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The map label for this gene is nudG [H]

Identifier: 21673459

GI number: 21673459

Start: 626294

End: 626800

Strand: Reverse

Name: nudG [H]

Synonym: CT0625

Alternate gene names: 21673459

Gene position: 626800-626294 (Counterclockwise)

Preceding gene: 21673462

Following gene: 21673458

Centisome position: 29.09

GC content: 58.19

Gene sequence:

>507_bases
TTGACACACCTCGCTGAACAGGCTCGCTTCATCATTGATTTTATCATAATTTTTTCAGCATTGACTACTTCATACCATAT
CGGCAACGTCGTGTGTGCCATCATCGAGCGCGAGGGCCGGTTCCTGATAGCCCGAAGGCCCTTGGGCAAGCATCTTGCGC
GGAAATGGGAGTTTCCGGGGAGCAAGGTGGAGACCGGCGAATCGGAAGCCGAAGCACTCGAACGGGAGCTGATAGAGGAG
CTTGGCGTCCGCATGGAGATTGTCGAACGCCTCATGCCGGTCGAGCACTGCTACGCCGACCGGTCGCTCCGGCTGATTGC
GTTTCACTGCCGTATCGCTGCTGGCGCTCCGAATGCCGGTGAGCACGAGGAGCTGCGATGGATCGACATCGGCGAGGCCG
ACGACTACGATTTTCCGGAGGCCGATTTGCCCATTCTTGCCGAGTACCGGCAGAAAATTGCCGCATCTGTACAGTCGTTG
CCCGGCAAGCGGCGCGGAACAGCGTGA

Upstream 100 bases:

>100_bases
TATTGCCAAAAAATGGACGATGCCGCTATGCAACCGGGGGGCGGTCATCAATCAGTTTTCCATTACATTCGAGGGAAGGG
TGACTCTATGAAAACGACCG

Downstream 100 bases:

>100_bases
TCGCAACGGAGCATTTCGCTTGCTCTTGTTGTCTTTATGGTGACTTGCAGATACCTCATCACCCATCAATCAAATACCCC
ATGCTGATCCTGAGTAAACT

Product: Nudix/MutT family protein

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTHLAEQARFIIDFIIIFSALTTSYHIGNVVCAIIEREGRFLIARRPLGKHLARKWEFPGSKVETGESEAEALERELIEE
LGVRMEIVERLMPVEHCYADRSLRLIAFHCRIAAGAPNAGEHEELRWIDIGEADDYDFPEADLPILAEYRQKIAASVQSL
PGKRRGTA

Sequences:

>Translated_168_residues
MTHLAEQARFIIDFIIIFSALTTSYHIGNVVCAIIEREGRFLIARRPLGKHLARKWEFPGSKVETGESEAEALERELIEE
LGVRMEIVERLMPVEHCYADRSLRLIAFHCRIAAGAPNAGEHEELRWIDIGEADDYDFPEADLPILAEYRQKIAASVQSL
PGKRRGTA
>Mature_167_residues
THLAEQARFIIDFIIIFSALTTSYHIGNVVCAIIEREGRFLIARRPLGKHLARKWEFPGSKVETGESEAEALERELIEEL
GVRMEIVERLMPVEHCYADRSLRLIAFHCRIAAGAPNAGEHEELRWIDIGEADDYDFPEADLPILAEYRQKIAASVQSLP
GKRRGTA

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=121, Percent_Identity=40.495867768595, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786288, Length=123, Percent_Identity=30.8943089430894, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19028; Mature: 18897

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLAEQARFIIDFIIIFSALTTSYHIGNVVCAIIEREGRFLIARRPLGKHLARKWEFPG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHCCCCC
SKVETGESEAEALERELIEELGVRMEIVERLMPVEHCYADRSLRLIAFHCRIAAGAPNAG
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
EHEELRWIDIGEADDYDFPEADLPILAEYRQKIAASVQSLPGKRRGTA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
THLAEQARFIIDFIIIFSALTTSYHIGNVVCAIIEREGRFLIARRPLGKHLARKWEFPG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHCCCCC
SKVETGESEAEALERELIEELGVRMEIVERLMPVEHCYADRSLRLIAFHCRIAAGAPNAG
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
EHEELRWIDIGEADDYDFPEADLPILAEYRQKIAASVQSLPGKRRGTA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]