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The map label for this gene is leuC [H]

Identifier: 21673449

GI number: 21673449

Start: 615073

End: 616368

Strand: Reverse

Name: leuC [H]

Synonym: CT0614

Alternate gene names: 21673449

Gene position: 616368-615073 (Counterclockwise)

Preceding gene: 21673450

Following gene: 21673448

Centisome position: 28.6

GC content: 61.65

Gene sequence:

>1296_bases
ATGGCACAAACGATAACCCAGAAAATTTTCGCCAGGGCTGCGAACCGCAAATTCGTCGATCCCGGCCAGAGCGTATGGCT
CAATGTCGATGTTCTCTTGACGCACGACGTCTGCGGCCCGCCGACCTTCGATATCTTCAAGCAGGAGTTCGGCCCGAACG
CCAAGGTGTGGGATCCGTCGAAAGTGGTGGTGCTTCCCGACCACTACATCTTCACGGCCAACGAGCACGCGCACCGCAAT
ATCGACCTCTTGCGCCAGTTCGCCGCCGAGCAGGGCTTGCCGAACTACTATGATGTAGGCACCGACCGCTACCGCGGCGT
CTGCCACGTCGCGCTGGCCGAAGAGGGCTTTAACCTCCCCGGCACCGTGCTGTTCGGCACCGACTCGCACACCTGCACCT
CGGGCGCATTCGGCATGTTCGGCTCCGGCATCGGCAACACCGACGCAGCCTTCATCCTCGGCACCGGCAAGCTCTGGGAG
AAGGTGCCTGATTCGATGAAGTTCACCTTTGAAGGCCAGATGCCCGAGTACCTGACGGCCAAAGACCTGATCCTCCAGAT
CCTCGGCGACATCACCACCGACGGCGCGACCTACCGCGCCATGGAGTTCGATGGCGAAGCGGTCTATTCGCTGCCGATCG
ACGAGCGCATGACGCTCTGCAACATGGCCATCGAGGCGGGCGGCATGAACGGTATCATCGCTGCCGATGCGGTCACCGAA
GCCTTCGTGAAGGCGCGCACCAGCAAGCCGTACGAAATCTTCACGAGCGATCCCGACGCGCAGTACCACAGCATGTACCG
CTACAACGTCGAAAAGATGGAGCCGATCGTCGCCAAGCCGCACAGCCCGGACAACCGCGCCACCGTGCACAGCGTGGCCG
GAACGCCGATCACCAAATCGTACATCGGCTCCTGTACCGGCGGCAAGCTGACCGACTTCAAGCTCGCAGCGAAGATTCTC
AAGGGCAAGAAGGTGGCGGTCACAACCAACATCGTCCCGGCAACCGTGCTCGTGGCATCGCAGCTCGAAACCGAGATGTA
CGATGGCCAGACACTGCGCCATATTTTCGAGGAAGCTGGCTGCAACATCGCCCTGCCATCGTGCGCGGCGTGTCTCGGCG
GCCCTTCGGACACGGTCGGGCGCTCGGTGGACAACGACGTCGTCGTTTCGACCACCAACCGCAACTTCCCTGGCCGCATG
GGCAGCAAGTTCGCGAGCGTCTATCTGGCCTCGCCGCTGACTGCGGCAGCCTCTGCCATAACGGGCAAACTCACCGATCC
GAGAGATTTCCTCTGA

Upstream 100 bases:

>100_bases
CTGCAGGTCTCCGGCTTTTCAGTTTTTGACGGGGCCTGCTAGCCCGGACAAGAAAAAATTCAGACAAGATTATATATCCG
ATAACGGAATCTTTGAAACC

Downstream 100 bases:

>100_bases
TCGAACGAAGCAAATGACAAGGAAAACACAGCATCATGGATACCATCATACAAGGTAAAGCCTACGTTCTCGGCAAGAAT
ATCGACACCGACCAGATCAT

Product: 3-isopropylmalate dehydratase, large subunit, putative

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQTITQKIFARAANRKFVDPGQSVWLNVDVLLTHDVCGPPTFDIFKQEFGPNAKVWDPSKVVVLPDHYIFTANEHAHRN
IDLLRQFAAEQGLPNYYDVGTDRYRGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWE
KVPDSMKFTFEGQMPEYLTAKDLILQILGDITTDGATYRAMEFDGEAVYSLPIDERMTLCNMAIEAGGMNGIIAADAVTE
AFVKARTSKPYEIFTSDPDAQYHSMYRYNVEKMEPIVAKPHSPDNRATVHSVAGTPITKSYIGSCTGGKLTDFKLAAKIL
KGKKVAVTTNIVPATVLVASQLETEMYDGQTLRHIFEEAGCNIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRM
GSKFASVYLASPLTAAASAITGKLTDPRDFL

Sequences:

