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The map label for this gene is dnaB [H]

Identifier: 21673046

GI number: 21673046

Start: 218141

End: 219676

Strand: Reverse

Name: dnaB [H]

Synonym: CT0205

Alternate gene names: 21673046

Gene position: 219676-218141 (Counterclockwise)

Preceding gene: 21673047

Following gene: 21673045

Centisome position: 10.19

GC content: 60.09

Gene sequence:

>1536_bases
ATGAAGCCGCGCGAACCGCAACTCGATTTGAGCCGTGACATCGACTTCAGCAAGGAGAGCCGGGTGCCCCCCTACTCGCT
GGAGGTCGAACAGGAGGTGCTTGCCTGCATCCTGCTCGAAGACGATCCGATCGAACAGGTGATCCAGATTTTCGGCGACA
ACGGCGAAGCGGTCTTTTACGAAAAGCGCCACCAGATCATCTACAAGGCGATGATGCTGCTCTACCAGAAGCGGCAGGCC
ATCGACCTCATCACGGTAAGCGAAGAGCTGTCGCGCATAGGCGAGCTGGAGAACATCGGCGGACGCCCCTACCTCGGCGA
ACTTTCGGGAAAGGTGATCAGCTCGGCGAACATCGAGTTCTACGCCCGGCTGGTGAAGGAGAAATACCTCTACCGGCGGT
TGATCTCGATTTCGTCGCACATTTCGAGCGCAGCCTACAACACCTCGATGGACATCTTCGATCTGGTCGAAAACGCCTCG
CAGCAGTTCTTCAACATCTCGCAGGCGGGTGTCAAGAAAAAGGCCACCAGCATCAAGGAGTTGCTCAAAACCGCCACGAG
AATGATCGAGAATCTCGGCTCGTCACACTCGTCGGTCACGGGCATCGGATCCGGATTTTCGGAACTCGACGAGTACACGG
CGGGCTTCCAGCCGTCGGACATGATTATCATCGCCGCCCGTCCGTCCGCCGGTAAAACCGCCTTCGCGCTGGCACTGGCG
CGAAACGCGGCGGTGAACTTCAACACGCCGGTGCTCTTCTTCAGCCTCGAAATGGCGGAGATTCAGCTCGCGCTCAGGCT
GATGTGCGCCGAAGCCTACGTCGAGTCGCAGCTCGTGAGGCGCGGCCAGATTTCGCCGGAGATGATGAACAAGATCATCA
ACAGCATGGACGCGCTGGCCGACGCCAAGCTCTTCATCGACGACACGCCGGGCATCTCGATCATGGAGCTGACCGCCAAG
ACCCGCCGCATGAAGCAGGAGCATGGCATCGGCATGGTGGTGGTTGACTACCTCCAGCTCGTCACGCCGGTCAGGGACGG
GCGCTCGAACCGCGAGCAGGAGATCGCGCAGATTTCTCGCTCGCTCAAGGCGCTCGCCAAGGAGCTGAACATTCCGGTGA
TCGCGCTGGCGCAGCTCAACCGCTCGGTCGAACAGCGCTCCGGCGACCGCCGGCCTCAGCTCAGCGACCTTCGCGAATCC
GGCTCGATCGAGCAGGACGCCGATATGGTGATATTCCTGTCGAGACCGGAGATGTACGGCATCAAGAACTTCGAGGATGG
CTCCTCGACCAAGGACATAACCGAGGTGGTCATCGGCAAGCAGCGAAACGGCCCGATCGGCACGGTCAGGCTGCTTTTCC
TGAAAAAGTACGGGCGCTTCCAGTCCACGGCCAACAGCTACCTGACAGCCGCCGATAGCGGACAGCAGCAGCCAGAACCC
GCCCGTGGAGATGGCTATCCGGAGGCATCCCTGCCGGAGCCGCCGCCGCTCGATATGGACAGCTTTATCAACAATTCGGA
CGCCGCACCCTTTTAA

Upstream 100 bases:

>100_bases
TGCTGGGAGGATGTGCAGATGCTCCGGGCGCATTACGACAAGGTCTGCCCGAACGGCTGACCGCACAGGAAACCATGAGA
AGACGAGGAAAAGAAAAGGA

Downstream 100 bases:

>100_bases
CCCTGCCGGTCATGGTTTACAGAAAAGCCCCGAAACATTCCGGCGACGCGCCGGACTCGAGCGCAAACCCTGCCGATCGC
CCTGCTTCGACAGGCAAGGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKPREPQLDLSRDIDFSKESRVPPYSLEVEQEVLACILLEDDPIEQVIQIFGDNGEAVFYEKRHQIIYKAMMLLYQKRQA
IDLITVSEELSRIGELENIGGRPYLGELSGKVISSANIEFYARLVKEKYLYRRLISISSHISSAAYNTSMDIFDLVENAS
QQFFNISQAGVKKKATSIKELLKTATRMIENLGSSHSSVTGIGSGFSELDEYTAGFQPSDMIIIAARPSAGKTAFALALA
RNAAVNFNTPVLFFSLEMAEIQLALRLMCAEAYVESQLVRRGQISPEMMNKIINSMDALADAKLFIDDTPGISIMELTAK
TRRMKQEHGIGMVVVDYLQLVTPVRDGRSNREQEIAQISRSLKALAKELNIPVIALAQLNRSVEQRSGDRRPQLSDLRES
GSIEQDADMVIFLSRPEMYGIKNFEDGSSTKDITEVVIGKQRNGPIGTVRLLFLKKYGRFQSTANSYLTAADSGQQQPEP
ARGDGYPEASLPEPPPLDMDSFINNSDAAPF

Sequences:

>Translated_511_residues
MKPREPQLDLSRDIDFSKESRVPPYSLEVEQEVLACILLEDDPIEQVIQIFGDNGEAVFYEKRHQIIYKAMMLLYQKRQA
IDLITVSEELSRIGELENIGGRPYLGELSGKVISSANIEFYARLVKEKYLYRRLISISSHISSAAYNTSMDIFDLVENAS
QQFFNISQAGVKKKATSIKELLKTATRMIENLGSSHSSVTGIGSGFSELDEYTAGFQPSDMIIIAARPSAGKTAFALALA
RNAAVNFNTPVLFFSLEMAEIQLALRLMCAEAYVESQLVRRGQISPEMMNKIINSMDALADAKLFIDDTPGISIMELTAK
TRRMKQEHGIGMVVVDYLQLVTPVRDGRSNREQEIAQISRSLKALAKELNIPVIALAQLNRSVEQRSGDRRPQLSDLRES
GSIEQDADMVIFLSRPEMYGIKNFEDGSSTKDITEVVIGKQRNGPIGTVRLLFLKKYGRFQSTANSYLTAADSGQQQPEP
ARGDGYPEASLPEPPPLDMDSFINNSDAAPF
>Mature_511_residues
MKPREPQLDLSRDIDFSKESRVPPYSLEVEQEVLACILLEDDPIEQVIQIFGDNGEAVFYEKRHQIIYKAMMLLYQKRQA
IDLITVSEELSRIGELENIGGRPYLGELSGKVISSANIEFYARLVKEKYLYRRLISISSHISSAAYNTSMDIFDLVENAS
QQFFNISQAGVKKKATSIKELLKTATRMIENLGSSHSSVTGIGSGFSELDEYTAGFQPSDMIIIAARPSAGKTAFALALA
RNAAVNFNTPVLFFSLEMAEIQLALRLMCAEAYVESQLVRRGQISPEMMNKIINSMDALADAKLFIDDTPGISIMELTAK
TRRMKQEHGIGMVVVDYLQLVTPVRDGRSNREQEIAQISRSLKALAKELNIPVIALAQLNRSVEQRSGDRRPQLSDLRES
GSIEQDADMVIFLSRPEMYGIKNFEDGSSTKDITEVVIGKQRNGPIGTVRLLFLKKYGRFQSTANSYLTAADSGQQQPEP
ARGDGYPEASLPEPPPLDMDSFINNSDAAPF

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=454, Percent_Identity=44.0528634361233, Blast_Score=357, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56863; Mature: 56863

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPREPQLDLSRDIDFSKESRVPPYSLEVEQEVLACILLEDDPIEQVIQIFGDNGEAVFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHCCCCCEEEH
EKRHQIIYKAMMLLYQKRQAIDLITVSEELSRIGELENIGGRPYLGELSGKVISSANIEF
HHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCHHH
YARLVKEKYLYRRLISISSHISSAAYNTSMDIFDLVENASQQFFNISQAGVKKKATSIKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKTATRMIENLGSSHSSVTGIGSGFSELDEYTAGFQPSDMIIIAARPSAGKTAFALALA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
RNAAVNFNTPVLFFSLEMAEIQLALRLMCAEAYVESQLVRRGQISPEMMNKIINSMDALA
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DAKLFIDDTPGISIMELTAKTRRMKQEHGIGMVVVDYLQLVTPVRDGRSNREQEIAQISR
CCEEEEECCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SLKALAKELNIPVIALAQLNRSVEQRSGDRRPQLSDLRESGSIEQDADMVIFLSRPEMYG
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCC
IKNFEDGSSTKDITEVVIGKQRNGPIGTVRLLFLKKYGRFQSTANSYLTAADSGQQQPEP
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
ARGDGYPEASLPEPPPLDMDSFINNSDAAPF
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKPREPQLDLSRDIDFSKESRVPPYSLEVEQEVLACILLEDDPIEQVIQIFGDNGEAVFY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHCCCCCEEEH
EKRHQIIYKAMMLLYQKRQAIDLITVSEELSRIGELENIGGRPYLGELSGKVISSANIEF
HHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCHHH
YARLVKEKYLYRRLISISSHISSAAYNTSMDIFDLVENASQQFFNISQAGVKKKATSIKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKTATRMIENLGSSHSSVTGIGSGFSELDEYTAGFQPSDMIIIAARPSAGKTAFALALA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
RNAAVNFNTPVLFFSLEMAEIQLALRLMCAEAYVESQLVRRGQISPEMMNKIINSMDALA
HCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DAKLFIDDTPGISIMELTAKTRRMKQEHGIGMVVVDYLQLVTPVRDGRSNREQEIAQISR
CCEEEEECCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SLKALAKELNIPVIALAQLNRSVEQRSGDRRPQLSDLRESGSIEQDADMVIFLSRPEMYG
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCC
IKNFEDGSSTKDITEVVIGKQRNGPIGTVRLLFLKKYGRFQSTANSYLTAADSGQQQPEP
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
ARGDGYPEASLPEPPPLDMDSFINNSDAAPF
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]