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The map label for this gene is gltX [H]

Identifier: 21673138

GI number: 21673138

Start: 310224

End: 311735

Strand: Direct

Name: gltX [H]

Synonym: CT0299

Alternate gene names: 21673138

Gene position: 310224-311735 (Clockwise)

Preceding gene: 21673137

Following gene: 21673139

Centisome position: 14.4

GC content: 57.94

Gene sequence:

>1512_bases
ATGGCCGGTCAAAAGGTTAGAACGCGATTTGCCCCATCTCCAACGGGATACCTCCATGTCGGCGGTCTTCGCACTGCACT
GTACAACTATCTGTTCGCCAAGAGAATGAACGGGGATTTCGTTATCAGGATCGAGGATACCGACCAGAGCCGCAAGGTCG
AGGATGCTGAAAAGAAACTCATCAGTACGCTCGAATGGGCTGGCATCATCGCTGATGAAAGCCCGATGCACGGCGGCAAC
TATGGCCCGTACGTGCAGTCGCAGCGTCTCTCCATCTATCGTGACTACTGTACGCGTCTGCTCGAAGACAAAAACGCCTA
CTACTGCTTCTCGACCCCCGAGGAGCTCGAAGAGAACCGCCAGCTTCAGCTCAAACAGGGGCTTCAGCCCAAGTACAATC
GCAAGTGGCTGCCCGAGGACATGGGTGGCAACATGCCGGAATCCGAAATCAAAAAGAAGCTCGACGAGGGCGCGCCTTAC
GTGGTGAGGATGAAGGTACCGGACTACGTTTCGGTCTGGTTCGAGGATATGATTCGCGGCCCGATCGAGTTCGATTCCGC
CACCATCGACGACCAGGTGTTGATGAAGTCCGATGGTTTTCCGACCTACCACTTTGCCAGCGTCATCGACGATCACCTGA
TGGAGTTCACCCACATCATTCGTGGCGAGGAGTGGCTGCCTTCGATGCCGAAGCACCTGTTGCTCTACGAATTCTTTGGC
TGGGAGCCGCCGAAGTTTGCGCACCTGCCGCTTTTGCTCAATCCCGACCGCTCCAAGCTCAGCAAGCGCCAGGGCGACGT
GGCCGTGGAGGATTACATGCGCAAGGGCTACAGCAGCGAGGCGATTGTCAACTTCGTGGCGCTGCTTGGCTGGAACGAGG
GCGAGGGGAGCGAGCAGGAGGTGTTCAGCATGGAGGAGCTGATCTCGAAGTTTTCGCTCGAACGGGTCGGCAAGGCTGGC
GCTGTGTTCAACGTCGAAAAGCTCTCGTGGCTGGAGAAGCAGTATATCAAGACCCGGCCTGTTGAAAAGATCGTGGGCAA
CATCAAGCCGGTGCTCCAGGCGAAGCTGGCCGAGTTCTCGCCGGAGATGTCCGTCGAGCGCATCACTTCCGACGACTATC
TTGCCAAGGTGGTCGAGCTGATGCGGGAGCGCGTCAATTTCGAGCACGAGTTCGTGACCTTCAGCAGCTACTTCTTCTTC
GAGCCGGAAAGTTATGAAGAGGAGGCCGTGGCCAAGCGCTGGACGCCCAACGTGCCACCGCTGCTTCAGGAGTTTGCCGA
TCTACTCGAAGCGAACGACGACTTTACCGCCGAAAACATCGAGGCGCAGCTCAAGGCGTTCGTGGCTCCGAAAGGCTTGA
AGCCCGCCGTGCTGATTCATCCGATCAGGATCGCCGTCTCTGGCGTCAGCTTTGGCCCGAGCCTCTACCACATGCTCGAA
GTGCTTGGCAAAGAGGCGGTGCTTCGCCGCATCCGCCGCGCCATCGAGCGCATCGAAGTGCCGGCAGCCTGA

Upstream 100 bases:

>100_bases
TTTCGAAAGCGCTCTTGGAATATCTGCACGTTTAGATTTCCGGAAGATGCGCCTGACGTCACAATCTGCCGAAATTATTT
AACACCTGATCTTTCGTATT

Downstream 100 bases:

>100_bases
TCGTCCGATCCCGGGGCATTCCGGTGCCCCGCGGTCGGAAAAAGTTCTTTTTTGTTTGGCGTTAAAAAGGCAATTGCTAT
ATTCACCTCACTCCAATTGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MAGQKVRTRFAPSPTGYLHVGGLRTALYNYLFAKRMNGDFVIRIEDTDQSRKVEDAEKKLISTLEWAGIIADESPMHGGN
YGPYVQSQRLSIYRDYCTRLLEDKNAYYCFSTPEELEENRQLQLKQGLQPKYNRKWLPEDMGGNMPESEIKKKLDEGAPY
VVRMKVPDYVSVWFEDMIRGPIEFDSATIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYMRKGYSSEAIVNFVALLGWNEGEGSEQEVFSMEELISKFSLERVGKAG
AVFNVEKLSWLEKQYIKTRPVEKIVGNIKPVLQAKLAEFSPEMSVERITSDDYLAKVVELMRERVNFEHEFVTFSSYFFF
EPESYEEEAVAKRWTPNVPPLLQEFADLLEANDDFTAENIEAQLKAFVAPKGLKPAVLIHPIRIAVSGVSFGPSLYHMLE
VLGKEAVLRRIRRAIERIEVPAA

