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The map label for this gene is ptsI [H]

Identifier: 21673045

GI number: 21673045

Start: 216330

End: 218129

Strand: Reverse

Name: ptsI [H]

Synonym: CT0204

Alternate gene names: 21673045

Gene position: 218129-216330 (Counterclockwise)

Preceding gene: 21673046

Following gene: 21673042

Centisome position: 10.12

GC content: 58.61

Gene sequence:

>1800_bases
ATGGTTTACAGAAAAGCCCCGAAACATTCCGGCGACGCGCCGGACTCGAGCGCAAACCCTGCCGATCGCCCTGCTTCGAC
AGGCAAGGAGCGCCGTTATCAAGGCATCGGCAGCGCGAAGGGATTCGCCATCGGAGAGACCTACGAATTCGTCAGAGAGA
CCATCGAGCACGAAACCGCCGACCTCTCGCCGGAGAACATCGAGGGGGAGATCGAGCGCTTCATGACCGCCCTGCACCGG
TCGGAAAAGGAGCTGAAAAAGATCGAACGGGTGACCACCCGCAAAATCGGGCGCCTCTACTCCGATCTGTTCCAGGCGCA
GATCATGCTGCTCAAGGATCCGGTGCTGACCGGCAACATCACCCGCCGCATCCGCCAGGAGCTGAAGCCCGCGCATATCG
TTATCGAGCAGGAGTTCGGCAAACTTCTCGAACACTTCCTCAACTCCGACGACGTCATCTTCCGCGAGCGGGCCGCCGAC
CTGCACGACCTCAAGGAGCGGATCATCCGAAATCTGCACATTCGCAAGCTGCACTCCTGGGTGCCCGAAGGCTCCATTGT
GGTCTCGCACCACCTCTCGCCGGCGGACATCATTCTCCTCAGCCGCAGCAACATCAAGGGCTTCGCCACTGACACCGGCG
GCAAAACTTCGCACGTCTCGCTGATCTGCAAATCGCTCAACATCCCGATTGTAGTCGGCCTGGGCAACTTTTCGCAGAAG
GCGGTTTCGGGCGAACGCATCATTCTCGACGGCAACGAAGGCCTTGTCATCACTAATCCGCTGGATGAAACTGTCGATAC
CTATCTCAAAAAGCGGGAAGAGGAGAGCAAACGCGAGGCCGATGACTCGATCATGGCTCATCGTCACGCCTTTACCCGTT
GCGGCGTGAGGATTTCAGTCTATTCGAACATCGATTTCAAGGAGGAGATCGAACATCTCGACTCGATGGGGGCCGAAGGC
GTGGGATTGTTCAGAACCGAAAATCTTTTCCTCGACGATCTCAAGCCTCCAAAAGAGGCGGCACAACAGGAGTATTACCG
CAAGATGGGCGAGATGCTCGCGCCGAAGCCGCTGGTCATCCGCCTGTTCGACATCGGCGGCGACAAGCTCATCTACTCGC
CCGTCAAGGAGCCGAACCCGAACCTCGGCTGGCGGGGCGTCAGGATTCTGATCGACGTGCCGGAGATTCTCGACGCGCAG
CTCCAGGCGGTCATCAGGGCCAACATTCACGGCAACATCGACGTGCTGATCCCCATGATTTCGTCGGTCGAGGAGATCAT
GCACATCAAACAGGCAGTCGAGGAGCATTATAAACATATCAGATCACTCACCACCGAGCCGCTGGACAAACCGGGCATCG
GCGCCATGATCGAGGTGCCGGCGGCCGTCGAGCTGATCGACGAAATCACGCAAATCGTCGATTTCGTCAGCATCGGCACC
AACGACCTGACCCAGTACACCCTTGCCGTTGACCGCAACAACCTGATCGTGCAGGATCTGTTCGAGAAATTCCACCCGGC
GGTCATCCGCCAGCTTCACCGCGTCATTTCGACCGCGCAGAAAAACCACTGCCGGGTCTCGCTTTGCGGAGACATGGGTT
CCGACCCCTTGGCCACACCATACCTCATTGGCTGCGGCCTCCGGGAGTTCAGCATCGTCAGCTCTGATATCCCGGCACTC
AAGGCGATGGTAGGTAAATACACGGTCGAGGAGTGCGAAGCGCTCGCCGCCGAATGCCTGAAACTCTCCAGCGCCTCGGC
CATCAAGGCGCACCTCGAAGCGTTCGTCAAGGCGCACTGA

Upstream 100 bases:

>100_bases
GAGATGGCTATCCGGAGGCATCCCTGCCGGAGCCGCCGCCGCTCGATATGGACAGCTTTATCAACAATTCGGACGCCGCA
CCCTTTTAACCCTGCCGGTC

Downstream 100 bases:

>100_bases
ACCTGGCGCCGGATTCCGCCGCCTGCATATCACACGCAAGACCGTAAGAGCTCGATAACAAAAAAGCCGCCTCGGAATTA
TCCGGGCGGCTTTTTGCTGT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MVYRKAPKHSGDAPDSSANPADRPASTGKERRYQGIGSAKGFAIGETYEFVRETIEHETADLSPENIEGEIERFMTALHR
SEKELKKIERVTTRKIGRLYSDLFQAQIMLLKDPVLTGNITRRIRQELKPAHIVIEQEFGKLLEHFLNSDDVIFRERAAD
LHDLKERIIRNLHIRKLHSWVPEGSIVVSHHLSPADIILLSRSNIKGFATDTGGKTSHVSLICKSLNIPIVVGLGNFSQK
AVSGERIILDGNEGLVITNPLDETVDTYLKKREEESKREADDSIMAHRHAFTRCGVRISVYSNIDFKEEIEHLDSMGAEG
VGLFRTENLFLDDLKPPKEAAQQEYYRKMGEMLAPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LQAVIRANIHGNIDVLIPMISSVEEIMHIKQAVEEHYKHIRSLTTEPLDKPGIGAMIEVPAAVELIDEITQIVDFVSIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAVIRQLHRVISTAQKNHCRVSLCGDMGSDPLATPYLIGCGLREFSIVSSDIPAL
KAMVGKYTVEECEALAAECLKLSSASAIKAHLEAFVKAH

Sequences:

>Translated_599_residues
MVYRKAPKHSGDAPDSSANPADRPASTGKERRYQGIGSAKGFAIGETYEFVRETIEHETADLSPENIEGEIERFMTALHR
SEKELKKIERVTTRKIGRLYSDLFQAQIMLLKDPVLTGNITRRIRQELKPAHIVIEQEFGKLLEHFLNSDDVIFRERAAD
LHDLKERIIRNLHIRKLHSWVPEGSIVVSHHLSPADIILLSRSNIKGFATDTGGKTSHVSLICKSLNIPIVVGLGNFSQK
AVSGERIILDGNEGLVITNPLDETVDTYLKKREEESKREADDSIMAHRHAFTRCGVRISVYSNIDFKEEIEHLDSMGAEG
VGLFRTENLFLDDLKPPKEAAQQEYYRKMGEMLAPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LQAVIRANIHGNIDVLIPMISSVEEIMHIKQAVEEHYKHIRSLTTEPLDKPGIGAMIEVPAAVELIDEITQIVDFVSIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAVIRQLHRVISTAQKNHCRVSLCGDMGSDPLATPYLIGCGLREFSIVSSDIPAL
KAMVGKYTVEECEALAAECLKLSSASAIKAHLEAFVKAH
>Mature_599_residues
MVYRKAPKHSGDAPDSSANPADRPASTGKERRYQGIGSAKGFAIGETYEFVRETIEHETADLSPENIEGEIERFMTALHR
SEKELKKIERVTTRKIGRLYSDLFQAQIMLLKDPVLTGNITRRIRQELKPAHIVIEQEFGKLLEHFLNSDDVIFRERAAD
LHDLKERIIRNLHIRKLHSWVPEGSIVVSHHLSPADIILLSRSNIKGFATDTGGKTSHVSLICKSLNIPIVVGLGNFSQK
AVSGERIILDGNEGLVITNPLDETVDTYLKKREEESKREADDSIMAHRHAFTRCGVRISVYSNIDFKEEIEHLDSMGAEG
VGLFRTENLFLDDLKPPKEAAQQEYYRKMGEMLAPKPLVIRLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQ
LQAVIRANIHGNIDVLIPMISSVEEIMHIKQAVEEHYKHIRSLTTEPLDKPGIGAMIEVPAAVELIDEITQIVDFVSIGT
NDLTQYTLAVDRNNLIVQDLFEKFHPAVIRQLHRVISTAQKNHCRVSLCGDMGSDPLATPYLIGCGLREFSIVSSDIPAL
KAMVGKYTVEECEALAAECLKLSSASAIKAHLEAFVKAH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=580, Percent_Identity=32.2413793103448, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1789193, Length=584, Percent_Identity=30.4794520547945, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788726, Length=506, Percent_Identity=31.8181818181818, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI48994992, Length=459, Percent_Identity=32.6797385620915, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1787994, Length=445, Percent_Identity=25.1685393258427, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67007; Mature: 67007

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRKAPKHSGDAPDSSANPADRPASTGKERRYQGIGSAKGFAIGETYEFVRETIEHETA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
DLSPENIEGEIERFMTALHRSEKELKKIERVTTRKIGRLYSDLFQAQIMLLKDPVLTGNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TRRIRQELKPAHIVIEQEFGKLLEHFLNSDDVIFRERAADLHDLKERIIRNLHIRKLHSW
HHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHH
VPEGSIVVSHHLSPADIILLSRSNIKGFATDTGGKTSHVSLICKSLNIPIVVGLGNFSQK
CCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHH
AVSGERIILDGNEGLVITNPLDETVDTYLKKREEESKREADDSIMAHRHAFTRCGVRISV
HCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
YSNIDFKEEIEHLDSMGAEGVGLFRTENLFLDDLKPPKEAAQQEYYRKMGEMLAPKPLVI
ECCCCHHHHHHHHHHCCCCCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQLQAVIRANIHGNIDVLIPMI
EEEECCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHH
SSVEEIMHIKQAVEEHYKHIRSLTTEPLDKPGIGAMIEVPAAVELIDEITQIVDFVSIGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCC
NDLTQYTLAVDRNNLIVQDLFEKFHPAVIRQLHRVISTAQKNHCRVSLCGDMGSDPLATP
CCHHEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
YLIGCGLREFSIVSSDIPALKAMVGKYTVEECEALAAECLKLSSASAIKAHLEAFVKAH
HHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVYRKAPKHSGDAPDSSANPADRPASTGKERRYQGIGSAKGFAIGETYEFVRETIEHETA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
DLSPENIEGEIERFMTALHRSEKELKKIERVTTRKIGRLYSDLFQAQIMLLKDPVLTGNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TRRIRQELKPAHIVIEQEFGKLLEHFLNSDDVIFRERAADLHDLKERIIRNLHIRKLHSW
HHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHH
VPEGSIVVSHHLSPADIILLSRSNIKGFATDTGGKTSHVSLICKSLNIPIVVGLGNFSQK
CCCCCEEEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHH
AVSGERIILDGNEGLVITNPLDETVDTYLKKREEESKREADDSIMAHRHAFTRCGVRISV
HCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
YSNIDFKEEIEHLDSMGAEGVGLFRTENLFLDDLKPPKEAAQQEYYRKMGEMLAPKPLVI
ECCCCHHHHHHHHHHCCCCCCEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RLFDIGGDKLIYSPVKEPNPNLGWRGVRILIDVPEILDAQLQAVIRANIHGNIDVLIPMI
EEEECCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHH
SSVEEIMHIKQAVEEHYKHIRSLTTEPLDKPGIGAMIEVPAAVELIDEITQIVDFVSIGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCC
NDLTQYTLAVDRNNLIVQDLFEKFHPAVIRQLHRVISTAQKNHCRVSLCGDMGSDPLATP
CCHHEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
YLIGCGLREFSIVSSDIPALKAMVGKYTVEECEALAAECLKLSSASAIKAHLEAFVKAH
HHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]