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The map label for this gene is pfk-2 [H]

Identifier: 21673089

GI number: 21673089

Start: 259749

End: 260921

Strand: Direct

Name: pfk-2 [H]

Synonym: CT0250

Alternate gene names: 21673089

Gene position: 259749-260921 (Clockwise)

Preceding gene: 21673088

Following gene: 21673097

Centisome position: 12.05

GC content: 55.84

Gene sequence:

>1173_bases
ATGCATATCGGTGTTTTGACCGGCGGCGGCGACGCTCCCGGCATCAACGCGTGCATCAAGACCATCGTGACGATCAGCAC
GGAGAAAGGGTATCGGGTGACCGGTATCCGCAGGGGCTGGAACGGTCTTCTGGCGTTTGATCCCGATGATCCGGCATCCC
GCACGGAGCATATCGTCGATCTTGACTCCGAGCTTGTCCGCAGAATCGATCGTACTGGCGGCACCCTGCTTCACACCAGC
CGCATCAATCCCGGCAACCTCAAAAAGAAAGAAATTCCACCATTTCTTCGCAACTCGCCACACCTGTTGCGCACGGGCTT
GCATCACTCCGGAAACTTTGATCTGACCGATCATGTGTTGCGGAGTATTGATGCGCTTGGCCTGGATGTCATCATTGTCA
TCGGCGGTGACGACACGATCGGCTATGCCGATCACCTGGCCAAAGCTGCTGTCAAGGTGATTGGCGTGCCAAAGACGATG
GACAACGATGTTTATGGCTCGGACTACTGCATCGGGTTTGGCACGGCCGTTACCCGCAGTGTCCAGTACATTCATCAGCT
GCGTACCAGTTCCGGCTCGCACGAGCGAATCACTATTGTCGAGCTGTTCGGTCGTAGCACCGGGGAAACCTGCCTCGTCA
GTGCCTACCTGGCCGGTGTTGACCGCGCCCTCATTCCGGAAGTGCCTTTCGACCCCGAAACTCTCGCCGATTACGTTATT
CAGGACAAAGCAGCCAATCCGAGCCAATATGCCATGATTGCTATCAGTGAAGGTGCGCGGATGATTGGCAGCAAGATGAT
CGAATATTGCGGACGGCGGTACGACGAGGATGGCCATGAGCCTGCCGGAATTGGCCAATTGACCCGTGAAACCATCTCCA
TGCTGACCGGCCAGGATGTCATCTGCCAGCAGCTGGGCTATCTGATGCGTTCAGGTATTCCCGATGCTCTGGATCTGATG
GTCGGCTTCAATTTTGCGCAGCTTGCCGTGGAACTGATTGCAGAAGGCACCTTCGGTGTCATGGTGGCGCTCCAGAAAGG
CATCTACACCTGTCTCCCCCTTGCAGAGGTGTCGTCAAACACCAAGCAGGTCGATATCAGTGAACTGTATGACCCGCGCT
ACTACCGCCCGAAGATGCGAAGCGTCATGGGCAAGCCGATGTTTCTTTATTAG

Upstream 100 bases:

>100_bases
GCTTGCCGCTGGCCGCGATCTCAAAAAAACGAGACGCTCACGAAAGTAAGCCGGTTCTTGCCGTTTCTTTGCACCCCAAC
TCTTTGACGGGATAATTGCC

Downstream 100 bases:

>100_bases
CGGATTACCGATATGGCTTGGAACACTGGAGATTGGGTGGTGGGGCTGTGCCCGGATTGGCCAGATCAGTCGGATATGAC
CAATCCCACCGATTTTTTCA

Product: phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MHIGVLTGGGDAPGINACIKTIVTISTEKGYRVTGIRRGWNGLLAFDPDDPASRTEHIVDLDSELVRRIDRTGGTLLHTS
RINPGNLKKKEIPPFLRNSPHLLRTGLHHSGNFDLTDHVLRSIDALGLDVIIVIGGDDTIGYADHLAKAAVKVIGVPKTM
DNDVYGSDYCIGFGTAVTRSVQYIHQLRTSSGSHERITIVELFGRSTGETCLVSAYLAGVDRALIPEVPFDPETLADYVI
QDKAANPSQYAMIAISEGARMIGSKMIEYCGRRYDEDGHEPAGIGQLTRETISMLTGQDVICQQLGYLMRSGIPDALDLM
VGFNFAQLAVELIAEGTFGVMVALQKGIYTCLPLAEVSSNTKQVDISELYDPRYYRPKMRSVMGKPMFLY

Sequences:

>Translated_390_residues
MHIGVLTGGGDAPGINACIKTIVTISTEKGYRVTGIRRGWNGLLAFDPDDPASRTEHIVDLDSELVRRIDRTGGTLLHTS
RINPGNLKKKEIPPFLRNSPHLLRTGLHHSGNFDLTDHVLRSIDALGLDVIIVIGGDDTIGYADHLAKAAVKVIGVPKTM
DNDVYGSDYCIGFGTAVTRSVQYIHQLRTSSGSHERITIVELFGRSTGETCLVSAYLAGVDRALIPEVPFDPETLADYVI
QDKAANPSQYAMIAISEGARMIGSKMIEYCGRRYDEDGHEPAGIGQLTRETISMLTGQDVICQQLGYLMRSGIPDALDLM
VGFNFAQLAVELIAEGTFGVMVALQKGIYTCLPLAEVSSNTKQVDISELYDPRYYRPKMRSVMGKPMFLY
>Mature_390_residues
MHIGVLTGGGDAPGINACIKTIVTISTEKGYRVTGIRRGWNGLLAFDPDDPASRTEHIVDLDSELVRRIDRTGGTLLHTS
RINPGNLKKKEIPPFLRNSPHLLRTGLHHSGNFDLTDHVLRSIDALGLDVIIVIGGDDTIGYADHLAKAAVKVIGVPKTM
DNDVYGSDYCIGFGTAVTRSVQYIHQLRTSSGSHERITIVELFGRSTGETCLVSAYLAGVDRALIPEVPFDPETLADYVI
QDKAANPSQYAMIAISEGARMIGSKMIEYCGRRYDEDGHEPAGIGQLTRETISMLTGQDVICQQLGYLMRSGIPDALDLM
VGFNFAQLAVELIAEGTFGVMVALQKGIYTCLPLAEVSSNTKQVDISELYDPRYYRPKMRSVMGKPMFLY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=387, Percent_Identity=26.3565891472868, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI266453619, Length=410, Percent_Identity=25.8536585365854, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI266453768, Length=410, Percent_Identity=26.0975609756098, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI266453748, Length=410, Percent_Identity=26.0975609756098, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4505749, Length=410, Percent_Identity=26.0975609756098, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI48762920, Length=380, Percent_Identity=26.0526315789474, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790350, Length=344, Percent_Identity=30.5232558139535, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17558788, Length=364, Percent_Identity=27.1978021978022, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25147584, Length=369, Percent_Identity=23.3062330623306, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323861, Length=283, Percent_Identity=25.7950530035336, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652337, Length=360, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17647809, Length=360, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573326, Length=360, Percent_Identity=24.1666666666667, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 42623; Mature: 42623

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGVLTGGGDAPGINACIKTIVTISTEKGYRVTGIRRGWNGLLAFDPDDPASRTEHIVD
CEEEEEECCCCCCCHHHHHHHHHEEECCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHC
LDSELVRRIDRTGGTLLHTSRINPGNLKKKEIPPFLRNSPHLLRTGLHHSGNFDLTDHVL
CHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHCCCCHHHHHCCCCCCCCCHHHHHH
RSIDALGLDVIIVIGGDDTIGYADHLAKAAVKVIGVPKTMDNDVYGSDYCIGFGTAVTRS
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHH
VQYIHQLRTSSGSHERITIVELFGRSTGETCLVSAYLAGVDRALIPEVPFDPETLADYVI
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHH
QDKAANPSQYAMIAISEGARMIGSKMIEYCGRRYDEDGHEPAGIGQLTRETISMLTGQDV
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHH
ICQQLGYLMRSGIPDALDLMVGFNFAQLAVELIAEGTFGVMVALQKGIYTCLPLAEVSSN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHCCHHHHCCC
TKQVDISELYDPRYYRPKMRSVMGKPMFLY
CCEEEHHHHCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MHIGVLTGGGDAPGINACIKTIVTISTEKGYRVTGIRRGWNGLLAFDPDDPASRTEHIVD
CEEEEEECCCCCCCHHHHHHHHHEEECCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHC
LDSELVRRIDRTGGTLLHTSRINPGNLKKKEIPPFLRNSPHLLRTGLHHSGNFDLTDHVL
CHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHCCCCHHHHHCCCCCCCCCHHHHHH
RSIDALGLDVIIVIGGDDTIGYADHLAKAAVKVIGVPKTMDNDVYGSDYCIGFGTAVTRS
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHH
VQYIHQLRTSSGSHERITIVELFGRSTGETCLVSAYLAGVDRALIPEVPFDPETLADYVI
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHH
QDKAANPSQYAMIAISEGARMIGSKMIEYCGRRYDEDGHEPAGIGQLTRETISMLTGQDV
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHH
ICQQLGYLMRSGIPDALDLMVGFNFAQLAVELIAEGTFGVMVALQKGIYTCLPLAEVSSN
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHCCHHHHCCC
TKQVDISELYDPRYYRPKMRSVMGKPMFLY
CCEEEHHHHCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]