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The map label for this gene is gltD-1 [H]

Identifier: 21673308

GI number: 21673308

Start: 489251

End: 490669

Strand: Reverse

Name: gltD-1 [H]

Synonym: CT0473

Alternate gene names: 21673308

Gene position: 490669-489251 (Counterclockwise)

Preceding gene: 21673309

Following gene: 21673307

Centisome position: 22.77

GC content: 61.66

Gene sequence:

>1419_bases
ATGGCCATTCCCCGTCAGAAGATGCCCGCTCAGGATCCAGTGGAGCGCGTCGGTAACTTCAAGGAGGTCAACCTTGGCCT
AACCCCGGAACAGGCACAGCAGGAAGCCCTGCGCTGTATCCAGTGCAAGGACCCGGTCTGCATCGCAGGGTGTCCGGTCA
ACATCAAGATCGACCAGTTCATCAAACTGATCGCCGAGGGTGACTTCATGGGCGCGGTCAGAAAGATCAAGGAGGACAAC
GTACTGCCGTCAATTTGCGGCCGCGTCTGCCCGCAGGAAGACCAGTGCGAGAAGGTGTGCGTCATCGGCAAGAAGCACGA
GCCGGTGGCCATCGGTAACCTCGAACGCTTCGTGGGCGATTACGAACGCACCAGCGGCCAGAAGATCGATCCGAAAATCG
CGCCTCCGACCGGCAAAAAGGTTGCCGTTGTCGGCAGTGGTCCGGCCGGCCTGAGCTGCGCCAATGACCTGGCCCAGTAC
GGCCACAAGGTGGTGGTGTTCGAGGCGCTGCACGAACTCGGCGGTGTTTTGATGTACGGCATTCCGGAATTCCGTCTGCC
GAAGGAGATCGTCCGCGAAGAGCTTGACGGCCTGCGCCGCATGGGCATCGAGTTCCGCACCGACGTGGTGGTCGGACGCA
CGATCACCATCGACGAGCTGATGGAAGAGGAAGGTTTCGACGCGGTTTTCATCGGCGTGGGCGCGGGTCTGCCCTGGTTC
ATGGGCATTCCGGGCGAGAACCTCGTCGGCGTACTCGCGGCCAACGAGTTCCTCACCAGGGTGAACCTCATGAAGGCCTA
CGACTTCCTGAAGAGCAGCGACACGCCGGTGTTCGACTGCAAGGGTAAAAACGTCGCGGTCTTCGGCGGCGGCAACACCG
CGATGGACGCCGTACGCACGGCCAAACGGCTCGGAGCGGAGCACGCCTACATCGTCTATCGCCGCTCCGAAAAAGAGATG
CCCGCCCGCGAGGAGGAGATTCACAACGCCAAGGAGGAGGGCATCGAGTTCCTGCTGCTCACCACACCGCTTGAATTCGT
CGGCGATGAAAAGGCGTGGCTCACCGGCGCGAAGTGCCAGAAAATGGAGCTGGGTGAACCCGACGATTCGGGACGCCGCC
GCCCGGTACCAGTCGAAGGCTCGGAGTACATCCTGCCCATCGACATGGCGATCATCTCCATCGGCAACGGCCCCAACCCG
CTCATCCACCAGACGACACCCGACATCGAGGTCAGCAAACGCGAGACCATCGTCGTTGACGTCAACACCATGCAGACCTC
CAAGGAGAACGTTTACGCCGGTGGCGACATCGTCACGGGCGGCGCGACCGTGATTCTCGCGATGGGCGCGGGACGCAAAG
CGGCGGCTGCCATCAACGAAAAGCTCGGCGGCACGGCCAAGAACTTCAACGAGTGGTAA

Upstream 100 bases:

>100_bases
CGAGCGACCTCCGGCGGGCAGGACTGTCACGAAAATCCGTTTCACCGCTAAACCAGCAATCGTAATCATGGACGCTCGTA
CTATCACCACCAAGGAACGT

Downstream 100 bases:

>100_bases
GCACGTGAGCGACCTCTACCGTTTCGGCATTTCGCTTGAAAGGAAACTGATCGAGTCTTTCGACCGGCACATCAAGGCCC
AGGGCTACCAGAGCCGGTCG

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAIPRQKMPAQDPVERVGNFKEVNLGLTPEQAQQEALRCIQCKDPVCIAGCPVNIKIDQFIKLIAEGDFMGAVRKIKEDN
VLPSICGRVCPQEDQCEKVCVIGKKHEPVAIGNLERFVGDYERTSGQKIDPKIAPPTGKKVAVVGSGPAGLSCANDLAQY
GHKVVVFEALHELGGVLMYGIPEFRLPKEIVREELDGLRRMGIEFRTDVVVGRTITIDELMEEEGFDAVFIGVGAGLPWF
MGIPGENLVGVLAANEFLTRVNLMKAYDFLKSSDTPVFDCKGKNVAVFGGGNTAMDAVRTAKRLGAEHAYIVYRRSEKEM
PAREEEIHNAKEEGIEFLLLTTPLEFVGDEKAWLTGAKCQKMELGEPDDSGRRRPVPVEGSEYILPIDMAIISIGNGPNP
LIHQTTPDIEVSKRETIVVDVNTMQTSKENVYAGGDIVTGGATVILAMGAGRKAAAAINEKLGGTAKNFNEW

Sequences:

>Translated_472_residues
MAIPRQKMPAQDPVERVGNFKEVNLGLTPEQAQQEALRCIQCKDPVCIAGCPVNIKIDQFIKLIAEGDFMGAVRKIKEDN
VLPSICGRVCPQEDQCEKVCVIGKKHEPVAIGNLERFVGDYERTSGQKIDPKIAPPTGKKVAVVGSGPAGLSCANDLAQY
GHKVVVFEALHELGGVLMYGIPEFRLPKEIVREELDGLRRMGIEFRTDVVVGRTITIDELMEEEGFDAVFIGVGAGLPWF
MGIPGENLVGVLAANEFLTRVNLMKAYDFLKSSDTPVFDCKGKNVAVFGGGNTAMDAVRTAKRLGAEHAYIVYRRSEKEM
PAREEEIHNAKEEGIEFLLLTTPLEFVGDEKAWLTGAKCQKMELGEPDDSGRRRPVPVEGSEYILPIDMAIISIGNGPNP
LIHQTTPDIEVSKRETIVVDVNTMQTSKENVYAGGDIVTGGATVILAMGAGRKAAAAINEKLGGTAKNFNEW
>Mature_471_residues
AIPRQKMPAQDPVERVGNFKEVNLGLTPEQAQQEALRCIQCKDPVCIAGCPVNIKIDQFIKLIAEGDFMGAVRKIKEDNV
LPSICGRVCPQEDQCEKVCVIGKKHEPVAIGNLERFVGDYERTSGQKIDPKIAPPTGKKVAVVGSGPAGLSCANDLAQYG
HKVVVFEALHELGGVLMYGIPEFRLPKEIVREELDGLRRMGIEFRTDVVVGRTITIDELMEEEGFDAVFIGVGAGLPWFM
GIPGENLVGVLAANEFLTRVNLMKAYDFLKSSDTPVFDCKGKNVAVFGGGNTAMDAVRTAKRLGAEHAYIVYRRSEKEMP
AREEEIHNAKEEGIEFLLLTTPLEFVGDEKAWLTGAKCQKMELGEPDDSGRRRPVPVEGSEYILPIDMAIISIGNGPNPL
IHQTTPDIEVSKRETIVVDVNTMQTSKENVYAGGDIVTGGATVILAMGAGRKAAAAINEKLGGTAKNFNEW

