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The map label for this gene is leuD1 [H]

Identifier: 21673448

GI number: 21673448

Start: 614465

End: 615037

Strand: Reverse

Name: leuD1 [H]

Synonym: CT0613

Alternate gene names: 21673448

Gene position: 615037-614465 (Counterclockwise)

Preceding gene: 21673449

Following gene: 21673447

Centisome position: 28.54

GC content: 56.54

Gene sequence:

>573_bases
ATGGATACCATCATACAAGGTAAAGCCTACGTTCTCGGCAAGAATATCGACACCGACCAGATCATTCCCGCCGAGCACCT
GGTCTACAGCCTCTCCGATCCGGAAGAGGTGAAGATGTACGGCAAATACGCGCTCTCCGGCGTTCCGATCGACCAGGCGG
GACTTCCGGAAGGCAATATTCCGTTCGTCGAAGAGGGTGAGTTCACCTCGCCATACTCGATCATCATCGCCGGACCCAAC
TTCGGTTGCGGCTCGTCGAGAGAGCACGCGCCCTTCGCGCTGAAGGTCGCCGGAGCCAAAGCGATCATCGCCGAATCCTA
CGCGAGGATTTTCTACCGCAACTGCGTGGACGGCGGTTTTGTTATTCCCTTTGAGACGGCGCAGCCGCTCAACAAGTCGA
TCATGACCGGCGACGAGCTGTCGCTCGACATGGAGAACAACACGCTGACCAACCTCACGCAGAACATCACCTACGAGCTG
CGGCCGCTGGGTGACGTGATCAATATTGTGCAGGCGGGCGGCATCTTTGAATACGCAAGGAAAAACAACCTCATGGCTTC
GACTGAGGCATAA

Upstream 100 bases:

>100_bases
CGCCGCTGACTGCGGCAGCCTCTGCCATAACGGGCAAACTCACCGATCCGAGAGATTTCCTCTGATCGAACGAAGCAAAT
GACAAGGAAAACACAGCATC

Downstream 100 bases:

>100_bases
GCAAAAGCATGGTAATAGAACTGTATGACACCACCCTGCGTGACGGTACGCAGGGTGAGCACATCAATCTTTCGGTACAG
GACAAGCTCTTGATCGCCGA

Product: 3-isopropylmalate dehydratase, small subunit, putative

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MDTIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKMYGKYALSGVPIDQAGLPEGNIPFVEEGEFTSPYSIIIAGPN
FGCGSSREHAPFALKVAGAKAIIAESYARIFYRNCVDGGFVIPFETAQPLNKSIMTGDELSLDMENNTLTNLTQNITYEL
RPLGDVINIVQAGGIFEYARKNNLMASTEA

Sequences:

>Translated_190_residues
MDTIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKMYGKYALSGVPIDQAGLPEGNIPFVEEGEFTSPYSIIIAGPN
FGCGSSREHAPFALKVAGAKAIIAESYARIFYRNCVDGGFVIPFETAQPLNKSIMTGDELSLDMENNTLTNLTQNITYEL
RPLGDVINIVQAGGIFEYARKNNLMASTEA
>Mature_190_residues
MDTIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKMYGKYALSGVPIDQAGLPEGNIPFVEEGEFTSPYSIIIAGPN
FGCGSSREHAPFALKVAGAKAIIAESYARIFYRNCVDGGFVIPFETAQPLNKSIMTGDELSLDMENNTLTNLTQNITYEL
RPLGDVINIVQAGGIFEYARKNNLMASTEA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=103, Percent_Identity=38.8349514563107, Blast_Score=64, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321429, Length=166, Percent_Identity=33.7349397590361, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20659; Mature: 20659

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKMYGKYALSGVPIDQAGLPEGNI
CCCEECCCEEEEECCCCCCCCCCHHHEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCCC
PFVEEGEFTSPYSIIIAGPNFGCGSSREHAPFALKVAGAKAIIAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
VIPFETAQPLNKSIMTGDELSLDMENNTLTNLTQNITYELRPLGDVINIVQAGGIFEYAR
EEEECCCCCCCHHHCCCCCEEEEECCCCHHHHHCCCEEEEECHHHHHHHHHCCCCHHHHH
KNNLMASTEA
CCCEEEECCC
>Mature Secondary Structure
MDTIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPEEVKMYGKYALSGVPIDQAGLPEGNI
CCCEECCCEEEEECCCCCCCCCCHHHEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCCC
PFVEEGEFTSPYSIIIAGPNFGCGSSREHAPFALKVAGAKAIIAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
VIPFETAQPLNKSIMTGDELSLDMENNTLTNLTQNITYELRPLGDVINIVQAGGIFEYAR
EEEECCCCCCCHHHCCCCCEEEEECCCCHHHHHCCCEEEEECHHHHHHHHHCCCCHHHHH
KNNLMASTEA
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]