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The map label for this gene is glpK [H]

Identifier: 21673026

GI number: 21673026

Start: 194745

End: 196196

Strand: Direct

Name: glpK [H]

Synonym: CT0185

Alternate gene names: 21673026

Gene position: 194745-196196 (Clockwise)

Preceding gene: 21673023

Following gene: 21673027

Centisome position: 9.04

GC content: 65.7

Gene sequence:

>1452_bases
ATGGCTATTCTTTCCATCGATCAGGGCACGACCGGCACCACCTGCATGATCTACGACCGGACGGGCAGCGTGCTCGCCCG
CGCGTATCGCGAGTTGACGCAACACTATCCGCAGGCGGGGTGGGTGGAGCATGATCCGGAGGAGATCTGGCGGACGGTGG
TCGAGTGCGTGGCCGAGGTGCGGGGCGCATATTCGGGTCACATCGAAGCTATCGGCATCACCAACCAGCGCGAGACCACC
GTGGTGTGGGATCGGCGGAGCGGCCAGCCCGTGCACCGCGCCATCGTCTGGCAGTGCCGGCGGACGGCCAAATTGTGCAA
CCGCTACCGCAGCGAGGAGGCGGCGATCCGCGCGAAGACAGGACTGCCGGTGGACGCCTACTTCAGCGCCACCAAGATTC
GCTGGATTCTCGACGCGCATCCCGAAATCGATCCATCAAATCTCCTCTTCGGCACCATTGACACGTGGCTCATCTGGAAG
CTGACCGGCGGCGAGGTTCACGCGACCGACCTGACGAATGCTTCGCGCACGCTGTTGTTTGATATCCACGAGCGGCGCTG
GTCGCCCGAGCTTTGCGAGTTGTTTGGCGTGCCGCTCTCAATGCTACCGGAGGCGCGTCCGTCGATGGGCGGGTTCGGCT
CGGTGCGCACGATTCCGGCGCTCGATGGCGTGCTGATTGCGGGCGTGGCGGGCGACCAGCAGGCGGCGCTTTTTGGCCAG
TGCTGCTTTGCGCCGGGGTCGGTCAAGAACACCTACGGCACCGGCTGCTTCATGGTGATGAACACCGGAGAAAAGTTTGT
GAGTTCGAGTCACGGCTTGTTGACGACGCTCGCGCTCGACGGCGCTGGCCGGAGCTGCTACGCGGTCGAGGGGTCGGTGT
TCATCGGCGGCGCGGTGATGCAGTGGCTGCGCGACGGCTTGCAGCTCATCGGCAGCGCCGCCGAGTCTGAGACGATTGCC
CGCTCGGTTGAATCGAATGGAGGGGTCTATCTCGTGCCCGCCTTCGTCGGTCTCGGCGCACCGCACTGGAACATGGAGGC
GCGGGGCACGATCACCGGCCTGACGCGCGGCTCGTCGCGGGCGCATATTGTCCGCGCGGCGCTGGAGTCGATCGCCTTCC
AGTCGCACGACGTGTTTCGTGCGATGGTGGCCGACATCGGCATTCAGCCGCAGTCGCTCACGGTCGATGGCGGCGCGGTG
AGCAACGAGTTTCTGATGCAGCTCCAGGCCGACCTGCTCGGCGTGCCGGTGCACCGTCCGCGCAACATCGAATCCACTGC
CCTCGGCGCGGCCTGCCTTGCGGGTCTCGAAGCCGGAGTGTGGGGTTCCGCCGCCGAACTGCGTGTGCTCAACAGCGTCG
AGCGGGTCTTCACTCCCGCGATGCCGGAGGGCGAACGCGAGGCGTTGCTGGCGGGCTGGCAAAAGGCGCTGCGGCAGACG
CTCACATCATAA

Upstream 100 bases:

>100_bases
CAACGCGTTGCGCAACTCTCGAATCGTCCGCAGATCGGATTTTTCAGGTGAATCGGTATCTTGCTTTTAGCTGCACGTTG
AACCTTTCCTCGCAAATCCT

Downstream 100 bases:

>100_bases
ACCATTCATCAAAAAGAAGATGACACACAATGATCATCCCGGCGAAGGCACGCCGAAAACCGTCGTTTGCCCCATGTGCG
GCGAGCCATTCACCTGCGGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAILSIDQGTTGTTCMIYDRTGSVLARAYRELTQHYPQAGWVEHDPEEIWRTVVECVAEVRGAYSGHIEAIGITNQRETT
VVWDRRSGQPVHRAIVWQCRRTAKLCNRYRSEEAAIRAKTGLPVDAYFSATKIRWILDAHPEIDPSNLLFGTIDTWLIWK
LTGGEVHATDLTNASRTLLFDIHERRWSPELCELFGVPLSMLPEARPSMGGFGSVRTIPALDGVLIAGVAGDQQAALFGQ
CCFAPGSVKNTYGTGCFMVMNTGEKFVSSSHGLLTTLALDGAGRSCYAVEGSVFIGGAVMQWLRDGLQLIGSAAESETIA
RSVESNGGVYLVPAFVGLGAPHWNMEARGTITGLTRGSSRAHIVRAALESIAFQSHDVFRAMVADIGIQPQSLTVDGGAV
SNEFLMQLQADLLGVPVHRPRNIESTALGAACLAGLEAGVWGSAAELRVLNSVERVFTPAMPEGEREALLAGWQKALRQT
LTS

Sequences:

>Translated_483_residues
MAILSIDQGTTGTTCMIYDRTGSVLARAYRELTQHYPQAGWVEHDPEEIWRTVVECVAEVRGAYSGHIEAIGITNQRETT
VVWDRRSGQPVHRAIVWQCRRTAKLCNRYRSEEAAIRAKTGLPVDAYFSATKIRWILDAHPEIDPSNLLFGTIDTWLIWK
LTGGEVHATDLTNASRTLLFDIHERRWSPELCELFGVPLSMLPEARPSMGGFGSVRTIPALDGVLIAGVAGDQQAALFGQ
CCFAPGSVKNTYGTGCFMVMNTGEKFVSSSHGLLTTLALDGAGRSCYAVEGSVFIGGAVMQWLRDGLQLIGSAAESETIA
RSVESNGGVYLVPAFVGLGAPHWNMEARGTITGLTRGSSRAHIVRAALESIAFQSHDVFRAMVADIGIQPQSLTVDGGAV
SNEFLMQLQADLLGVPVHRPRNIESTALGAACLAGLEAGVWGSAAELRVLNSVERVFTPAMPEGEREALLAGWQKALRQT
LTS
>Mature_482_residues
AILSIDQGTTGTTCMIYDRTGSVLARAYRELTQHYPQAGWVEHDPEEIWRTVVECVAEVRGAYSGHIEAIGITNQRETTV
VWDRRSGQPVHRAIVWQCRRTAKLCNRYRSEEAAIRAKTGLPVDAYFSATKIRWILDAHPEIDPSNLLFGTIDTWLIWKL
TGGEVHATDLTNASRTLLFDIHERRWSPELCELFGVPLSMLPEARPSMGGFGSVRTIPALDGVLIAGVAGDQQAALFGQC
CFAPGSVKNTYGTGCFMVMNTGEKFVSSSHGLLTTLALDGAGRSCYAVEGSVFIGGAVMQWLRDGLQLIGSAAESETIAR
SVESNGGVYLVPAFVGLGAPHWNMEARGTITGLTRGSSRAHIVRAALESIAFQSHDVFRAMVADIGIQPQSLTVDGGAVS
NEFLMQLQADLLGVPVHRPRNIESTALGAACLAGLEAGVWGSAAELRVLNSVERVFTPAMPEGEREALLAGWQKALRQTL
TS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=500, Percent_Identity=45.4, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI4504007, Length=500, Percent_Identity=45.4, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI42794763, Length=505, Percent_Identity=44.950495049505, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=43.687374749499, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=34.2574257425743, Blast_Score=306, Evalue=4e-83,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=51.8145161290323, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789987, Length=497, Percent_Identity=26.3581488933602, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789168, Length=482, Percent_Identity=23.6514522821577, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535599, Length=496, Percent_Identity=41.7338709677419, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=26.295585412668, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=31, Blast_Score=131, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=516, Percent_Identity=35.6589147286822, Blast_Score=288, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=39.9193548387097, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=39.9193548387097, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=39.9193548387097, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=39.9193548387097, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=39.9193548387097, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI17864214, Length=503, Percent_Identity=41.5506958250497, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24654573, Length=459, Percent_Identity=43.1372549019608, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI19922058, Length=528, Percent_Identity=34.469696969697, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=34.2105263157895, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24656367, Length=489, Percent_Identity=30.2658486707566, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=29.9212598425197, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=33.030303030303, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=29.8245614035088, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 52227; Mature: 52096

