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The map label for this gene is trpB-2 [H]

Identifier: 21673033

GI number: 21673033

Start: 202288

End: 203664

Strand: Reverse

Name: trpB-2 [H]

Synonym: CT0192

Alternate gene names: 21673033

Gene position: 203664-202288 (Counterclockwise)

Preceding gene: 21673037

Following gene: 21673031

Centisome position: 9.45

GC content: 64.34

Gene sequence:

>1377_bases
ATGAGTACAGAGCCTACCAAAATCCTCCTGAGCGAGGATGAAATGCCCCGTCAGTGGTACAACATCCAGGCCGACCTGCC
TTCGCCCATGCCGCCGCCGGTGGGGCTGGATGGCAATCCGATCGGGCCGGATGCGCTGGCCAAAGTGTTTCCGATGAACC
TGATCGAGCAGGAGGTGAGCACCGAGCGCTGGATCGATATTCCGGAAGAAATTCTCGGCATCCTCAAGCTGTGGCGGCCT
TCGCCGCTCTACCGCGCGCGGCGACTCGAAGCGGCGCTCGGCACTCCGGCCAAAATCTACTACAAGAACGAAGGGGTCTC
GCCCGCCGGGAGCCACAAGCCCAACACTGCGGTGGCGCAGGCGTGGTACAACCGGGAGTTCGGTATCAAGTACCTCACCA
CCGAAACCGGCGCGGGCCAATGGGGCAGCGCGCTGGCGATGAGCTGCAAGCTGATCGGCATCGAGTGCAAGGTGTTCATG
GTGCGCATCAGCTTCGACCAGAAGCCGTTCCGCAAGATCATGATGAACACCTGGGGCGCGGAGTGCATCCCCAGCCCGAG
CCCGCTGACAGCGGTTGGGCGCAGGATTCTCGAAGAGGATCCCGACACGCCGGGCAGCCTCGGCATCGCCATCAGCGAGG
CAATCGAGCAAGCAGTCGAGCGCGACGACACCCGCTACGCCCTCGGCAGCGTGCTGAATCACGTGATGCTGCACCAAACC
ATCATCGGCCTCGAAGCCAGGAAACAGTTCGACAAGATCGGGCGCTACCCGGACATCGTGATCGGCTGCGCGGGCGGCGG
CTCGAACTTCGCGGGCATCAGCTTCCCGTTCCTGTACGACAAGATTCACGGCAAGGATGTGCAGGTGATCGCAACCGAGC
CGGAGGCGTGCCCGACGCTGACCCGCGCGCCCTACGCCTATGATTCGGGCGACGTGGCGATGATGACGCCGCTCCTGCCG
ATGCACAGCCTCGGCCACACCTTCATTCCGCCGGCCATCCACGCGGGCGGCCTGCGCTACCACGGCATGGCTCCGCTGGT
GAGCCACACCAAGCAGCTCGGGCTGATCGAAGCCACCGCGCTGCCGCAGACCGAGTGCTACGAAGCTGCCCTGCTCTTCG
CCCACACCGAAGGGTTCATCCCCGCGCCGGAGACTTCGCACGCCATCGCGCAGACCATCCGCGAAGCAAAGCAGGCCAAG
GAGGAGGGCAAGGAGAAGGTGATCCTGATGAACTGGTCCGGCCACGGACTGATGGACTTGCAGGGCTACGACGCCTACAT
GTCGGGCAAAATCAGCGACTACCCGCTGCCGGAGGAGCTGCTGCAACGCTCCATCGCCGCCTCGCTCGAAGGCCATCCGC
CGGTGCCGGGCTGCTGA

Upstream 100 bases:

>100_bases
ATCCGCCTGCGCTCTCCCTACCTTTCATGATGGCCACCGGACATCCCGGCCATGAGAGCCGTCGCCGCTCTCTTTTCTCT
TACTTCAAAGCATTACACGC

Downstream 100 bases:

>100_bases
ACAGGCGCAGAAATCGATCATCCATTCAGGAGGGTTTTAACCCTCTGGACATTCCAACCGTTTACATTCATGTTGCCCCG
GCTTGAAAGCCGGGGTAGTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSTEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVSTERWIDIPEEILGILKLWRP
SPLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQAWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDQKPFRKIMMNTWGAECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQT
IIGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTLTRAPYAYDSGDVAMMTPLLP
MHSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAK
EEGKEKVILMNWSGHGLMDLQGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC

Sequences:

>Translated_458_residues
MSTEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVSTERWIDIPEEILGILKLWRP
SPLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQAWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFM
VRISFDQKPFRKIMMNTWGAECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQT
IIGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTLTRAPYAYDSGDVAMMTPLLP
MHSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAK
EEGKEKVILMNWSGHGLMDLQGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC
>Mature_457_residues
STEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVSTERWIDIPEEILGILKLWRPS
PLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQAWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMV
RISFDQKPFRKIMMNTWGAECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQTI
IGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTLTRAPYAYDSGDVAMMTPLLPM
HSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATALPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAKE
EGKEKVILMNWSGHGLMDLQGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=28.0839895013123, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=359, Percent_Identity=30.3621169916435, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50155; Mature: 50024

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVS
CCCCCCEEEECCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TERWIDIPEEILGILKLWRPSPLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQ
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
AWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDQKPFRKIMMNTWGA
HHHCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCC
ECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQT
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IIGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
TRAPYAYDSGDVAMMTPLLPMHSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATA
CCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHCCCEEECCCCHHHHHHHHCCEEEECC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAKEEGKEKVILMNWSGHGLMDL
CCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC
CCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVS
CCCCCEEEECCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TERWIDIPEEILGILKLWRPSPLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQ
HHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHH
AWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDQKPFRKIMMNTWGA
HHHCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCC
ECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQT
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IIGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
TRAPYAYDSGDVAMMTPLLPMHSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATA
CCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHCCCEEECCCCHHHHHHHHCCEEEECC
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAKEEGKEKVILMNWSGHGLMDL
CCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
QGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC
CCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]