Sequences:

>Translated_503_residues
MAGQKVRTRFAPSPTGYLHVGGLRTALYNYLFAKRMNGDFVIRIEDTDQSRKVEDAEKKLISTLEWAGIIADESPMHGGN
YGPYVQSQRLSIYRDYCTRLLEDKNAYYCFSTPEELEENRQLQLKQGLQPKYNRKWLPEDMGGNMPESEIKKKLDEGAPY
VVRMKVPDYVSVWFEDMIRGPIEFDSATIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYMRKGYSSEAIVNFVALLGWNEGEGSEQEVFSMEELISKFSLERVGKAG
AVFNVEKLSWLEKQYIKTRPVEKIVGNIKPVLQAKLAEFSPEMSVERITSDDYLAKVVELMRERVNFEHEFVTFSSYFFF
EPESYEEEAVAKRWTPNVPPLLQEFADLLEANDDFTAENIEAQLKAFVAPKGLKPAVLIHPIRIAVSGVSFGPSLYHMLE
VLGKEAVLRRIRRAIERIEVPAA
>Mature_502_residues
AGQKVRTRFAPSPTGYLHVGGLRTALYNYLFAKRMNGDFVIRIEDTDQSRKVEDAEKKLISTLEWAGIIADESPMHGGNY
GPYVQSQRLSIYRDYCTRLLEDKNAYYCFSTPEELEENRQLQLKQGLQPKYNRKWLPEDMGGNMPESEIKKKLDEGAPYV
VRMKVPDYVSVWFEDMIRGPIEFDSATIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFGW
EPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYMRKGYSSEAIVNFVALLGWNEGEGSEQEVFSMEELISKFSLERVGKAGA
VFNVEKLSWLEKQYIKTRPVEKIVGNIKPVLQAKLAEFSPEMSVERITSDDYLAKVVELMRERVNFEHEFVTFSSYFFFE
PESYEEEAVAKRWTPNVPPLLQEFADLLEANDDFTAENIEAQLKAFVAPKGLKPAVLIHPIRIAVSGVSFGPSLYHMLEV
LGKEAVLRRIRRAIERIEVPAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=504, Percent_Identity=36.5079365079365, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI62241042, Length=318, Percent_Identity=24.5283018867925, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788743, Length=494, Percent_Identity=35.4251012145749, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI226510925, Length=301, Percent_Identity=30.8970099667774, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786895, Length=399, Percent_Identity=22.5563909774436, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=36.7676767676768, Blast_Score=296, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6324540, Length=514, Percent_Identity=36.7704280155642, Blast_Score=322, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6321192, Length=324, Percent_Identity=26.5432098765432, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=38.7673956262425, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24649466, Length=222, Percent_Identity=27.9279279279279, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864488, Length=312, Percent_Identity=24.6794871794872, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57811; Mature: 57680

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQKVRTRFAPSPTGYLHVGGLRTALYNYLFAKRMNGDFVIRIEDTDQSRKVEDAEKKL
CCCCCCHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
ISTLEWAGIIADESPMHGGNYGPYVQSQRLSIYRDYCTRLLEDKNAYYCFSTPEELEENR
HHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCC
QLQLKQGLQPKYNRKWLPEDMGGNMPESEIKKKLDEGAPYVVRMKVPDYVSVWFEDMIRG
HHHHHCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PIEFDSATIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
CCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHC
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYMRKGYSSEAIVNFVALLGWNEGEGSEQE
CCCCCCCCCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
VFSMEELISKFSLERVGKAGAVFNVEKLSWLEKQYIKTRPVEKIVGNIKPVLQAKLAEFS
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PEMSVERITSDDYLAKVVELMRERVNFEHEFVTFSSYFFFEPESYEEEAVAKRWTPNVPP
CCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCH
LLQEFADLLEANDDFTAENIEAQLKAFVAPKGLKPAVLIHPIRIAVSGVSFGPSLYHMLE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEHEEECCCCCHHHHHHHH
VLGKEAVLRRIRRAIERIEVPAA
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGQKVRTRFAPSPTGYLHVGGLRTALYNYLFAKRMNGDFVIRIEDTDQSRKVEDAEKKL
CCCCCHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
ISTLEWAGIIADESPMHGGNYGPYVQSQRLSIYRDYCTRLLEDKNAYYCFSTPEELEENR
HHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCC
QLQLKQGLQPKYNRKWLPEDMGGNMPESEIKKKLDEGAPYVVRMKVPDYVSVWFEDMIRG
HHHHHCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PIEFDSATIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
CCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHC
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYMRKGYSSEAIVNFVALLGWNEGEGSEQE
CCCCCCCCCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
VFSMEELISKFSLERVGKAGAVFNVEKLSWLEKQYIKTRPVEKIVGNIKPVLQAKLAEFS
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PEMSVERITSDDYLAKVVELMRERVNFEHEFVTFSSYFFFEPESYEEEAVAKRWTPNVPP
CCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCH
LLQEFADLLEANDDFTAENIEAQLKAFVAPKGLKPAVLIHPIRIAVSGVSFGPSLYHMLE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEHEEECCCCCHHHHHHHH
VLGKEAVLRRIRRAIERIEVPAA
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA