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The map label for this gene is dsrH [H]

Identifier: 21673686

GI number: 21673686

Start: 816351

End: 816647

Strand: Direct

Name: dsrH [H]

Synonym: CT0857

Alternate gene names: 21673686

Gene position: 816351-816647 (Clockwise)

Preceding gene: 21673685

Following gene: 21673688

Centisome position: 37.88

GC content: 55.22

Gene sequence:

>297_bases
ATGTTACATACCATCAATAAATCTCCGTTTGAAAACAACACCTTCACTACCTGCGTCCGGTTCCTTCAGCCGGGAGACCC
CGTGCTCTTCATCGAGGACGGCGTCTATGCGGTACAGGCAGGTAACCGGTTCGGCGCGCTGATCCAGTCCGTCCTGAAGA
AGAACAATCCAGTCTATGCCCTCAAGCCCGATCTCGACGCTCGCGGTATTTCCGCTATCGCCGAGGGGGTGAAGACGGTC
GATTATGCCGGTTTCGTCGATCTGGTCGAAGAGCACCAGGTCAACAGCTGGCTGTAA

Upstream 100 bases:

>100_bases
CGCCTGATGAACGAACAGGATGTCGTCATTCACCATTGAACCGGCCCGCGATTTCCTGGGCTGAATAACGAGCAAAGAAC
ACAGAAACGTAACTTACATC

Downstream 100 bases:

>100_bases
TCTTTTCGGAACATTGCTGTTCTCCCGGTTTGCCGACGGGATATTATTTCTGTTGGCGAACCGGTATTAAAGTGGCGCGA
TTTGCCGGTGCGGTTTTAGC

Product: DsrH protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B [H]

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MLHTINKSPFENNTFTTCVRFLQPGDPVLFIEDGVYAVQAGNRFGALIQSVLKKNNPVYALKPDLDARGISAIAEGVKTV
DYAGFVDLVEEHQVNSWL

Sequences:

>Translated_98_residues
MLHTINKSPFENNTFTTCVRFLQPGDPVLFIEDGVYAVQAGNRFGALIQSVLKKNNPVYALKPDLDARGISAIAEGVKTV
DYAGFVDLVEEHQVNSWL
>Mature_98_residues
MLHTINKSPFENNTFTTCVRFLQPGDPVLFIEDGVYAVQAGNRFGALIQSVLKKNNPVYALKPDLDARGISAIAEGVKTV
DYAGFVDLVEEHQVNSWL

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions [H]

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family [H]

Homologues:

Organism=Escherichia coli, GI1789741, Length=93, Percent_Identity=38.7096774193548, Blast_Score=65, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007215 [H]

Pfam domain/function: PF04077 DsrH [H]

EC number: NA

Molecular weight: Translated: 10824; Mature: 10824

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTINKSPFENNTFTTCVRFLQPGDPVLFIEDGVYAVQAGNRFGALIQSVLKKNNPVYA
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCEEE
LKPDLDARGISAIAEGVKTVDYAGFVDLVEEHQVNSWL
ECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLHTINKSPFENNTFTTCVRFLQPGDPVLFIEDGVYAVQAGNRFGALIQSVLKKNNPVYA
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCEEE
LKPDLDARGISAIAEGVKTVDYAGFVDLVEEHQVNSWL
ECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]