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The map label for this gene is purH [H]

Identifier: 21673159

GI number: 21673159

Start: 335653

End: 337224

Strand: Direct

Name: purH [H]

Synonym: CT0320

Alternate gene names: 21673159

Gene position: 335653-337224 (Clockwise)

Preceding gene: 21673154

Following gene: 21673162

Centisome position: 15.58

GC content: 59.54

Gene sequence:

>1572_bases
ATGCTTGATCCTGTCATCAAACGGGCGCTCGTGTCCGTTTCCGACAAAACCGGCATCGTCGATTTCTGCCGCGAACTGGC
CTCGATGGATGTCGAAATCTTCTCCACCGGTGGCACGCTGAAATTACTTCAAGGTGCTGGTATCGTCGCGCAATCCATTT
CGACCATCACCGGTTTTCCGGAGATCATGGACGGCAGGGTCAAGACGCTTCATCCGAAAATCCACGGTGGTCTGCTTGCC
GTGCGCGACAACGCCGAGCACCAGAAGGCGGCCCGCGAGAACGGTATCCAGTTCATCGATCTCGTGGTGGTGAACCTCTA
TCCCTTCGAGGCAACTATCGCCAAAGCGGATGTGACTTTCGAGGAGGCTATTGAAAATATCGATATTGGTGGCCCATCGA
TGCTGCGCAGCGCGGCCAAGAACAACGAGTCGGTCACGGTGGTTACCGATGTCGCCGACTACGCCACCGTGCTTGACGAA
ATGCGCTCGAACGGTGGCGCGACCACGCGGGCCACCCGTCTGACGCTCGCGGCCAAAGTGTACGCGCTGACCTCGCGCTA
CGACACGGCCATTGCGGCCTACATGGCTAAAGCCGCTGGAGTCAAGGGCGCGGGCGACACGATGACCCTCAAGCTCGAAA
AAGAGCTGAGCATGCGCTACGGCGAGAACCCGCACCAGAGCGCCGGACTCTACAAAATGGACGATGGCAACGGCGTGCGT
TCGTTCAGCGCGATTTTCGAGAAGCTGCACGGTAAAGAGTTGTCGTATAACAATATGCTTGACATCGCCGCTGCGACTGG
TATCATCGAGGAGTTTCGCGGCGAGGAGCCGTCGGTGGTGATTGTCAAGCACACCAATCCGTGCGGCGTCGCGCAGGCGC
CGACGCTCTGCGAGGCCTACCGCAAAGCCTTCTCGACCGACACGCAGGCTCCGTTTGGCGGTATCATCGCCTTCAACCGC
CCGCTCGACATGGAGACAGCCAGCGCGGTCAACGAAATCTTCACCGAGATTCTCATCGCTCCGGCTTTCGAAGATGGCGT
GCTCGAGATGCTCATGAAGAAGAAGGACCGCCGTCTCGTGCTCCAGAAGCAGCCGTTGCCGAAAGCTGGCTGGGAGTTCA
AGTCCACGCCGTTCGGCATGCTCGTGCAGGAGCGCGACAGTAAGACGGTTGCTCCTGAAGAGCTGAAGGTGGTCACGAAG
CGCCAGCCGACCGCTGAAGAGCTGGCCGACCTGATGTTCGCCTGGAAGATCGTGCGGCACATCAAATCCAACACGATCCT
CTACGTCAAGAACCGCCAGACCTTCGGCGTCGGCGCGGGCCAGATGTCGCGCGTCGATTCATCGAAAATCGCCCGCTGGA
AAGCCTCGGAAGTGGCCCTCGATCTCAGGGGATCGGTTGTCGCCTCCGACGCCTTCTTCCCCTTCGCCGATGGCCTTCTC
GCTGCCGCTGAAGCTGGGGTAACCGCCGTCATCCAGCCGGGTGGTTCGATCCGCGACAACGAAGTCATCGAAGCCGCCGA
CGCCAACAATCTCGCAATGGTCTTCACCGGAATGCGCCATTTCAAGCACTGA

Upstream 100 bases:

>100_bases
GTGCCTGCGCGGAGTTGCAGTTCTCCGGTCGGTATAGTCGCGGATTTTTGTAAATTCCCGGTCTATTTTTGATTTCCGAA
CACAACACAACTCTCTGCTC

Downstream 100 bases:

>100_bases
GGTAGCAGAAAAAGACAGAGAACTGCAAGGGCAGCAGGAACAACGTGATGAGCGTTGTTCCTGCTGCCTTTTTGTTTTTA
CTGCTATTCCGGGATTGTAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MLDPVIKRALVSVSDKTGIVDFCRELASMDVEIFSTGGTLKLLQGAGIVAQSISTITGFPEIMDGRVKTLHPKIHGGLLA
VRDNAEHQKAARENGIQFIDLVVVNLYPFEATIAKADVTFEEAIENIDIGGPSMLRSAAKNNESVTVVTDVADYATVLDE
MRSNGGATTRATRLTLAAKVYALTSRYDTAIAAYMAKAAGVKGAGDTMTLKLEKELSMRYGENPHQSAGLYKMDDGNGVR
SFSAIFEKLHGKELSYNNMLDIAAATGIIEEFRGEEPSVVIVKHTNPCGVAQAPTLCEAYRKAFSTDTQAPFGGIIAFNR
PLDMETASAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVLQKQPLPKAGWEFKSTPFGMLVQERDSKTVAPEELKVVTK
RQPTAEELADLMFAWKIVRHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVALDLRGSVVASDAFFPFADGLL
AAAEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH

Sequences:

>Translated_523_residues
MLDPVIKRALVSVSDKTGIVDFCRELASMDVEIFSTGGTLKLLQGAGIVAQSISTITGFPEIMDGRVKTLHPKIHGGLLA
VRDNAEHQKAARENGIQFIDLVVVNLYPFEATIAKADVTFEEAIENIDIGGPSMLRSAAKNNESVTVVTDVADYATVLDE
MRSNGGATTRATRLTLAAKVYALTSRYDTAIAAYMAKAAGVKGAGDTMTLKLEKELSMRYGENPHQSAGLYKMDDGNGVR
SFSAIFEKLHGKELSYNNMLDIAAATGIIEEFRGEEPSVVIVKHTNPCGVAQAPTLCEAYRKAFSTDTQAPFGGIIAFNR
PLDMETASAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVLQKQPLPKAGWEFKSTPFGMLVQERDSKTVAPEELKVVTK
RQPTAEELADLMFAWKIVRHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVALDLRGSVVASDAFFPFADGLL
AAAEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
>Mature_523_residues
MLDPVIKRALVSVSDKTGIVDFCRELASMDVEIFSTGGTLKLLQGAGIVAQSISTITGFPEIMDGRVKTLHPKIHGGLLA
VRDNAEHQKAARENGIQFIDLVVVNLYPFEATIAKADVTFEEAIENIDIGGPSMLRSAAKNNESVTVVTDVADYATVLDE
MRSNGGATTRATRLTLAAKVYALTSRYDTAIAAYMAKAAGVKGAGDTMTLKLEKELSMRYGENPHQSAGLYKMDDGNGVR
SFSAIFEKLHGKELSYNNMLDIAAATGIIEEFRGEEPSVVIVKHTNPCGVAQAPTLCEAYRKAFSTDTQAPFGGIIAFNR
PLDMETASAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVLQKQPLPKAGWEFKSTPFGMLVQERDSKTVAPEELKVVTK
RQPTAEELADLMFAWKIVRHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVALDLRGSVVASDAFFPFADGLL
AAAEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=480, Percent_Identity=35.625, Blast_Score=249, Evalue=5e-66,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=49.9054820415879, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71985564, Length=613, Percent_Identity=31.810766721044, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71985574, Length=352, Percent_Identity=25.5681818181818, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323768, Length=476, Percent_Identity=34.0336134453782, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6323056, Length=475, Percent_Identity=32.4210526315789, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24649832, Length=487, Percent_Identity=34.7022587268994, Blast_Score=258, Evalue=1e-68,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56744; Mature: 56744

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPVIKRALVSVSDKTGIVDFCRELASMDVEIFSTGGTLKLLQGAGIVAQSISTITGFP
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHCCCH
EIMDGRVKTLHPKIHGGLLAVRDNAEHQKAARENGIQFIDLVVVNLYPFEATIAKADVTF
HHHCCCCEEECHHHCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEEECCHHEEEECCCCH
EEAIENIDIGGPSMLRSAAKNNESVTVVTDVADYATVLDEMRSNGGATTRATRLTLAAKV
HHHHHHCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YALTSRYDTAIAAYMAKAAGVKGAGDTMTLKLEKELSMRYGENPHQSAGLYKMDDGNGVR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCEEEECCCCCHH
SFSAIFEKLHGKELSYNNMLDIAAATGIIEEFRGEEPSVVIVKHTNPCGVAQAPTLCEAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHH
RKAFSTDTQAPFGGIIAFNRPLDMETASAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLV
HHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
LQKQPLPKAGWEFKSTPFGMLVQERDSKTVAPEELKVVTKRQPTAEELADLMFAWKIVRH
EECCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVALDLRGSVVASDAFFPFADGLL
HCCCEEEEEECCCEECCCCCHHHHCCHHHHHHEEHHHEEEECCCCEEECCCCCHHHHHHH
AAAEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
HHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MLDPVIKRALVSVSDKTGIVDFCRELASMDVEIFSTGGTLKLLQGAGIVAQSISTITGFP
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHCCCH
EIMDGRVKTLHPKIHGGLLAVRDNAEHQKAARENGIQFIDLVVVNLYPFEATIAKADVTF
HHHCCCCEEECHHHCCCEEEEECCCHHHHHHHHCCCEEEEEEEEEEECCHHEEEECCCCH
EEAIENIDIGGPSMLRSAAKNNESVTVVTDVADYATVLDEMRSNGGATTRATRLTLAAKV
HHHHHHCCCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YALTSRYDTAIAAYMAKAAGVKGAGDTMTLKLEKELSMRYGENPHQSAGLYKMDDGNGVR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCEEEECCCCCHH
SFSAIFEKLHGKELSYNNMLDIAAATGIIEEFRGEEPSVVIVKHTNPCGVAQAPTLCEAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHH
RKAFSTDTQAPFGGIIAFNRPLDMETASAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLV
HHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
LQKQPLPKAGWEFKSTPFGMLVQERDSKTVAPEELKVVTKRQPTAEELADLMFAWKIVRH
EECCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVALDLRGSVVASDAFFPFADGLL
HCCCEEEEEECCCEECCCCCHHHHCCHHHHHHEEHHHEEEECCCCEEECCCCCHHHHHHH
AAAEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
HHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA