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The map label for this gene is btuB [H]

Identifier: 21673262

GI number: 21673262

Start: 443994

End: 446333

Strand: Direct

Name: btuB [H]

Synonym: CT0423

Alternate gene names: 21673262

Gene position: 443994-446333 (Clockwise)

Preceding gene: 21673261

Following gene: 21673264

Centisome position: 20.6

GC content: 49.49

Gene sequence:

>2340_bases
ATGACAACAAACAGCGCGATTGCCGCGTGTGGCCGGGCGGAGGCGACAAGATTCCGTCCGGCAGAAGCGAACGTTCCCTT
TTCCTTACGGAAAAGGATCGTCAGGAGACGCGGAAAACGATCAGCAGGTTGCCACATCCGTGATGGGCCGTGTGTGGATG
ACTTGCACAACACACAAAGAAACATTCCAACAACAATCCGTTTAATCATGAAAAGCAATTTTGTAAAGCCTTCGAAGTCA
TTCATCCGCATGGCAGGCCTGCTTGCCGTGCTCTCGACAGTCTGTCGTCCGGACGCTGCCGCAAAAGAAGTTGCCAAAAC
CGATTCGGCTGACTACGTAGCCGATGAAATCGTCGTCAGCTCAACCAGAACAGACGAAAAACTCAAGAACATCCCGCGCA
AGGTCGAGGTGATTACCAGCAAGGATATTGAGGCGCTCGATCCCGATAATGCCACCGAGTTGCTGCAAAAAACTGCCGGT
GTTGATGTGATCGAGTATCCGGGCGTGCTTTCTGGCGTATCGATGAGGGGCTTCGTGCCAAATTACGGTTCATATCTCAA
TCCCCAGTACGTTACCTATCTCCTTGATGGCCGCCCTCTTGGTACGTACAATCTTGCCTCGGTAGATATGAACATGATCG
AGCGGGTCGAGGTGATTAAAGGCCCATCATCGGCATTGTATGGATCGAAAGGAATGGGAGGCACCATCAACTTTATTACA
AAGAAATCCCGTGGCCCGATCAAGGGGACGGCCTCGCTCGGCTACGGAAGTTTCGAAACCTTCGAGGGCAATGCCGCCGT
CGGCGGTTCGATCAGTGACCGGTTCGATTTCGATATCGGTTTCCGCTACTTCAATCAGGGCGAAGACTACAAGGTTGGAA
AAAATACGCTCATATCGAATCCCGATCCGCAGATTCTCGAAAGGGACATTGATACGATGCACAACAGTACCTACTCGACC
AATTCGGGCATGATGCGTGTCGGTTATCGGTTGAACGACAATTTCAGAGTCGATTTGCGCGGTGCGTTTTTCAATGCCCC
ATCCGTACATACCCCCGGCTCTATCTGGGGCTACTATGGTGAGGGTATGAAAGATGTGTATCGAAAAACAGCAGATCTTT
CGCTTACCGGTACGGCAGGACGTCACAGCATCAAATTCATGCCGTACTGGTCAAAAGACGAATCGAACAATCTCAAGCAA
ACCGAAGCTACACAGAATAAACCGAGTAAAATCTACCCGTATTACCTGGGAGATTTTGAGGAGTATGGCTTTCAGTTGCA
GGATGTTATTGCTTTGGGCAACCACCGTATAACCGGCGGTTTGGATTATGATAACCAGACGTACAAAACCAGGCGGTACA
GTGCACCGGATGTTGCCCAAAGACCGTATAGCGCTGACAGCAGAACTAGTGATTTTGGCCTGTTTACCCAAGCCGCGCTC
TCTTTTCTTGATAATCGCCTGATTGTCACTCCCGGAGTCCGATTCGACGCAACGACATTCGGTTTGCTTGATACACCGCT
TGTTCCCAATGTGAACACCGAAAAAGAGCATGACGGGTTTTTCAGTCCATCGCTGGCATTCCAGTACTCTTTTGTTCCCG
AATTGAAAGTGCATTCGAGTATCGGACGAGCATTTGTCGCGCCTTCGGGGTTGCAGAAAGCTGGTGAATATGTTGACTCT
TTTGGAAGAACGGTCAGGGGTAATCCCGATCTGGAGCCGGAAACAAGCCGAACGTGGGATGTCGGTCTGACCTGGAGTGA
CACGAAAAAAGGAGTGCGGGCCGATGTGACCTACTATGATACTGATTTTAAAGATTTTATTACGCAAGTACAGAGAACGG
ATGGCAGCACGACTTACATGACTTATGTCAATGCGGGAAGCGCGAAGATCAGGGGACTTGAGTTCGAGCTGTCCTACGAC
TTTGGAGCTTTGGCTGATTACCGCTACTCATTACGCTGCTTCGTAAACTATACGCATCAATTTGAGAATGATGTCACAAT
GGGTGGTGTTACGAGTCCAATGAAGTATGTGCGGGACGGTCTTGGTTCATTCGGCATCGAATATGACGATTTCCGGCTAC
TCAGTGCGCGACTCAGTGGTCGCTATCTGGGTACCAGTTATGAAGACAACTATTATCGATCGTATGGACGGTTACCGAAT
GTGTTAGTGATTAAAAACGAACCTGCATTAGTGTTTGATGCGACGGTTGGCATAAAGATCAACGCTCAGAACCGCGTCGA
TCTGATGGTCAAGAATCTTCTCGATGAAAACTATGCCGAAAAAACCGGCTACAACATGCCGGGCCGCTGGTACGGCATGA
AATACATCGTCACCTTCTGA

Upstream 100 bases:

>100_bases
ACTGGTTCGCGCCCGCGAACCGGGAAGGCCGGAAGCGAGGGGAGAGTCAGAAGACCTGCCGTAATGCAGTAAATGCTCCG
GGAAGAGAGCGAATACGAAC

Downstream 100 bases:

>100_bases
GCCGGGCGCAGCAGATGATGGCATTCAATCTCATCAAAAAGGCAGTACTGGTGACGGTACTGCCTCTTCTGCTTCTGACG
GTCTGCGGTTTTGAGTAAGC

Product: receptor, putative

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 779; Mature: 778

Protein sequence:

>779_residues
MTTNSAIAACGRAEATRFRPAEANVPFSLRKRIVRRRGKRSAGCHIRDGPCVDDLHNTQRNIPTTIRLIMKSNFVKPSKS
FIRMAGLLAVLSTVCRPDAAAKEVAKTDSADYVADEIVVSSTRTDEKLKNIPRKVEVITSKDIEALDPDNATELLQKTAG
VDVIEYPGVLSGVSMRGFVPNYGSYLNPQYVTYLLDGRPLGTYNLASVDMNMIERVEVIKGPSSALYGSKGMGGTINFIT
KKSRGPIKGTASLGYGSFETFEGNAAVGGSISDRFDFDIGFRYFNQGEDYKVGKNTLISNPDPQILERDIDTMHNSTYST
NSGMMRVGYRLNDNFRVDLRGAFFNAPSVHTPGSIWGYYGEGMKDVYRKTADLSLTGTAGRHSIKFMPYWSKDESNNLKQ
TEATQNKPSKIYPYYLGDFEEYGFQLQDVIALGNHRITGGLDYDNQTYKTRRYSAPDVAQRPYSADSRTSDFGLFTQAAL
SFLDNRLIVTPGVRFDATTFGLLDTPLVPNVNTEKEHDGFFSPSLAFQYSFVPELKVHSSIGRAFVAPSGLQKAGEYVDS
FGRTVRGNPDLEPETSRTWDVGLTWSDTKKGVRADVTYYDTDFKDFITQVQRTDGSTTYMTYVNAGSAKIRGLEFELSYD
FGALADYRYSLRCFVNYTHQFENDVTMGGVTSPMKYVRDGLGSFGIEYDDFRLLSARLSGRYLGTSYEDNYYRSYGRLPN
VLVIKNEPALVFDATVGIKINAQNRVDLMVKNLLDENYAEKTGYNMPGRWYGMKYIVTF

Sequences:

>Translated_779_residues
MTTNSAIAACGRAEATRFRPAEANVPFSLRKRIVRRRGKRSAGCHIRDGPCVDDLHNTQRNIPTTIRLIMKSNFVKPSKS
FIRMAGLLAVLSTVCRPDAAAKEVAKTDSADYVADEIVVSSTRTDEKLKNIPRKVEVITSKDIEALDPDNATELLQKTAG
VDVIEYPGVLSGVSMRGFVPNYGSYLNPQYVTYLLDGRPLGTYNLASVDMNMIERVEVIKGPSSALYGSKGMGGTINFIT
KKSRGPIKGTASLGYGSFETFEGNAAVGGSISDRFDFDIGFRYFNQGEDYKVGKNTLISNPDPQILERDIDTMHNSTYST
NSGMMRVGYRLNDNFRVDLRGAFFNAPSVHTPGSIWGYYGEGMKDVYRKTADLSLTGTAGRHSIKFMPYWSKDESNNLKQ
TEATQNKPSKIYPYYLGDFEEYGFQLQDVIALGNHRITGGLDYDNQTYKTRRYSAPDVAQRPYSADSRTSDFGLFTQAAL
SFLDNRLIVTPGVRFDATTFGLLDTPLVPNVNTEKEHDGFFSPSLAFQYSFVPELKVHSSIGRAFVAPSGLQKAGEYVDS
FGRTVRGNPDLEPETSRTWDVGLTWSDTKKGVRADVTYYDTDFKDFITQVQRTDGSTTYMTYVNAGSAKIRGLEFELSYD
FGALADYRYSLRCFVNYTHQFENDVTMGGVTSPMKYVRDGLGSFGIEYDDFRLLSARLSGRYLGTSYEDNYYRSYGRLPN
VLVIKNEPALVFDATVGIKINAQNRVDLMVKNLLDENYAEKTGYNMPGRWYGMKYIVTF
>Mature_778_residues
TTNSAIAACGRAEATRFRPAEANVPFSLRKRIVRRRGKRSAGCHIRDGPCVDDLHNTQRNIPTTIRLIMKSNFVKPSKSF
IRMAGLLAVLSTVCRPDAAAKEVAKTDSADYVADEIVVSSTRTDEKLKNIPRKVEVITSKDIEALDPDNATELLQKTAGV
DVIEYPGVLSGVSMRGFVPNYGSYLNPQYVTYLLDGRPLGTYNLASVDMNMIERVEVIKGPSSALYGSKGMGGTINFITK
KSRGPIKGTASLGYGSFETFEGNAAVGGSISDRFDFDIGFRYFNQGEDYKVGKNTLISNPDPQILERDIDTMHNSTYSTN
SGMMRVGYRLNDNFRVDLRGAFFNAPSVHTPGSIWGYYGEGMKDVYRKTADLSLTGTAGRHSIKFMPYWSKDESNNLKQT
EATQNKPSKIYPYYLGDFEEYGFQLQDVIALGNHRITGGLDYDNQTYKTRRYSAPDVAQRPYSADSRTSDFGLFTQAALS
FLDNRLIVTPGVRFDATTFGLLDTPLVPNVNTEKEHDGFFSPSLAFQYSFVPELKVHSSIGRAFVAPSGLQKAGEYVDSF
GRTVRGNPDLEPETSRTWDVGLTWSDTKKGVRADVTYYDTDFKDFITQVQRTDGSTTYMTYVNAGSAKIRGLEFELSYDF
GALADYRYSLRCFVNYTHQFENDVTMGGVTSPMKYVRDGLGSFGIEYDDFRLLSARLSGRYLGTSYEDNYYRSYGRLPNV
LVIKNEPALVFDATVGIKINAQNRVDLMVKNLLDENYAEKTGYNMPGRWYGMKYIVTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=715, Percent_Identity=22.2377622377622, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788478, Length=182, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786344, Length=161, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 87043; Mature: 86912

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNSAIAACGRAEATRFRPAEANVPFSLRKRIVRRRGKRSAGCHIRDGPCVDDLHNTQR
CCCCCHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHC
NIPTTIRLIMKSNFVKPSKSFIRMAGLLAVLSTVCRPDAAAKEVAKTDSADYVADEIVVS
CCCHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHEEE
STRTDEKLKNIPRKVEVITSKDIEALDPDNATELLQKTAGVDVIEYPGVLSGVSMRGFVP
CCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
NYGSYLNPQYVTYLLDGRPLGTYNLASVDMNMIERVEVIKGPSSALYGSKGMGGTINFIT
CCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEE
KKSRGPIKGTASLGYGSFETFEGNAAVGGSISDRFDFDIGFRYFNQGEDYKVGKNTLISN
ECCCCCCCEEEECCCCCCEEECCCEEECCCCCCCCCCEECEEEECCCCCEECCCCCCCCC
PDPQILERDIDTMHNSTYSTNSGMMRVGYRLNDNFRVDLRGAFFNAPSVHTPGSIWGYYG
CCHHHHHHHHHHHCCCCEECCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCC
EGMKDVYRKTADLSLTGTAGRHSIKFMPYWSKDESNNLKQTEATQNKPSKIYPYYLGDFE
CCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCEEEEEEECCHH
EYGFQLQDVIALGNHRITGGLDYDNQTYKTRRYSAPDVAQRPYSADSRTSDFGLFTQAAL
HHCCCHHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHH
SFLDNRLIVTPGVRFDATTFGLLDTPLVPNVNTEKEHDGFFSPSLAFQYSFVPELKVHSS
HHHCCCEEECCCCEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
IGRAFVAPSGLQKAGEYVDSFGRTVRGNPDLEPETSRTWDVGLTWSDTKKGVRADVTYYD
CCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEC
TDFKDFITQVQRTDGSTTYMTYVNAGSAKIRGLEFELSYDFGALADYRYSLRCFVNYTHQ
CCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEECCCCCHHCCEEEEEEEEEEHHH
FENDVTMGGVTSPMKYVRDGLGSFGIEYDDFRLLSARLSGRYLGTSYEDNYYRSYGRLPN
CCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHEEHHHCCEEECCCCCCCHHHHHCCCCC
VLVIKNEPALVFDATVGIKINAQNRVDLMVKNLLDENYAEKTGYNMPGRWYGMKYIVTF
EEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
TTNSAIAACGRAEATRFRPAEANVPFSLRKRIVRRRGKRSAGCHIRDGPCVDDLHNTQR
CCCCHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHC
NIPTTIRLIMKSNFVKPSKSFIRMAGLLAVLSTVCRPDAAAKEVAKTDSADYVADEIVVS
CCCHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHEEE
STRTDEKLKNIPRKVEVITSKDIEALDPDNATELLQKTAGVDVIEYPGVLSGVSMRGFVP
CCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
NYGSYLNPQYVTYLLDGRPLGTYNLASVDMNMIERVEVIKGPSSALYGSKGMGGTINFIT
CCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEE
KKSRGPIKGTASLGYGSFETFEGNAAVGGSISDRFDFDIGFRYFNQGEDYKVGKNTLISN
ECCCCCCCEEEECCCCCCEEECCCEEECCCCCCCCCCEECEEEECCCCCEECCCCCCCCC
PDPQILERDIDTMHNSTYSTNSGMMRVGYRLNDNFRVDLRGAFFNAPSVHTPGSIWGYYG
CCHHHHHHHHHHHCCCCEECCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCEEECCC
EGMKDVYRKTADLSLTGTAGRHSIKFMPYWSKDESNNLKQTEATQNKPSKIYPYYLGDFE
CCHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCEEEEEEECCHH
EYGFQLQDVIALGNHRITGGLDYDNQTYKTRRYSAPDVAQRPYSADSRTSDFGLFTQAAL
HHCCCHHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHCCCCCCCCCCCCHHHHHHHHH
SFLDNRLIVTPGVRFDATTFGLLDTPLVPNVNTEKEHDGFFSPSLAFQYSFVPELKVHSS
HHHCCCEEECCCCEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
IGRAFVAPSGLQKAGEYVDSFGRTVRGNPDLEPETSRTWDVGLTWSDTKKGVRADVTYYD
CCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEC
TDFKDFITQVQRTDGSTTYMTYVNAGSAKIRGLEFELSYDFGALADYRYSLRCFVNYTHQ
CCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEECCCCCHHCCEEEEEEEEEEHHH
FENDVTMGGVTSPMKYVRDGLGSFGIEYDDFRLLSARLSGRYLGTSYEDNYYRSYGRLPN
CCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHEEHHHCCEEECCCCCCCHHHHHCCCCC
VLVIKNEPALVFDATVGIKINAQNRVDLMVKNLLDENYAEKTGYNMPGRWYGMKYIVTF
EEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]