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The map label for this gene is purF [H]

Identifier: 21673152

GI number: 21673152

Start: 328856

End: 330349

Strand: Reverse

Name: purF [H]

Synonym: CT0313

Alternate gene names: 21673152

Gene position: 330349-328856 (Counterclockwise)

Preceding gene: 21673155

Following gene: 21673143

Centisome position: 15.33

GC content: 56.49

Gene sequence:

>1494_bases
ATGTGCGGAGTTTTCGGCGTTTTTAATTCAAAAACTCCCGCCGAAGATACCTTTTACGGCCTCTACTCGCTACAACACCG
AGGACAGGAAGCCGCAGGTATCGTCGTTGCCGAATACAACAAGGCCAAGAAAAAGACCCTGTTCAAGCAGCATAAAGGGC
TGGGGCTGGTATCCGAAGTTTTCAAGGACGAACAGATTTTCGAGAACCTGAGCGGATACGCAGCAATCGGCCATAACCGC
TACTCCACCACCGGCGCCTCCAAATCGAACAACAATATCCAGCCCTTCTCCCTCACCTACCGCTCCGGCAGCCTCGCCAT
CGCCCACAACGGAAACCTGACCAATTCGAGAGTTTTGCGCAAGGAGCTGACCGAAGAGGGCGTAATTTTCCAGGCCTCAT
CGGATACCGAAATCATCCCGCACCTTGCCGCGCGCAGCAAGGAAAAAGAGCCACTGCACCAGATTTACGATGCATTGCGG
CAGGTAGAAGGAGCTTTTTCGATCGTGATTCTGGCCAACAACCAGATGATTGCGGCGCGAGACCCTTACGGCGTGCGCCC
CTTGGCTCTCGGCAAGAAAATCGATCCCGCAACCGGTGAGCTGGCCTACGTCGTAGCGAGCGAAACCTGTGCTTTCGACA
TCATCAAGGCCGAGTACATCCGCGACATCGAACCGGGTGAAATCCTCCTGATCGACCACCTCGCCGTGGACAACGAAAAG
CCGGTTTCGCTCTACCTTCCACCTGTCGAGCGCAAAGCCCGCTGCATCTTCGAGTATGTCTATTTCGCCCGCCCGGACAG
CTTCATCTTTCGGCACTCGGTTGACAAGGTGCGCCGGAACCTCGGCAAGAACCTGGCCAGGGAATCAACGATCGAACGAG
ACCCCAACGACAAGGAGCTGGCCGTAGTCAGCGTGCCAGACTCCTCGAACACGGCTGCGCTCGGCTTCGTCAGAGAAAGC
AACAAACTCGGCAAGCCTGCGCGCTTCGAGCACGGCCTGATCCGCAACCACTATGTTGGCCGCACCTTTATTCAGCCAGG
CATTCAGAGCCGTGAAATCAAGGTTCGCTCGAAATACAACATCGTGCGCGGCGTCATGCAGGGCCGCCCAATCATCCTCG
TCGATGACTCGATCGTGCGCGGCACAACGGCCAAGATGCTCATCAAGCTGGTTCGCGAGGCCAATCCGAAAGAGATTCAC
CTGCACATCAGCTCACCACCGATCACCAATCCCTGCTTTTACGGCATGGACTTCCCCTCCAAGCGCCAACTGCTCACGCA
CATGCTTGCTGACGCCGAGCACGAGCTGGGCGATATCGAAAAAATCAGGGAGTACATCGGCGTGGATTCACTGAGATATC
TCTCCATGCAGGGCCTCCTGAACAGCGTACCGGAATTCGAGGGCGAAACCTGCAGCTACTGCACTGCCTGCTTCAGCGGC
GACTACCCCATCCCGATCGCCGACGCCACGACCGACAAGGAAGAGAACGACTAA

Upstream 100 bases:

>100_bases
CCCAATTTCTCTTTCATGGTTTTTATGTTTCTCCTTGATATATTAGTTTCACTTGCCTCTCAGAGGCAAAGATTTTACAC
AATCTCAAGATTTTACTGAT

Downstream 100 bases:

>100_bases
GCCTTCCTGTTTGCTGAACGAGAAACCGCGCCAGATAAAAAAGCCGTCTTCGCTTCCGGACAAGACGGCTTTTCTTTTTT
TATGGAAAAAACGAAAGGCT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MCGVFGVFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGLGLVSEVFKDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIEPGEILLIDHLAVDNEK
PVSLYLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEHELGDIEKIREYIGVDSLRYLSMQGLLNSVPEFEGETCSYCTACFSG
DYPIPIADATTDKEEND

Sequences:

>Translated_497_residues
MCGVFGVFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGLGLVSEVFKDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIEPGEILLIDHLAVDNEK
PVSLYLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEHELGDIEKIREYIGVDSLRYLSMQGLLNSVPEFEGETCSYCTACFSG
DYPIPIADATTDKEEND
>Mature_497_residues
MCGVFGVFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGLGLVSEVFKDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIEPGEILLIDHLAVDNEK
PVSLYLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEHELGDIEKIREYIGVDSLRYLSMQGLLNSVPEFEGETCSYCTACFSG
DYPIPIADATTDKEEND

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=530, Percent_Identity=36.9811320754717, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI4826742, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=37.6237623762376, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790167, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=505, Percent_Identity=36.2376237623762, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17532897, Length=217, Percent_Identity=26.2672811059908, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=217, Percent_Identity=26.2672811059908, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539970, Length=217, Percent_Identity=25.3456221198157, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=32.20675944334, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24659598, Length=508, Percent_Identity=38.9763779527559, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24659604, Length=484, Percent_Identity=39.8760330578512, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI28573187, Length=519, Percent_Identity=37.1868978805395, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI21357745, Length=185, Percent_Identity=27.5675675675676, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55516; Mature: 55516

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVFGVFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGLGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHHCCCCHHHHH
FKDEQIFENLSGYAAIGHNRYSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
KELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALRQVEGAFSIVILANNQMIAAR
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIEPGEILLIDHLAVDNEK
CCCCCCCHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
PVSLYLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKEL
CEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVVSVPDSSNTAALGFVRESNKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYN
EEEECCCCCCCEEEEEEECCCCCCCCCHHCCCCHHHCCCCHHHHCCCCCCCEEEEHHHHH
IVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIHLHISSPPITNPCFYGMDFPS
HHHHHHCCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMLADAEHELGDIEKIREYIGVDSLRYLSMQGLLNSVPEFEGETCSYCTACFSG
HHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
DYPIPIADATTDKEEND
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGVFGVFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGLGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHHCCCCHHHHH
FKDEQIFENLSGYAAIGHNRYSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
KELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALRQVEGAFSIVILANNQMIAAR
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIEPGEILLIDHLAVDNEK
CCCCCCCHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
PVSLYLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKEL
CEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVVSVPDSSNTAALGFVRESNKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYN
EEEECCCCCCCEEEEEEECCCCCCCCCHHCCCCHHHCCCCHHHHCCCCCCCEEEEHHHHH
IVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIHLHISSPPITNPCFYGMDFPS
HHHHHHCCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMLADAEHELGDIEKIREYIGVDSLRYLSMQGLLNSVPEFEGETCSYCTACFSG
HHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
DYPIPIADATTDKEEND
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]