Click here to switch to the map view.

The map label for this gene is thiL [H]

Identifier: 21673040

GI number: 21673040

Start: 210643

End: 211713

Strand: Reverse

Name: thiL [H]

Synonym: CT0199

Alternate gene names: 21673040

Gene position: 211713-210643 (Counterclockwise)

Preceding gene: 21673042

Following gene: 21673037

Centisome position: 9.82

GC content: 60.22

Gene sequence:

>1071_bases
ATGTCATATAACCCTATTTCCGAGATTGGCGAATTCGGTCTGATCGACCGGCTTGCAAAGATTACCGCCCCCTCCACAAA
GCAGCGACCGGAGCTGATCGAAGGGATCGGCGACGACTGCGCGGTGTGGCAAAGCGACGAGTCGGTGGTGCAGGTCGCGA
CGACCGACATTCTGACCGAACACGTCCACTTCGACCTCTTGACCACGCCACTACACCACCTCGGAAGCAAGGCAATCAGC
GTCAACGTCTCGGACATCTGCGCGATGAACGCCATGCCGGATTACGCCGTGATCTCGATCGCCGTACCGCCGAAGATGCC
AGTGGAGATGGTCGAAGAGCTGTACAAGGGAATGAACCACGCCGCCGAAATCTATGGCCTCGCCATCGCGGGCGGCGACA
CCTCGTCGTCGGCGTCGGGGCTGTTCATCTCGGTTTCAATGACCGGAACGACAACGCCGGAGCTGCTCACATTGCGCAAA
GGCGCGTCGCCGGGCGATTTGATCTGTGTGACGGGAACGCTTGGCGGCTCGACCGCCGGCCTGCACCTGTTGCAGCGTGA
AAAGGCGACGATGATCGAGCAGATGCGCAACAACGAACCGTACAACAAGGAGGTGATGGCCGAGTTGCAGGAATACACCG
AAGCAATTCGCTGCCACCTGCTGCCGAAAGCACGCATCGACATCATCGACTTTTTCAGCGAAGAGGGCATCGTACCGACC
GCCATGATCGATATTTCGGACGGCCTGGTCTCCGACCTCGGCCACCTCTGCCGCCGCTCCGGTGTCGGCGCGAAGATCGA
CGAGAGCAAGCTGCCCGTCCTGCCCGAAGCGCGAGCCGTGGCCGAAGAGTTCCAGCAGGACGTCTTCGACTGGGCGCTCA
CCGGCGGCGAAGACTACCAGCTCCTCTTCACCGTCCCGAAATCGCAGTACGACCTCATCGCCAGCCACCGCGACATCACG
GTCATCGGCGAAATCTCCGAGAAAGAAGAGGGCATGATGCTGACCGACATCTTCGGCATGACCATCGACATGGCCGACAT
GAAACGGGGATTCGATCACTTCGCGGGGTGA

Upstream 100 bases:

>100_bases
CTAACTTGTCAAAAAAGCCCATGGAAAATGAGAAATTGGCTATGTTTACAGACGAAGGGATCGGAACGGATTCCGGATTG
AATTCAATGTACGCAAATAT

Downstream 100 bases:

>100_bases
GGGCGGACGACGATCCCTGAAGCTTGTGCATTCCCTTTAAAGATAGTACATTAAACGAGTTCCAACCGGAAAGGTTGCCG
AAGTGGCGGAACTGGTAGAC

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSYNPISEIGEFGLIDRLAKITAPSTKQRPELIEGIGDDCAVWQSDESVVQVATTDILTEHVHFDLLTTPLHHLGSKAIS
VNVSDICAMNAMPDYAVISIAVPPKMPVEMVEELYKGMNHAAEIYGLAIAGGDTSSSASGLFISVSMTGTTTPELLTLRK
GASPGDLICVTGTLGGSTAGLHLLQREKATMIEQMRNNEPYNKEVMAELQEYTEAIRCHLLPKARIDIIDFFSEEGIVPT
AMIDISDGLVSDLGHLCRRSGVGAKIDESKLPVLPEARAVAEEFQQDVFDWALTGGEDYQLLFTVPKSQYDLIASHRDIT
VIGEISEKEEGMMLTDIFGMTIDMADMKRGFDHFAG

Sequences:

>Translated_356_residues
MSYNPISEIGEFGLIDRLAKITAPSTKQRPELIEGIGDDCAVWQSDESVVQVATTDILTEHVHFDLLTTPLHHLGSKAIS
VNVSDICAMNAMPDYAVISIAVPPKMPVEMVEELYKGMNHAAEIYGLAIAGGDTSSSASGLFISVSMTGTTTPELLTLRK
GASPGDLICVTGTLGGSTAGLHLLQREKATMIEQMRNNEPYNKEVMAELQEYTEAIRCHLLPKARIDIIDFFSEEGIVPT
AMIDISDGLVSDLGHLCRRSGVGAKIDESKLPVLPEARAVAEEFQQDVFDWALTGGEDYQLLFTVPKSQYDLIASHRDIT
VIGEISEKEEGMMLTDIFGMTIDMADMKRGFDHFAG
>Mature_355_residues
SYNPISEIGEFGLIDRLAKITAPSTKQRPELIEGIGDDCAVWQSDESVVQVATTDILTEHVHFDLLTTPLHHLGSKAISV
NVSDICAMNAMPDYAVISIAVPPKMPVEMVEELYKGMNHAAEIYGLAIAGGDTSSSASGLFISVSMTGTTTPELLTLRKG
ASPGDLICVTGTLGGSTAGLHLLQREKATMIEQMRNNEPYNKEVMAELQEYTEAIRCHLLPKARIDIIDFFSEEGIVPTA
MIDISDGLVSDLGHLCRRSGVGAKIDESKLPVLPEARAVAEEFQQDVFDWALTGGEDYQLLFTVPKSQYDLIASHRDITV
IGEISEKEEGMMLTDIFGMTIDMADMKRGFDHFAG

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=353, Percent_Identity=32.8611898016997, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 38742; Mature: 38610

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNPISEIGEFGLIDRLAKITAPSTKQRPELIEGIGDDCAVWQSDESVVQVATTDILTE
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEECCCCCEEEHHHHHHHHH
HVHFDLLTTPLHHLGSKAISVNVSDICAMNAMPDYAVISIAVPPKMPVEMVEELYKGMNH
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCH
AAEIYGLAIAGGDTSSSASGLFISVSMTGTTTPELLTLRKGASPGDLICVTGTLGGSTAG
HHHEEEEEEECCCCCCCCCEEEEEEEECCCCCHHHHEEECCCCCCCEEEEEECCCCCHHH
LHLLQREKATMIEQMRNNEPYNKEVMAELQEYTEAIRCHLLPKARIDIIDFFSEEGIVPT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEE
AMIDISDGLVSDLGHLCRRSGVGAKIDESKLPVLPEARAVAEEFQQDVFDWALTGGEDYQ
EEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LLFTVPKSQYDLIASHRDITVIGEISEKEEGMMLTDIFGMTIDMADMKRGFDHFAG
EEEECCCCCHHHHHCCCCEEEEECCCCCCCCEEEEEHHCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
SYNPISEIGEFGLIDRLAKITAPSTKQRPELIEGIGDDCAVWQSDESVVQVATTDILTE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEECCCCCEEEHHHHHHHHH
HVHFDLLTTPLHHLGSKAISVNVSDICAMNAMPDYAVISIAVPPKMPVEMVEELYKGMNH
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCH
AAEIYGLAIAGGDTSSSASGLFISVSMTGTTTPELLTLRKGASPGDLICVTGTLGGSTAG
HHHEEEEEEECCCCCCCCCEEEEEEEECCCCCHHHHEEECCCCCCCEEEEEECCCCCHHH
LHLLQREKATMIEQMRNNEPYNKEVMAELQEYTEAIRCHLLPKARIDIIDFFSEEGIVPT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEE
AMIDISDGLVSDLGHLCRRSGVGAKIDESKLPVLPEARAVAEEFQQDVFDWALTGGEDYQ
EEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LLFTVPKSQYDLIASHRDITVIGEISEKEEGMMLTDIFGMTIDMADMKRGFDHFAG
EEEECCCCCHHHHHCCCCEEEEECCCCCCCCEEEEEHHCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377; 8419296 [H]