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=464, Percent_Identity=30.3879310344828, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI237757300, Length=103, Percent_Identity=37.8640776699029, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=38.7580299785867, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1789606, Length=454, Percent_Identity=39.647577092511, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87082180, Length=445, Percent_Identity=39.7752808988764, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1788468, Length=460, Percent_Identity=30, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1789243, Length=438, Percent_Identity=31.0502283105023, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=485, Percent_Identity=28.2474226804124, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71984108, Length=454, Percent_Identity=30.1762114537445, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17543860, Length=183, Percent_Identity=30.6010928961749, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=489, Percent_Identity=31.2883435582822, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=31.340206185567, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=31.340206185567, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=31.340206185567, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=31.340206185567, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24640763, Length=471, Percent_Identity=31.2101910828025, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI18858217, Length=471, Percent_Identity=31.2101910828025, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 51395; Mature: 51264

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRQKMPAQDPVERVGNFKEVNLGLTPEQAQQEALRCIQCKDPVCIAGCPVNIKIDQF
CCCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHH
IKLIAEGDFMGAVRKIKEDNVLPSICGRVCPQEDQCEKVCVIGKKHEPVAIGNLERFVGD
HHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCHHCCCCEEEECCCCCCEEECCHHHHHHH
YERTSGQKIDPKIAPPTGKKVAVVGSGPAGLSCANDLAQYGHKVVVFEALHELGGVLMYG
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEHHHHHHHCCEEEEC
IPEFRLPKEIVREELDGLRRMGIEFRTDVVVGRTITIDELMEEEGFDAVFIGVGAGLPWF
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEHHHHHHHCCCCEEEEECCCCCCEE
MGIPGENLVGVLAANEFLTRVNLMKAYDFLKSSDTPVFDCKGKNVAVFGGGNTAMDAVRT
ECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHHHHHH
AKRLGAEHAYIVYRRSEKEMPAREEEIHNAKEEGIEFLLLTTPLEFVGDEKAWLTGAKCQ
HHHHCCCCEEEEEECCCCCCCCHHHHHHCHHHCCCEEEEEECCHHHHCCCCCEEECCCCC
KMELGEPDDSGRRRPVPVEGSEYILPIDMAIISIGNGPNPLIHQTTPDIEVSKRETIVVD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCCCEECCCCEEEEE
VNTMQTSKENVYAGGDIVTGGATVILAMGAGRKAAAAINEKLGGTAKNFNEW
ECCCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AIPRQKMPAQDPVERVGNFKEVNLGLTPEQAQQEALRCIQCKDPVCIAGCPVNIKIDQF
CCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHH
IKLIAEGDFMGAVRKIKEDNVLPSICGRVCPQEDQCEKVCVIGKKHEPVAIGNLERFVGD
HHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCHHCCCCEEEECCCCCCEEECCHHHHHHH
YERTSGQKIDPKIAPPTGKKVAVVGSGPAGLSCANDLAQYGHKVVVFEALHELGGVLMYG
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEHHHHHHHCCEEEEC
IPEFRLPKEIVREELDGLRRMGIEFRTDVVVGRTITIDELMEEEGFDAVFIGVGAGLPWF
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEHHHHHHHCCCCEEEEECCCCCCEE
MGIPGENLVGVLAANEFLTRVNLMKAYDFLKSSDTPVFDCKGKNVAVFGGGNTAMDAVRT
ECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHHHHHH
AKRLGAEHAYIVYRRSEKEMPAREEEIHNAKEEGIEFLLLTTPLEFVGDEKAWLTGAKCQ
HHHHCCCCEEEEEECCCCCCCCHHHHHHCHHHCCCEEEEEECCHHHHCCCCCEEECCCCC
KMELGEPDDSGRRRPVPVEGSEYILPIDMAIISIGNGPNPLIHQTTPDIEVSKRETIVVD
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCCCEECCCCEEEEE
VNTMQTSKENVYAGGDIVTGGATVILAMGAGRKAAAAINEKLGGTAKNFNEW
ECCCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]