>Translated_431_residues
MAQTITQKIFARAANRKFVDPGQSVWLNVDVLLTHDVCGPPTFDIFKQEFGPNAKVWDPSKVVVLPDHYIFTANEHAHRN
IDLLRQFAAEQGLPNYYDVGTDRYRGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWE
KVPDSMKFTFEGQMPEYLTAKDLILQILGDITTDGATYRAMEFDGEAVYSLPIDERMTLCNMAIEAGGMNGIIAADAVTE
AFVKARTSKPYEIFTSDPDAQYHSMYRYNVEKMEPIVAKPHSPDNRATVHSVAGTPITKSYIGSCTGGKLTDFKLAAKIL
KGKKVAVTTNIVPATVLVASQLETEMYDGQTLRHIFEEAGCNIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRM
GSKFASVYLASPLTAAASAITGKLTDPRDFL
>Mature_430_residues
AQTITQKIFARAANRKFVDPGQSVWLNVDVLLTHDVCGPPTFDIFKQEFGPNAKVWDPSKVVVLPDHYIFTANEHAHRNI
DLLRQFAAEQGLPNYYDVGTDRYRGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEK
VPDSMKFTFEGQMPEYLTAKDLILQILGDITTDGATYRAMEFDGEAVYSLPIDERMTLCNMAIEAGGMNGIIAADAVTEA
FVKARTSKPYEIFTSDPDAQYHSMYRYNVEKMEPIVAKPHSPDNRATVHSVAGTPITKSYIGSCTGGKLTDFKLAAKILK
GKKVAVTTNIVPATVLVASQLETEMYDGQTLRHIFEEAGCNIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMG
SKFASVYLASPLTAAASAITGKLTDPRDFL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=343, Percent_Identity=26.530612244898, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786259, Length=481, Percent_Identity=32.6403326403326, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI2367097, Length=476, Percent_Identity=26.0504201680672, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=24.2268041237113, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=341, Percent_Identity=27.5659824046921, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564738, Length=341, Percent_Identity=27.5659824046921, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25149342, Length=289, Percent_Identity=26.9896193771626, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568399, Length=380, Percent_Identity=25.5263157894737, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=485, Percent_Identity=30.5154639175258, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320440, Length=463, Percent_Identity=30.6695464362851, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323335, Length=343, Percent_Identity=27.1137026239067, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=372, Percent_Identity=25.5376344086022, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161076999, Length=366, Percent_Identity=25.4098360655738, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI281365315, Length=366, Percent_Identity=25.4098360655738, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17864292, Length=366, Percent_Identity=25.4098360655738, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28571643, Length=341, Percent_Identity=26.3929618768328, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137564, Length=367, Percent_Identity=25.3405994550409, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 46671; Mature: 46540

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTITQKIFARAANRKFVDPGQSVWLNVDVLLTHDVCGPPTFDIFKQEFGPNAKVWDPS
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCEECCC
KVVVLPDHYIFTANEHAHRNIDLLRQFAAEQGLPNYYDVGTDRYRGVCHVALAEEGFNLP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEKVPDSMKFTFEGQMPEYLTA
CEEEECCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHCCCCEEEEECCCCCCHHHH
KDLILQILGDITTDGATYRAMEFDGEAVYSLPIDERMTLCNMAIEAGGMNGIIAADAVTE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHH
AFVKARTSKPYEIFTSDPDAQYHSMYRYNVEKMEPIVAKPHSPDNRATVHSVAGTPITKS
HHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHCCCCEECCCCCCCCEEEEECCCCCCCHH
YIGSCTGGKLTDFKLAAKILKGKKVAVTTNIVPATVLVASQLETEMYDGQTLRHIFEEAG
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
CNIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMGSKFASVYLASPLTAAASAI
CCEECCHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC
>Mature Secondary Structure 
AQTITQKIFARAANRKFVDPGQSVWLNVDVLLTHDVCGPPTFDIFKQEFGPNAKVWDPS
CHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCEECCC
KVVVLPDHYIFTANEHAHRNIDLLRQFAAEQGLPNYYDVGTDRYRGVCHVALAEEGFNLP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGSGIGNTDAAFILGTGKLWEKVPDSMKFTFEGQMPEYLTA
CEEEECCCCCCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHCCCCEEEEECCCCCCHHHH
KDLILQILGDITTDGATYRAMEFDGEAVYSLPIDERMTLCNMAIEAGGMNGIIAADAVTE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHH
AFVKARTSKPYEIFTSDPDAQYHSMYRYNVEKMEPIVAKPHSPDNRATVHSVAGTPITKS
HHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHCCCCEECCCCCCCCEEEEECCCCCCCHH
YIGSCTGGKLTDFKLAAKILKGKKVAVTTNIVPATVLVASQLETEMYDGQTLRHIFEEAG
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
CNIALPSCAACLGGPSDTVGRSVDNDVVVSTTNRNFPGRMGSKFASVYLASPLTAAASAI
CCEECCHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]