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The map label for this gene is cbiCH [H]

Identifier: 21673226

GI number: 21673226

Start: 401818

End: 403257

Strand: Reverse

Name: cbiCH [H]

Synonym: CT0387

Alternate gene names: 21673226

Gene position: 403257-401818 (Counterclockwise)

Preceding gene: 21673227

Following gene: 21673225

Centisome position: 18.71

GC content: 65.97

Gene sequence:

>1440_bases
TTGCCGGCAGTCAACCCTTTCGCCCTTTGCGTCATGAACGGCACGATTACCGTTGTGGGCCTCGGCCCCGGCAGCGACTC
GATGATGACCCCGCAGGTGCTCGATGCGATCCGCACGGCTGACGCGGTGGTCGGCTACACCGGCTACATGAAGAGCATCG
CACACCTGATTCCGGACTCCGTCCAGACCGTGGCGACTGGCATGACCGGCGAGATGCAGCGGGCCGAAGAGGCGTTCGCG
CTGGCCGCCGAAGGGCGTCACGTCGTTGTCGTCAGCTCCGGCGACGCGGGCATCTACGGCATGGCACCACTCATTGTCGA
ACTGCATGCGAGCGGCCGCTGGCCGGAGGTCGCGGTCGAGGTACTGCCGGGCATCAGTGCGTTCCAGGCCGCTGCCGCGC
GACTCGGCGCTCCGGTGAGCCACGACTTCTGTGCCATTTCGCTCTCCGACCTGATGACGCCGTGGGAGGTGATCGAAAAG
CGGATCGAGGCAGCGGCATCGGCGGACTTCGTGACGGCGGTTTACAACCCACGCAGCCGCGACCGCTACTGGCAGATTCA
CCGCTTCCGCGAGCTGTTCCTGCGCCACCGCTCACCCTCGACGCCGGTGGGCATCGTCCGCAATGTGACGCGCAAGGATG
AGTCGGTCGTGCTGACGACGCTCGGTGAGTTCGATCCCGATTCGCTCGACATGTTCACCATGATGCTGGTTGGCAACTCG
ACCACCTTCTTCTCCGGAGAGCGGATGGTGACGCCGAGGGGCTATTTCAGCCGAGAGGAGCGTGGCCTGAGACCGGTTGG
CCAGTCGATCATGAGCAGCAGCTTCCGCGCCATCGCGGAACTGATGAAGCCGGACTCACGCCCGACTGACGAGCGCTGGG
CGGTCATGCACACGATTCACACGACCGCCGATTTTGAGATGCAGGAGTTGTTCCACGCCACACCCGGCGCAATCCAGCGG
TGGCACGAGGCGCTGACCGCCGGTGGCGCGACGATCATTACCGACGTGACGATGGTGCAGTCGGGGCTGCGCAAGGCAGC
GCTGGAGCGCTACGGCGTGACGGTGCGCTGCTACTTGCACGACGAGCGCGTGACGGAGCTGGCCAAAAGCGCGGGCATCA
CGCGCAGCCAGGCGGGGATGCGGCTTGCCGCCAAGGAGCATCCCGACGCGCTCTTCGTCGTCGGCAATGCGCCGACGGCG
CTGCTGGAACTTGCATCGCTCCTGCATCGCGGAGGCTTCGCTCCGATGGGGGTGATTGGAGCGCCGGTCGGGTTCGTGAA
CGTAGTCGAGTCGAAGCACCGGCTCAAGGCGGCGGCGGGCGCAACGCCGATTGCCGTCATCGAGGGGCGCAAGGGTGGCA
GCGCGATTGCGGCCACCATCGTCAACGCGGCCTTCAGCCTCGACGAGGCGGAGGCGATGAATCCGGGGCGCGATGTCTGA

Upstream 100 bases:

>100_bases
CCGAAAAGGTGGGCATGGCGGGCGAGTTCATCACGATGGAAGTCGACGCCTTGCGCTCACGCGCTATCCCCTACTTTTCG
CTGCTGGTCTGCTCGCCGCA

Downstream 100 bases:

>100_bases
GCAGTTCACGCTCATCGGCCTCAGCGACAGCACGGAGCCGCATCTCGACCCCTCCGCCATCGAGGCAATTCGTGCACATC
GAATCTTCGCAGGCGGAACC

Product: precorrin-3B C17-methyltransferase/precorrin-8X methylmutase

Products: hydrogenobyrinate

Alternate protein names: Cobalt-precorrin-3B C(17)-methyltransferase; Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-8X methylmutase; Cobalt-precorrin isomerase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPAVNPFALCVMNGTITVVGLGPGSDSMMTPQVLDAIRTADAVVGYTGYMKSIAHLIPDSVQTVATGMTGEMQRAEEAFA
LAAEGRHVVVVSSGDAGIYGMAPLIVELHASGRWPEVAVEVLPGISAFQAAAARLGAPVSHDFCAISLSDLMTPWEVIEK
RIEAAASADFVTAVYNPRSRDRYWQIHRFRELFLRHRSPSTPVGIVRNVTRKDESVVLTTLGEFDPDSLDMFTMMLVGNS
TTFFSGERMVTPRGYFSREERGLRPVGQSIMSSSFRAIAELMKPDSRPTDERWAVMHTIHTTADFEMQELFHATPGAIQR
WHEALTAGGATIITDVTMVQSGLRKAALERYGVTVRCYLHDERVTELAKSAGITRSQAGMRLAAKEHPDALFVVGNAPTA
LLELASLLHRGGFAPMGVIGAPVGFVNVVESKHRLKAAAGATPIAVIEGRKGGSAIAATIVNAAFSLDEAEAMNPGRDV

Sequences:

>Translated_479_residues
MPAVNPFALCVMNGTITVVGLGPGSDSMMTPQVLDAIRTADAVVGYTGYMKSIAHLIPDSVQTVATGMTGEMQRAEEAFA
LAAEGRHVVVVSSGDAGIYGMAPLIVELHASGRWPEVAVEVLPGISAFQAAAARLGAPVSHDFCAISLSDLMTPWEVIEK
RIEAAASADFVTAVYNPRSRDRYWQIHRFRELFLRHRSPSTPVGIVRNVTRKDESVVLTTLGEFDPDSLDMFTMMLVGNS
TTFFSGERMVTPRGYFSREERGLRPVGQSIMSSSFRAIAELMKPDSRPTDERWAVMHTIHTTADFEMQELFHATPGAIQR
WHEALTAGGATIITDVTMVQSGLRKAALERYGVTVRCYLHDERVTELAKSAGITRSQAGMRLAAKEHPDALFVVGNAPTA
LLELASLLHRGGFAPMGVIGAPVGFVNVVESKHRLKAAAGATPIAVIEGRKGGSAIAATIVNAAFSLDEAEAMNPGRDV
>Mature_478_residues
PAVNPFALCVMNGTITVVGLGPGSDSMMTPQVLDAIRTADAVVGYTGYMKSIAHLIPDSVQTVATGMTGEMQRAEEAFAL
AAEGRHVVVVSSGDAGIYGMAPLIVELHASGRWPEVAVEVLPGISAFQAAAARLGAPVSHDFCAISLSDLMTPWEVIEKR
IEAAASADFVTAVYNPRSRDRYWQIHRFRELFLRHRSPSTPVGIVRNVTRKDESVVLTTLGEFDPDSLDMFTMMLVGNST
TFFSGERMVTPRGYFSREERGLRPVGQSIMSSSFRAIAELMKPDSRPTDERWAVMHTIHTTADFEMQELFHATPGAIQRW
HEALTAGGATIITDVTMVQSGLRKAALERYGVTVRCYLHDERVTELAKSAGITRSQAGMRLAAKEHPDALFVVGNAPTAL
LELASLLHRGGFAPMGVIGAPVGFVNVVESKHRLKAAAGATPIAVIEGRKGGSAIAATIVNAAFSLDEAEAMNPGRDV

Specific function: Bifunctional enzyme with a methyltransferase domain that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 and an isomerase domain that catalyzes the conversion of cobalt-precorrin-8X to cobyrinic acid; a methyl migration

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CobH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014422
- InterPro:   IPR003722
- InterPro:   IPR006363 [H]

Pfam domain/function: PF02570 CbiC; PF00590 TP_methylase [H]

EC number: 5.4.1.2; 2.1.1.131

Molecular weight: Translated: 51491; Mature: 51359

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVNPFALCVMNGTITVVGLGPGSDSMMTPQVLDAIRTADAVVGYTGYMKSIAHLIPDS
CCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VQTVATGMTGEMQRAEEAFALAAEGRHVVVVSSGDAGIYGMAPLIVELHASGRWPEVAVE
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCEEEEEEECCCCCHHHHHH
VLPGISAFQAAAARLGAPVSHDFCAISLSDLMTPWEVIEKRIEAAASADFVTAVYNPRSR
HHCCHHHHHHHHHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCC
DRYWQIHRFRELFLRHRSPSTPVGIVRNVTRKDESVVLTTLGEFDPDSLDMFTMMLVGNS
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHEEEEEECCC
TTFFSGERMVTPRGYFSREERGLRPVGQSIMSSSFRAIAELMKPDSRPTDERWAVMHTIH
CEEECCCEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TTADFEMQELFHATPGAIQRWHEALTAGGATIITDVTMVQSGLRKAALERYGVTVRCYLH
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEC
DERVTELAKSAGITRSQAGMRLAAKEHPDALFVVGNAPTALLELASLLHRGGFAPMGVIG
HHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHH
APVGFVNVVESKHRLKAAAGATPIAVIEGRKGGSAIAATIVNAAFSLDEAEAMNPGRDV
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCCC
>Mature Secondary Structure 
PAVNPFALCVMNGTITVVGLGPGSDSMMTPQVLDAIRTADAVVGYTGYMKSIAHLIPDS
CCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VQTVATGMTGEMQRAEEAFALAAEGRHVVVVSSGDAGIYGMAPLIVELHASGRWPEVAVE
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCEEEEEEECCCCCHHHHHH
VLPGISAFQAAAARLGAPVSHDFCAISLSDLMTPWEVIEKRIEAAASADFVTAVYNPRSR
HHCCHHHHHHHHHHHCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCC
DRYWQIHRFRELFLRHRSPSTPVGIVRNVTRKDESVVLTTLGEFDPDSLDMFTMMLVGNS
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHEEEEEECCC
TTFFSGERMVTPRGYFSREERGLRPVGQSIMSSSFRAIAELMKPDSRPTDERWAVMHTIH
CEEECCCEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEE
TTADFEMQELFHATPGAIQRWHEALTAGGATIITDVTMVQSGLRKAALERYGVTVRCYLH
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEC
DERVTELAKSAGITRSQAGMRLAAKEHPDALFVVGNAPTALLELASLLHRGGFAPMGVIG
HHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHH
APVGFVNVVESKHRLKAAAGATPIAVIEGRKGGSAIAATIVNAAFSLDEAEAMNPGRDV
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 8X

Specific reaction: precorrin-8X = hydrogenobyrinate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]