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The map label for this gene is gspE [C]

Identifier: 21673273

GI number: 21673273

Start: 453819

End: 455261

Strand: Direct

Name: gspE [C]

Synonym: CT0434

Alternate gene names: 21673273

Gene position: 453819-455261 (Clockwise)

Preceding gene: 21673272

Following gene: 21673274

Centisome position: 21.06

GC content: 51.49

Gene sequence:

>1443_bases
ATGGGAACGCTTAAAGATCAAATAGCTCGCTGGAATCTCTCCCTCAAAAGAGGAGCAGATTTTTCTTCCTCAGCCCCCGA
GACCGCTGATGTGCCTGAGAAAGCGCCTGTCGTTCAGGCTCCGGTAGCGTCAGTTCCCGTAGCAGCGAAAAAAAACAAGG
AATCCAACGGCGATCCTGACTATTACTCGGCCAAGACAAAGCTGCACCAGAAGCTCCTCACCCGCATCGATCTCAACTCG
ATAGAGAGCCTCGATGCCGATCAGTTGCGCAATGAGCTGGGTATGCTGCTTACCAGATTGATCGAAGAGGAGGCGCTGCC
CCTCAATCATCAGGAGCGCAGCAAATTGGTGACCGACCTCAAAAATGAAATCCTGGGTTTGGGTCCTTTGGAGCCTTTGC
TGGCAGACCCTGACATTTCTGAAATCATGGTCAACGGTTACCAGAATGTCTATGTAGAAAAAAAAGGATGCATTTCCTTG
ACCGACATTCGCTTCAGCGACGATGCTCACCTCATGAAAATCATAGACAAGATCGTCTCTCACGTGGGGCGGCGTATCGA
TGAATCAAGCCCCATGGTCGATGCCCGCCTTCCCGATGGTTCGCGCGTCAACGCCATCATTCCGCCATTGGCACTCGACG
GCCCTGCACTTACCATAAGGCGCTTTGCGGTTGTGCCTCTGCAAATGCACGACCTCATCGAAAAAAAAACACTTACGCCA
ACCATGGCCGAACTGTTGTCGGCTCTGGTGAAAGTCAAGTGCAACATCATTATCTCAGGTGGTACCGGCTCGGGAAAAAC
CACGTTGCTCAACATTCTTTCGGGTTATATTCCGTACAACGAGCGTATCATCACCATCGAAGACACTGCCGAGCTTCAGC
TTCAGCAGGATCATGTTATTCGCCTGGAGACGCGCCCGCCAAACATTGAAAATAAAGGTGAAGTCACCATGCGCGCGCTG
GTGAAAAACTCTCTGCGCATGAGGCCAGACCGCATCGTGCTGGGTGAGGTTCGTAGCTCCGAGGTGATCGACATGCTGCA
GGCCATGAACACCGGCCACGATGGTTCACTCACCACCATTCACGCCAATAGTCCACGTGATGCGATAGCCCGACTTGAAA
ACCTTGTGGGACTTGGTGGTATCAATCTGCCCAGCAAGGCGTTGCGTCAGCTTATCGCCTCTTCGGTTCAATTTATCATC
CAGGTTTCGCGACTCAGTGATGGTACCCGAAAGATCACCCATATCCAGGAGATCATCGGCATGGAAGGCGATGTCATTAC
CACACAGGATATCTTTTTTTTCCAGCGTACCGGCACTAATTCGGACGGATCTGTTCAGGGAGTGTTCAAAGCTACGGGCG
TGCGTCCCAGAGTTTATGAAAAAATACGCACTTTCGGTCTCAATCTTAGCGAAGGGATCTTTGACCCCGACTCCAAAGAC
TAA

Upstream 100 bases:

>100_bases
AGTCAGTGCTGAAAGTTGCGCCCAAATCCCAGCTTTCCAAAACCATCGTTGACTGGGCCTTGCACCTCAACGGCGTCAGT
CGGCCTAATAAACGCTCGAT

Downstream 100 bases:

>100_bases
ACTTTGTGCACATCATGACCACGTTTTTGTTATCATTATCTGTTTTTTTTGCGGTTCTGCTGATCATGTGGTTGGTGTAT
TTGGTTTGGTCCCGGTTTTT

Product: type II secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MGTLKDQIARWNLSLKRGADFSSSAPETADVPEKAPVVQAPVASVPVAAKKNKESNGDPDYYSAKTKLHQKLLTRIDLNS
IESLDADQLRNELGMLLTRLIEEEALPLNHQERSKLVTDLKNEILGLGPLEPLLADPDISEIMVNGYQNVYVEKKGCISL
TDIRFSDDAHLMKIIDKIVSHVGRRIDESSPMVDARLPDGSRVNAIIPPLALDGPALTIRRFAVVPLQMHDLIEKKTLTP
TMAELLSALVKVKCNIIISGGTGSGKTTLLNILSGYIPYNERIITIEDTAELQLQQDHVIRLETRPPNIENKGEVTMRAL
VKNSLRMRPDRIVLGEVRSSEVIDMLQAMNTGHDGSLTTIHANSPRDAIARLENLVGLGGINLPSKALRQLIASSVQFII
QVSRLSDGTRKITHIQEIIGMEGDVITTQDIFFFQRTGTNSDGSVQGVFKATGVRPRVYEKIRTFGLNLSEGIFDPDSKD

Sequences:

>Translated_480_residues
MGTLKDQIARWNLSLKRGADFSSSAPETADVPEKAPVVQAPVASVPVAAKKNKESNGDPDYYSAKTKLHQKLLTRIDLNS
IESLDADQLRNELGMLLTRLIEEEALPLNHQERSKLVTDLKNEILGLGPLEPLLADPDISEIMVNGYQNVYVEKKGCISL
TDIRFSDDAHLMKIIDKIVSHVGRRIDESSPMVDARLPDGSRVNAIIPPLALDGPALTIRRFAVVPLQMHDLIEKKTLTP
TMAELLSALVKVKCNIIISGGTGSGKTTLLNILSGYIPYNERIITIEDTAELQLQQDHVIRLETRPPNIENKGEVTMRAL
VKNSLRMRPDRIVLGEVRSSEVIDMLQAMNTGHDGSLTTIHANSPRDAIARLENLVGLGGINLPSKALRQLIASSVQFII
QVSRLSDGTRKITHIQEIIGMEGDVITTQDIFFFQRTGTNSDGSVQGVFKATGVRPRVYEKIRTFGLNLSEGIFDPDSKD
>Mature_479_residues
GTLKDQIARWNLSLKRGADFSSSAPETADVPEKAPVVQAPVASVPVAAKKNKESNGDPDYYSAKTKLHQKLLTRIDLNSI
ESLDADQLRNELGMLLTRLIEEEALPLNHQERSKLVTDLKNEILGLGPLEPLLADPDISEIMVNGYQNVYVEKKGCISLT
DIRFSDDAHLMKIIDKIVSHVGRRIDESSPMVDARLPDGSRVNAIIPPLALDGPALTIRRFAVVPLQMHDLIEKKTLTPT
MAELLSALVKVKCNIIISGGTGSGKTTLLNILSGYIPYNERIITIEDTAELQLQQDHVIRLETRPPNIENKGEVTMRALV
KNSLRMRPDRIVLGEVRSSEVIDMLQAMNTGHDGSLTTIHANSPRDAIARLENLVGLGGINLPSKALRQLIASSVQFIIQ
VSRLSDGTRKITHIQEIIGMEGDVITTQDIFFFQRTGTNSDGSVQGVFKATGVRPRVYEKIRTFGLNLSEGIFDPDSKD

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52854; Mature: 52722

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLKDQIARWNLSLKRGADFSSSAPETADVPEKAPVVQAPVASVPVAAKKNKESNGDPD
CCCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCC
YYSAKTKLHQKLLTRIDLNSIESLDADQLRNELGMLLTRLIEEEALPLNHQERSKLVTDL
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KNEILGLGPLEPLLADPDISEIMVNGYQNVYVEKKGCISLTDIRFSDDAHLMKIIDKIVS
HHHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHH
HVGRRIDESSPMVDARLPDGSRVNAIIPPLALDGPALTIRRFAVVPLQMHDLIEKKTLTP
HHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCHHEEEHHEECCHHHHHHHHHHCCCH
TMAELLSALVKVKCNIIISGGTGSGKTTLLNILSGYIPYNERIITIEDTAELQLQQDHVI
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCEE
RLETRPPNIENKGEVTMRALVKNSLRMRPDRIVLGEVRSSEVIDMLQAMNTGHDGSLTTI
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEE
HANSPRDAIARLENLVGLGGINLPSKALRQLIASSVQFIIQVSRLSDGTRKITHIQEIIG
ECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
MEGDVITTQDIFFFQRTGTNSDGSVQGVFKATGVRPRVYEKIRTFGLNLSEGIFDPDSKD
CCCCEEEECEEEEEEECCCCCCCCEEEEHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCCC
>Mature Secondary Structure 
GTLKDQIARWNLSLKRGADFSSSAPETADVPEKAPVVQAPVASVPVAAKKNKESNGDPD
CCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCC
YYSAKTKLHQKLLTRIDLNSIESLDADQLRNELGMLLTRLIEEEALPLNHQERSKLVTDL
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KNEILGLGPLEPLLADPDISEIMVNGYQNVYVEKKGCISLTDIRFSDDAHLMKIIDKIVS
HHHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHH
HVGRRIDESSPMVDARLPDGSRVNAIIPPLALDGPALTIRRFAVVPLQMHDLIEKKTLTP
HHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCHHEEEHHEECCHHHHHHHHHHCCCH
TMAELLSALVKVKCNIIISGGTGSGKTTLLNILSGYIPYNERIITIEDTAELQLQQDHVI
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCEE
RLETRPPNIENKGEVTMRALVKNSLRMRPDRIVLGEVRSSEVIDMLQAMNTGHDGSLTTI
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEE
HANSPRDAIARLENLVGLGGINLPSKALRQLIASSVQFIIQVSRLSDGTRKITHIQEIIG
ECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
MEGDVITTQDIFFFQRTGTNSDGSVQGVFKATGVRPRVYEKIRTFGLNLSEGIFDPDSKD
CCCCEEEECEEEEEEECCCCCCCCEEEEHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]