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The map label for this gene is acn [H]

Identifier: 21673378

GI number: 21673378

Start: 550411

End: 552975

Strand: Reverse

Name: acn [H]

Synonym: CT0543

Alternate gene names: 21673378

Gene position: 552975-550411 (Counterclockwise)

Preceding gene: 21673380

Following gene: 21673377

Centisome position: 25.66

GC content: 61.09

Gene sequence:

>2565_bases
ATGAGTCTCATCAGCCAGTATCGCGCCCATACTGCGGAGCGCGCTCAACTCGGTATTCCTCCGCTTCCGCTGACCGCCGC
CCAGGCCATCGAGCTGATCGCGCTGCTCAAGCAGAATCCGGTGCAGGAGCAGGAGTATCTTCTCGATCTGTTCGTGAACC
ACATCAGCCCCGGCGTGGACGATGCCGCGCTCGAAAAGGCGGCTTTTCTCGATGCCATCATTCGCGGCAACGCTTCGTGC
GCGGTGATCACGCCAGTCGAGGCGGTCAGGATCCTCGGTACGATGCTTGGCGGTTACAACGTCAAGCCAATGATCGATGC
GCTCTCCAGTGCCGACAACGCGGTTGCCGAAGCCGCTGCTCTGGCCCTGAAAAAGACTCTGCTCGTCTATGATTCATTTG
ACACGGTGGTTGAGCTGGCCAAAACCAACAGCTACGCCAAAGAGGTGCTCGAATCGTGGGCTAATGGAGAATGGTTCACC
TCGAAGCCAGCCCTTGCCGAGAAGATGACCCTCACGGTCTTCAAGGTTCCGGGCGAAACCAACACCGACGACCTCTCTCC
CGCCAGCGAAGCCTTCACCCGCAGCGATATTCCGATGCACGCGCTGAGCATGCTCCGCTCGAAGATGGACGACCCGATCG
CGACCATCGCCCAGCTCAAGGAGAAGGGCTATCCGCTCGCGTACGTCGGCGACGTGGTCGGCACTGGCTCAAGCCGCAAG
TCGGGTATCAACTCCGTGCAGTGGCACCTCGGCGAGGATATTCCCGCCGTGCCGAACAAGCGCACCGCCGGCGTGGTGAT
CGGCGGCATCATCGCCCCGATCTTTTTCAACACCGCCGAAGACTCCGGCGCGCTGCCGATCCAGGCAGATGTGACCTCGA
TGGAAATGGGCGACGTGATCGACCTCTATCCGTTCAAAGGCGTGATCGAAAAGAACGGCAAAGTGATTTCGACCTTCTCG
CTCGAACCCAACACGCTTGCCGACGAAGTTCGCGCTGGCGGCCGTATTCCGCTTATCATCGGTCGCAACCTCACCCGCAA
GGCTCGCAAGACGCTCGGCCTCGGCGAAGAGTCGATCTTCAGCCGTCCCGAGCAGCCCGCCGATACCGGCAAGGGCTACA
CCCTGGCGCAGAAGATAGTCGGCAAGGCATGCGGCCTCCCTGGCGTTCGCCCCGGCATGTACGTCGAAGCCGAGACCCTC
ACGGTCGGCTCGCAAGACACCACCGGCCCGATGACCCGCGACGAAATCAAGGAGCTCGCTGCGCTGAGTTTCAGCGCCGA
TCTTTTCATGCAGAGCTTCTGCCACACGGCAGCTTACCCGAAACCCTCGGACGTGCAGCTGCACCGAAGCCTGCCCTATT
TTATCATGAGCCGCGGCGGCGTGGCACTCAAGCCGGGCGACGGCGTCATACACACCTGGCTGAACCGCATGGTGCTGCCC
GACACGCTCGGCACTGGCGGCGACTCGCACACCCGCTTCCCGATCGGCTGTTCCTTCCCGGCAGGCTCCGGCCTCGTCGC
CTTCGCAGGCGTGACTGGCACCATGCCGCTCAACATGCCGGAGTCGGTGCTCGTCCGCTTTACGGGTGAACTCCAGCCCG
GCATCACCCTGCGCGATCTGGTCAACGCCATTCCGTACGTCGCCATCAAGCGGGGGCTGCTGACCGTCGAGAAGAAGGGC
AAAAAGAACATCTTCGCCGGCAAAGTGCTTGAAATCGAAGGTTTGCCGCAGCTCAAGGTCGAGCAGGCGTTCGAGCTTTC
CGACGCCTCCGCCGAGCGCAGCGCTGCGGCCTGCACGGTGCGACTCGACAAGGAGCCGGTGATCGAGTACCTACAGTCCA
ACGTCAAATTGCTTGCCCAGATGATCGAAGAGGGCTACGGCGACGCCAACACCATCAAGCGCCGCATCGGCAAGATGGAG
GAGTGGCTCGCCAATCCGCAGCTCCTCGAACCGGACGCTGACGCCGAATACGCGGCAGTGATCGAGATCAACATGAGCGA
AATCACCGAACCGATCCTCGCCTGCCCGAATGATCCCGACGACGTGGCCACGCTCAGCGAGATTCTCGCCGACGAGAAGC
GCCCGAAAAACATCGACGAGGTGTTCGTGGGAAGCTGCATGACCAATATCGGCCACTTCCGCGCGCTTGGTGAAGTGCTT
CGTGGCAAGGGTCAGGCCAAAACCAGGCTCTGGGTGGTGCCGCCGACCAAGATGGACATGAAGAAGCTGATTGAAGAGGG
CTATTACTCAATTTTCGGCACCGCAGGCGCACGCACCGAGGTGCCCGGCTGTTCGCTCTGTATGGGCAACCAGGCTCGCG
TGGCCGACAACGCCGTGGTCTTCTCGACAAGCACTCGCAACTTCGACGACCGCATGGGCAAGGGCGCGAAGGTCTATCTC
GGCTCCGCCGAACTGGCCGCCGTCTGTGCTTTGCTCGGCCATCTGCCCAGCAAGGATGAGTATATGGAGATCGCCGGTTC
GCTGTCGAAGAACAGTGACAAGGTTTACCGTTACCTCAACTTCCATGAGGTCACCTCTCAGGAGCTACAACTGCTTGTCG
ATTAA

Upstream 100 bases:

>100_bases
GCTTTTTGCTTTTTTCAAGGCAAAAACCATTCCGGTTGTTACCGTTCGCTGTAATCACCATCGAATAATCATTCATAATC
ATTTCTCTGGAGATCATTAT

Downstream 100 bases:

>100_bases
TCGATTACAGGTTTCCACCTCCCTCCGTCTTTGTGCAGGAGAGGAGGTGGAGACGCTTCTGTCGTATGCCTTTTGCTGCT
GATCTTTGTTTTTGAATGCC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 854; Mature: 853

Protein sequence:

>854_residues
MSLISQYRAHTAERAQLGIPPLPLTAAQAIELIALLKQNPVQEQEYLLDLFVNHISPGVDDAALEKAAFLDAIIRGNASC
AVITPVEAVRILGTMLGGYNVKPMIDALSSADNAVAEAAALALKKTLLVYDSFDTVVELAKTNSYAKEVLESWANGEWFT
SKPALAEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPMHALSMLRSKMDDPIATIAQLKEKGYPLAYVGDVVGTGSSRK
SGINSVQWHLGEDIPAVPNKRTAGVVIGGIIAPIFFNTAEDSGALPIQADVTSMEMGDVIDLYPFKGVIEKNGKVISTFS
LEPNTLADEVRAGGRIPLIIGRNLTRKARKTLGLGEESIFSRPEQPADTGKGYTLAQKIVGKACGLPGVRPGMYVEAETL
TVGSQDTTGPMTRDEIKELAALSFSADLFMQSFCHTAAYPKPSDVQLHRSLPYFIMSRGGVALKPGDGVIHTWLNRMVLP
DTLGTGGDSHTRFPIGCSFPAGSGLVAFAGVTGTMPLNMPESVLVRFTGELQPGITLRDLVNAIPYVAIKRGLLTVEKKG
KKNIFAGKVLEIEGLPQLKVEQAFELSDASAERSAAACTVRLDKEPVIEYLQSNVKLLAQMIEEGYGDANTIKRRIGKME
EWLANPQLLEPDADAEYAAVIEINMSEITEPILACPNDPDDVATLSEILADEKRPKNIDEVFVGSCMTNIGHFRALGEVL
RGKGQAKTRLWVVPPTKMDMKKLIEEGYYSIFGTAGARTEVPGCSLCMGNQARVADNAVVFSTSTRNFDDRMGKGAKVYL
GSAELAAVCALLGHLPSKDEYMEIAGSLSKNSDKVYRYLNFHEVTSQELQLLVD

Sequences:

>Translated_854_residues
MSLISQYRAHTAERAQLGIPPLPLTAAQAIELIALLKQNPVQEQEYLLDLFVNHISPGVDDAALEKAAFLDAIIRGNASC
AVITPVEAVRILGTMLGGYNVKPMIDALSSADNAVAEAAALALKKTLLVYDSFDTVVELAKTNSYAKEVLESWANGEWFT
SKPALAEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPMHALSMLRSKMDDPIATIAQLKEKGYPLAYVGDVVGTGSSRK
SGINSVQWHLGEDIPAVPNKRTAGVVIGGIIAPIFFNTAEDSGALPIQADVTSMEMGDVIDLYPFKGVIEKNGKVISTFS
LEPNTLADEVRAGGRIPLIIGRNLTRKARKTLGLGEESIFSRPEQPADTGKGYTLAQKIVGKACGLPGVRPGMYVEAETL
TVGSQDTTGPMTRDEIKELAALSFSADLFMQSFCHTAAYPKPSDVQLHRSLPYFIMSRGGVALKPGDGVIHTWLNRMVLP
DTLGTGGDSHTRFPIGCSFPAGSGLVAFAGVTGTMPLNMPESVLVRFTGELQPGITLRDLVNAIPYVAIKRGLLTVEKKG
KKNIFAGKVLEIEGLPQLKVEQAFELSDASAERSAAACTVRLDKEPVIEYLQSNVKLLAQMIEEGYGDANTIKRRIGKME
EWLANPQLLEPDADAEYAAVIEINMSEITEPILACPNDPDDVATLSEILADEKRPKNIDEVFVGSCMTNIGHFRALGEVL
RGKGQAKTRLWVVPPTKMDMKKLIEEGYYSIFGTAGARTEVPGCSLCMGNQARVADNAVVFSTSTRNFDDRMGKGAKVYL
GSAELAAVCALLGHLPSKDEYMEIAGSLSKNSDKVYRYLNFHEVTSQELQLLVD
>Mature_853_residues
SLISQYRAHTAERAQLGIPPLPLTAAQAIELIALLKQNPVQEQEYLLDLFVNHISPGVDDAALEKAAFLDAIIRGNASCA
VITPVEAVRILGTMLGGYNVKPMIDALSSADNAVAEAAALALKKTLLVYDSFDTVVELAKTNSYAKEVLESWANGEWFTS
KPALAEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPMHALSMLRSKMDDPIATIAQLKEKGYPLAYVGDVVGTGSSRKS
GINSVQWHLGEDIPAVPNKRTAGVVIGGIIAPIFFNTAEDSGALPIQADVTSMEMGDVIDLYPFKGVIEKNGKVISTFSL
EPNTLADEVRAGGRIPLIIGRNLTRKARKTLGLGEESIFSRPEQPADTGKGYTLAQKIVGKACGLPGVRPGMYVEAETLT
VGSQDTTGPMTRDEIKELAALSFSADLFMQSFCHTAAYPKPSDVQLHRSLPYFIMSRGGVALKPGDGVIHTWLNRMVLPD
TLGTGGDSHTRFPIGCSFPAGSGLVAFAGVTGTMPLNMPESVLVRFTGELQPGITLRDLVNAIPYVAIKRGLLTVEKKGK
KNIFAGKVLEIEGLPQLKVEQAFELSDASAERSAAACTVRLDKEPVIEYLQSNVKLLAQMIEEGYGDANTIKRRIGKMEE
WLANPQLLEPDADAEYAAVIEINMSEITEPILACPNDPDDVATLSEILADEKRPKNIDEVFVGSCMTNIGHFRALGEVLR
GKGQAKTRLWVVPPTKMDMKKLIEEGYYSIFGTAGARTEVPGCSLCMGNQARVADNAVVFSTSTRNFDDRMGKGAKVYLG
SAELAAVCALLGHLPSKDEYMEIAGSLSKNSDKVYRYLNFHEVTSQELQLLVD

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=858, Percent_Identity=63.2867132867133, Blast_Score=1082, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=386, Percent_Identity=26.9430051813472, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92164; Mature: 92033

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLISQYRAHTAERAQLGIPPLPLTAAQAIELIALLKQNPVQEQEYLLDLFVNHISPGVD
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DAALEKAAFLDAIIRGNASCAVITPVEAVRILGTMLGGYNVKPMIDALSSADNAVAEAAA
HHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
LALKKTLLVYDSFDTVVELAKTNSYAKEVLESWANGEWFTSKPALAEKMTLTVFKVPGET
HHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHCEEEEEEECCCCC
NTDDLSPASEAFTRSDIPMHALSMLRSKMDDPIATIAQLKEKGYPLAYVGDVVGTGSSRK
CCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECHHHCCCCCCC
SGINSVQWHLGEDIPAVPNKRTAGVVIGGIIAPIFFNTAEDSGALPIQADVTSMEMGDVI
CCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEECCCHHCCCCEE
DLYPFKGVIEKNGKVISTFSLEPNTLADEVRAGGRIPLIIGRNLTRKARKTLGLGEESIF
EECCCCCCEECCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHH
SRPEQPADTGKGYTLAQKIVGKACGLPGVRPGMYVEAETLTVGSQDTTGPMTRDEIKELA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
ALSFSADLFMQSFCHTAAYPKPSDVQLHRSLPYFIMSRGGVALKPGDGVIHTWLNRMVLP
HHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEECCCCEEECCCCCHHHHHHCCEECC
DTLGTGGDSHTRFPIGCSFPAGSGLVAFAGVTGTMPLNMPESVLVRFTGELQPGITLRDL
CCCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCHHHHEEEECCCCCCCCHHHH
VNAIPYVAIKRGLLTVEKKGKKNIFAGKVLEIEGLPQLKVEQAFELSDASAERSAAACTV
HHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEE
RLDKEPVIEYLQSNVKLLAQMIEEGYGDANTIKRRIGKMEEWLANPQLLEPDADAEYAAV
EECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEE
IEINMSEITEPILACPNDPDDVATLSEILADEKRPKNIDEVFVGSCMTNIGHFRALGEVL
EEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGKGQAKTRLWVVPPTKMDMKKLIEEGYYSIFGTAGARTEVPGCSLCMGNQARVADNAVV
HCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCEECCCEEE
FSTSTRNFDDRMGKGAKVYLGSAELAAVCALLGHLPSKDEYMEIAGSLSKNSDKVYRYLN
EECCCCCHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHH
FHEVTSQELQLLVD
HHHHCHHHHHEECC
>Mature Secondary Structure 
SLISQYRAHTAERAQLGIPPLPLTAAQAIELIALLKQNPVQEQEYLLDLFVNHISPGVD
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
DAALEKAAFLDAIIRGNASCAVITPVEAVRILGTMLGGYNVKPMIDALSSADNAVAEAAA
HHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
LALKKTLLVYDSFDTVVELAKTNSYAKEVLESWANGEWFTSKPALAEKMTLTVFKVPGET
HHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHCEEEEEEECCCCC
NTDDLSPASEAFTRSDIPMHALSMLRSKMDDPIATIAQLKEKGYPLAYVGDVVGTGSSRK
CCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECHHHCCCCCCC
SGINSVQWHLGEDIPAVPNKRTAGVVIGGIIAPIFFNTAEDSGALPIQADVTSMEMGDVI
CCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCEEEECCCHHCCCCEE
DLYPFKGVIEKNGKVISTFSLEPNTLADEVRAGGRIPLIIGRNLTRKARKTLGLGEESIF
EECCCCCCEECCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHH
SRPEQPADTGKGYTLAQKIVGKACGLPGVRPGMYVEAETLTVGSQDTTGPMTRDEIKELA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
ALSFSADLFMQSFCHTAAYPKPSDVQLHRSLPYFIMSRGGVALKPGDGVIHTWLNRMVLP
HHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEECCCCEEECCCCCHHHHHHCCEECC
DTLGTGGDSHTRFPIGCSFPAGSGLVAFAGVTGTMPLNMPESVLVRFTGELQPGITLRDL
CCCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCHHHHEEEECCCCCCCCHHHH
VNAIPYVAIKRGLLTVEKKGKKNIFAGKVLEIEGLPQLKVEQAFELSDASAERSAAACTV
HHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEE
RLDKEPVIEYLQSNVKLLAQMIEEGYGDANTIKRRIGKMEEWLANPQLLEPDADAEYAAV
EECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEE
IEINMSEITEPILACPNDPDDVATLSEILADEKRPKNIDEVFVGSCMTNIGHFRALGEVL
EEEEHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGKGQAKTRLWVVPPTKMDMKKLIEEGYYSIFGTAGARTEVPGCSLCMGNQARVADNAVV
HCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCEECCCEEE
FSTSTRNFDDRMGKGAKVYLGSAELAAVCALLGHLPSKDEYMEIAGSLSKNSDKVYRYLN
EECCCCCHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHH
FHEVTSQELQLLVD
HHHHCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]