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The map label for this gene is spoU [H]

Identifier: 21673707

GI number: 21673707

Start: 833971

End: 834660

Strand: Reverse

Name: spoU [H]

Synonym: CT0879

Alternate gene names: 21673707

Gene position: 834660-833971 (Counterclockwise)

Preceding gene: 21673709

Following gene: 21673705

Centisome position: 38.73

GC content: 58.55

Gene sequence:

>690_bases
ATGCCTCAAGCACGGCTTCGTCAAACAAACCTCATTGCATTGGTCTCACCTGAACGATTCCGGAAAATCCGTGAGCTGCT
CGAGAAGCGGCAAACCGACCTGACACTGGTCATGGACAACGTCAACAAGCCGCACAACCTGGCCGCCATCATCCGAAGCT
GCGACGCAGTCGGCATTCACCAGGTGCACGCTATCTCCTACCGCTCCTCGATCAAGACCAAGCAGCACACAGCAGCGGGC
GCAAGCCGCTGGCTCAAGGTACACCTCAACGAATCGTTTGTACCGGTCGGCGAGAAGCTCCGGCAGTCGGGAATGCAGAT
TCTCGTAGCCCACTACTGCCCGGAGGCGGTCAATTTCCGCTCTATAGACTACACCCGCCCAACAGCCATCGTCATGGGCG
AAGAGCTGAAAGGGCCATCGCAGGAGGCGCTCGGCCTCGCCGACAACTTCATCTACATCCCGATGATGGGCATGGTCGAG
TCGCTGAACGTCTCGGTCGCCGCCGCGCTCATTCTTTTCGAAGCACAACGCCAGCGGCAGGCCGCCGGATTGTACGACAC
ACGCCACTTCAGCGACTCCGACATCGAGCGTCTGCTCTTTGAGTACACCTATCCACGCCTTGCCGCCGTGCTCCGCGATC
AAGGTAAACCATACCCGAAACTCAACGAAAACGGCGCATTCACGGAGTGA

Upstream 100 bases:

>100_bases
GAATATACTTTTAAGAATTGCATTTACCTCGTTTTGCAAAGGATCTGCCATTCATGGATGCCTGGTTGAAGATTTAACGA
AAAATGACTTTCTTTTCAGA

Downstream 100 bases:

>100_bases
GGCAAATGCCCCCCGTGGTATTTCGCTGAAATATTTTTTCAAAAACCTGACCATATACATAAAACACACAAACAAAGCCC
CTCAAAAACCTATAACGTTA

Product: tRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPQARLRQTNLIALVSPERFRKIRELLEKRQTDLTLVMDNVNKPHNLAAIIRSCDAVGIHQVHAISYRSSIKTKQHTAAG
ASRWLKVHLNESFVPVGEKLRQSGMQILVAHYCPEAVNFRSIDYTRPTAIVMGEELKGPSQEALGLADNFIYIPMMGMVE
SLNVSVAAALILFEAQRQRQAAGLYDTRHFSDSDIERLLFEYTYPRLAAVLRDQGKPYPKLNENGAFTE

Sequences:

>Translated_229_residues
MPQARLRQTNLIALVSPERFRKIRELLEKRQTDLTLVMDNVNKPHNLAAIIRSCDAVGIHQVHAISYRSSIKTKQHTAAG
ASRWLKVHLNESFVPVGEKLRQSGMQILVAHYCPEAVNFRSIDYTRPTAIVMGEELKGPSQEALGLADNFIYIPMMGMVE
SLNVSVAAALILFEAQRQRQAAGLYDTRHFSDSDIERLLFEYTYPRLAAVLRDQGKPYPKLNENGAFTE
>Mature_228_residues
PQARLRQTNLIALVSPERFRKIRELLEKRQTDLTLVMDNVNKPHNLAAIIRSCDAVGIHQVHAISYRSSIKTKQHTAAGA
SRWLKVHLNESFVPVGEKLRQSGMQILVAHYCPEAVNFRSIDYTRPTAIVMGEELKGPSQEALGLADNFIYIPMMGMVES
LNVSVAAALILFEAQRQRQAAGLYDTRHFSDSDIERLLFEYTYPRLAAVLRDQGKPYPKLNENGAFTE

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=213, Percent_Identity=51.1737089201878, Blast_Score=223, Evalue=7e-60,
Organism=Escherichia coli, GI1790623, Length=175, Percent_Identity=33.1428571428571, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25864; Mature: 25733

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQARLRQTNLIALVSPERFRKIRELLEKRQTDLTLVMDNVNKPHNLAAIIRSCDAVGIH
CCCCHHCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCHH
QVHAISYRSSIKTKQHTAAGASRWLKVHLNESFVPVGEKLRQSGMQILVAHYCPEAVNFR
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCHHEEHHHHCCCCCCCC
SIDYTRPTAIVMGEELKGPSQEALGLADNFIYIPMMGMVESLNVSVAAALILFEAQRQRQ
CCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGLYDTRHFSDSDIERLLFEYTYPRLAAVLRDQGKPYPKLNENGAFTE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQARLRQTNLIALVSPERFRKIRELLEKRQTDLTLVMDNVNKPHNLAAIIRSCDAVGIH
CCCHHCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCHH
QVHAISYRSSIKTKQHTAAGASRWLKVHLNESFVPVGEKLRQSGMQILVAHYCPEAVNFR
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCHHEEHHHHCCCCCCCC
SIDYTRPTAIVMGEELKGPSQEALGLADNFIYIPMMGMVESLNVSVAAALILFEAQRQRQ
CCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGLYDTRHFSDSDIERLLFEYTYPRLAAVLRDQGKPYPKLNENGAFTE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]