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILSIDQGTTGTTCMIYDRTGSVLARAYRELTQHYPQAGWVEHDPEEIWRTVVECVAEV
CEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RGAYSGHIEAIGITNQRETTVVWDRRSGQPVHRAIVWQCRRTAKLCNRYRSEEAAIRAKT
HCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEECC
GLPVDAYFSATKIRWILDAHPEIDPSNLLFGTIDTWLIWKLTGGEVHATDLTNASRTLLF
CCCCHHEECCEEEEEEEECCCCCCCCCEEEEECCEEEEEEEECCEEEEEECCCCCCEEEE
DIHERRWSPELCELFGVPLSMLPEARPSMGGFGSVRTIPALDGVLIAGVAGDQQAALFGQ
EEHHHCCCHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
CCFAPGSVKNTYGTGCFMVMNTGEKFVSSSHGLLTTLALDGAGRSCYAVEGSVFIGGAVM
HHCCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEEEEECCCCCEEEEECCCEEECHHHH
QWLRDGLQLIGSAAESETIARSVESNGGVYLVPAFVGLGAPHWNMEARGTITGLTRGSSR
HHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCCEEEEECCCCCH
AHIVRAALESIAFQSHDVFRAMVADIGIQPQSLTVDGGAVSNEFLMQLQADLLGVPVHRP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
RNIESTALGAACLAGLEAGVWGSAAELRVLNSVERVFTPAMPEGEREALLAGWQKALRQT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LTS
HCC
>Mature Secondary Structure 
AILSIDQGTTGTTCMIYDRTGSVLARAYRELTQHYPQAGWVEHDPEEIWRTVVECVAEV
EEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RGAYSGHIEAIGITNQRETTVVWDRRSGQPVHRAIVWQCRRTAKLCNRYRSEEAAIRAKT
HCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEECC
GLPVDAYFSATKIRWILDAHPEIDPSNLLFGTIDTWLIWKLTGGEVHATDLTNASRTLLF
CCCCHHEECCEEEEEEEECCCCCCCCCEEEEECCEEEEEEEECCEEEEEECCCCCCEEEE
DIHERRWSPELCELFGVPLSMLPEARPSMGGFGSVRTIPALDGVLIAGVAGDQQAALFGQ
EEHHHCCCHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
CCFAPGSVKNTYGTGCFMVMNTGEKFVSSSHGLLTTLALDGAGRSCYAVEGSVFIGGAVM
HHCCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEEEEECCCCCEEEEECCCEEECHHHH
QWLRDGLQLIGSAAESETIARSVESNGGVYLVPAFVGLGAPHWNMEARGTITGLTRGSSR
HHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCCEEEEECCCCCH
AHIVRAALESIAFQSHDVFRAMVADIGIQPQSLTVDGGAVSNEFLMQLQADLLGVPVHRP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
RNIESTALGAACLAGLEAGVWGSAAELRVLNSVERVFTPAMPEGEREALLAGWQKALRQT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LTS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA