAAGTCGAGCGCGAGCCGTCGCCGGAGCTGATCAACGAGGCTGCGGCGCTCGGTCGCCAGAAGCCGTTC
GAGGTCGTTGTCGCCATTGGCGGCGGCAGCGCCTTGGATGCCGGAAAGGCGATCTCGGCGATGCTCTTGCAGCGCGATCC
GGTCGAGCGCTTCATCGAGGATCAGCCCGGCTTCACGCCGCACGACGGTCGCAAGGTTCCGTTTATCGCCGTGCCGACCA
CCTCTGGCACCGGCACCGAAGCGACGAGCAACGCCGTCATCAGCCGCATCGGGCCGGGTGGCTTCAAGCGCTCGCTGCGC
CATCCGGCATTCGTGCCCGATGTGGCGATCATCGATCCAGAGCTGATGGTGAGCTTGCCGCGCGAGGTGACGGTCTCAAC
CGGCATGGACGCTTTCACACAGTTGCTCGAAGCCTACCTCTCGCCCTTCGCCAGCGCCTACACCGATGCGCTCTGTTGCA
GCGGCCTGGAGCACTTCGCCCGCTCGTTCGCAGTTGCCAGCGGCGACGGCGCGGCTTCGGTTGCCGTGCGCGCCGACATG
GCCTACGCGGCGCTGATGTCTGGCATCGTGCTGGCCAACGCGGGCCTCGGCATCGTGCACGGCTTCGCTTCGTCGGTCGG
CGGCCTGTTCGAGATTCCGCATGGCACGCTCTGCGCAACGCTGCTCGCCGAATCGACGCGCGAGAACATTCGCCAGCTCA
GAGCCTCGGAGGGCGGCGAGCTGGGCTTGCACAAGTTCGCCAACGCCAGCCGCATCCTCACCGGGAGCGCGACAGGCAAC
CTCGCTGCCGACTGTGACCGCCTCGTCGAGCTGCTCGAAAGGTGGCAGGAGCGCTTCGCCTTCCCACGCCTCGGCGAGTT
CGGCGTGCGCGAGAGCGATTTCGCGGAGATTATTGCTGCCACCCGGAGCAAGAGCAACGCTGTTCCGCTCGATGCTTCGG
CAATGCAGCGGATTTTAGCGGCGAGGGTTTGA

Upstream 100 bases:

>100_bases
GTACCCTGCCGGACGGCCAACTGCCCGCACTGCGGCAAAATGCTGATTCGCAAGGGGAGGTACAGCCGTGTCGATTGAGA
TGCAGTTTTCGGTGCTGCCG

Downstream 100 bases:

>100_bases
TGAGTTGATAATGGATAGTTGACAGTTGATAGTTTGGGAAGAGCAAAGTCCGAACCCCGAAAGGCGTTTTGGCGGCCACC
CTTGATTTGTTGCGCTCAAG

Product: alcohol dehydrogenase, iron-containing

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPRIHFGPGALAKLPALAAAFGRRMLLVTGRQTLRRSPVASRMLNELRHAGMEVACLEVEREPSPELINEAAALGRQKPF
EVVVAIGGGSALDAGKAISAMLLQRDPVERFIEDQPGFTPHDGRKVPFIAVPTTSGTGTEATSNAVISRIGPGGFKRSLR
HPAFVPDVAIIDPELMVSLPREVTVSTGMDAFTQLLEAYLSPFASAYTDALCCSGLEHFARSFAVASGDGAASVAVRADM
AYAALMSGIVLANAGLGIVHGFASSVGGLFEIPHGTLCATLLAESTRENIRQLRASEGGELGLHKFANASRILTGSATGN
LAADCDRLVELLERWQERFAFPRLGEFGVRESDFAEIIAATRSKSNAVPLDASAMQRILAARV

Sequences:

>Translated_383_residues
MPRIHFGPGALAKLPALAAAFGRRMLLVTGRQTLRRSPVASRMLNELRHAGMEVACLEVEREPSPELINEAAALGRQKPF
EVVVAIGGGSALDAGKAISAMLLQRDPVERFIEDQPGFTPHDGRKVPFIAVPTTSGTGTEATSNAVISRIGPGGFKRSLR
HPAFVPDVAIIDPELMVSLPREVTVSTGMDAFTQLLEAYLSPFASAYTDALCCSGLEHFARSFAVASGDGAASVAVRADM
AYAALMSGIVLANAGLGIVHGFASSVGGLFEIPHGTLCATLLAESTRENIRQLRASEGGELGLHKFANASRILTGSATGN
LAADCDRLVELLERWQERFAFPRLGEFGVRESDFAEIIAATRSKSNAVPLDASAMQRILAARV
>Mature_382_residues
PRIHFGPGALAKLPALAAAFGRRMLLVTGRQTLRRSPVASRMLNELRHAGMEVACLEVEREPSPELINEAAALGRQKPFE
VVVAIGGGSALDAGKAISAMLLQRDPVERFIEDQPGFTPHDGRKVPFIAVPTTSGTGTEATSNAVISRIGPGGFKRSLRH
PAFVPDVAIIDPELMVSLPREVTVSTGMDAFTQLLEAYLSPFASAYTDALCCSGLEHFARSFAVASGDGAASVAVRADMA
YAALMSGIVLANAGLGIVHGFASSVGGLFEIPHGTLCATLLAESTRENIRQLRASEGGELGLHKFANASRILTGSATGNL
AADCDRLVELLERWQERFAFPRLGEFGVRESDFAEIIAATRSKSNAVPLDASAMQRILAARV

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=350, Percent_Identity=32, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082107, Length=282, Percent_Identity=32.6241134751773, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789163, Length=353, Percent_Identity=29.4617563739377, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI48994951, Length=335, Percent_Identity=30.1492537313433, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789386, Length=206, Percent_Identity=30.0970873786408, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=287, Percent_Identity=25.4355400696864, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321181, Length=362, Percent_Identity=31.4917127071823, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24657991, Length=293, Percent_Identity=28.6689419795222, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40547; Mature: 40416

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIHFGPGALAKLPALAAAFGRRMLLVTGRQTLRRSPVASRMLNELRHAGMEVACLEVE
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHCCHHHHHHHHHHHHCCCEEEEEEEC
REPSPELINEAAALGRQKPFEVVVAIGGGSALDAGKAISAMLLQRDPVERFIEDQPGFTP
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
HDGRKVPFIAVPTTSGTGTEATSNAVISRIGPGGFKRSLRHPAFVPDVAIIDPELMVSLP
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECHHHHHHCC
REVTVSTGMDAFTQLLEAYLSPFASAYTDALCCSGLEHFARSFAVASGDGAASVAVRADM
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
AYAALMSGIVLANAGLGIVHGFASSVGGLFEIPHGTLCATLLAESTRENIRQLRASEGGE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LGLHKFANASRILTGSATGNLAADCDRLVELLERWQERFAFPRLGEFGVRESDFAEIIAA
CCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
TRSKSNAVPLDASAMQRILAARV
HCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PRIHFGPGALAKLPALAAAFGRRMLLVTGRQTLRRSPVASRMLNELRHAGMEVACLEVE
CCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHCCHHHHHHHHHHHHCCCEEEEEEEC
REPSPELINEAAALGRQKPFEVVVAIGGGSALDAGKAISAMLLQRDPVERFIEDQPGFTP
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
HDGRKVPFIAVPTTSGTGTEATSNAVISRIGPGGFKRSLRHPAFVPDVAIIDPELMVSLP
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECHHHHHHCC
REVTVSTGMDAFTQLLEAYLSPFASAYTDALCCSGLEHFARSFAVASGDGAASVAVRADM
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
AYAALMSGIVLANAGLGIVHGFASSVGGLFEIPHGTLCATLLAESTRENIRQLRASEGGE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LGLHKFANASRILTGSATGNLAADCDRLVELLERWQERFAFPRLGEFGVRESDFAEIIAA
CCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
TRSKSNAVPLDASAMQRILAARV
HCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]