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The map label for this gene is eno

Identifier: 21672986

GI number: 21672986

Start: 147061

End: 148374

Strand: Direct

Name: eno

Synonym: CT0145

Alternate gene names: 21672986

Gene position: 147061-148374 (Clockwise)

Preceding gene: 21672983

Following gene: 21672987

Centisome position: 6.82

GC content: 58.9

Gene sequence:

>1314_bases
ATGTCAGTCATCACCAGGATTCATGCCCGCCAGATAATGGACTCGCGAGGAAACCCGACGGTCGAGGTGGATGTTCATAC
TGAAAGTTCTTTTGGCCGTGCTGCGGTGCCAAGCGGCGCCTCGACGGGTGTTCACGAAGCGGTCGAGCTGAGGGACAAGG
ACAAGAGCGTTTTTCTCGGCAAAGGGGTGCTCAAGGCTGTCGAGAACGTCAACACCTTGATCAATGATGCTTTGCTGGGC
ATGGACGTGACTGAGCAGGAGGCTATCGACGCGAAGCTCATCGAGCTTGACGGTACGCCGAACAAGTCGAAACTCGGCGC
GAACGCCATTCTTGGCGTTTCACTCGCCTGCGCAAAAGCTGGAGCCGAATATTCAGCCCTGCCGCTCTACCGCTACATCG
GCGGAACGACGGCCAAGACCCTGCCCGTGCCGATGATGAACGTGCTCAACGGTGGCGCTCATGCTGACAATACGGTTGAT
TTCCAGGAGTTCATGATTATGCCGATCGGCTTCGAGCGCTATTCCGATGCGCTTCGGTGCGGAGCCGAGGTGTTTCACTC
GCTCAAGTCCCTGCTACACGATCGCGGTCTGAGCACGGCGGTGGGCGACGAAGGCGGATTTGCGCCGAACGTGGAGTCCA
ACGAACAGGCCATCGAGCTGGTGATCGAGGCCATCGGCATGGCTGGCTACAAAGCTGGTGCGCCGACTGACAGGGGAGGC
CTCGGCGATGGTCATGTCATGATCGCACTCGATCCGGCCAGCTCTGAGTTCTACGACGCCGAAAAGAAAAAGTACGTTTT
CAAGAAATCCTCCGGACGCGAACTTTCGTCAGAAGAGATGGCCAGCTACTGGGCCGACTGGGCGAGCCGCTATCCGATCA
TCTCGATCGAAGATGGCATGGCTGAGGATGACTGGGAAGGCTGGAAGATGCTGACCGACAAGATCGGCGGCCGTGTGCAG
CTTGTGGGTGATGACCTGTTCGTGACCAACAGCAAGCGCCTTGCCGAAGGTATCGAGAAGGGCGTCGGCAACTCGATTCT
CATCAAGGTCAACCAGATCGGCACTCTGACCGAAACCCTTCAGGCCATCGAGCTGGCCAAGCGCAACGGCTACACCTCGG
TCATCAGCCATCGCAGCGGCGAGACCGAAGACACCACCATTGCGCAGATCGCCGTGGCGACCAACGCCGGACAGATCAAG
ACCGGCAGCATGTCGCGCTCCGACCGCATGGCCAAGTACAACGAGCTGCTCAGAATCGAGGAAGAGCTTGGCAGCACGGC
GCTCTATCCGGGCATCGGGGCCTTCCGGGTCTGA

Upstream 100 bases:

>100_bases
CTCTTTTGCCATCGATAAAATGAGTTACGATTTCCGAACAATGAGCTCCATTGCGTATCTTTTGGGTCATGTTCTTCATT
CAATTCTAAATACCCGTATC

Downstream 100 bases:

>100_bases
CGGCGTCGGATTGCTGTTTTTGGAATCATTTCGAGGCGCCATGCCTGGCGATCTGGCAGGCATGGCGCTGAAAACTGCTA
CTGGCCCATGACGAAATACC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSVITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHEAVELRDKDKSVFLGKGVLKAVENVNTLINDALLG
MDVTEQEAIDAKLIELDGTPNKSKLGANAILGVSLACAKAGAEYSALPLYRYIGGTTAKTLPVPMMNVLNGGAHADNTVD
FQEFMIMPIGFERYSDALRCGAEVFHSLKSLLHDRGLSTAVGDEGGFAPNVESNEQAIELVIEAIGMAGYKAGAPTDRGG
LGDGHVMIALDPASSEFYDAEKKKYVFKKSSGRELSSEEMASYWADWASRYPIISIEDGMAEDDWEGWKMLTDKIGGRVQ
LVGDDLFVTNSKRLAEGIEKGVGNSILIKVNQIGTLTETLQAIELAKRNGYTSVISHRSGETEDTTIAQIAVATNAGQIK
TGSMSRSDRMAKYNELLRIEEELGSTALYPGIGAFRV

Sequences:

>Translated_437_residues
MSVITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHEAVELRDKDKSVFLGKGVLKAVENVNTLINDALLG
MDVTEQEAIDAKLIELDGTPNKSKLGANAILGVSLACAKAGAEYSALPLYRYIGGTTAKTLPVPMMNVLNGGAHADNTVD
FQEFMIMPIGFERYSDALRCGAEVFHSLKSLLHDRGLSTAVGDEGGFAPNVESNEQAIELVIEAIGMAGYKAGAPTDRGG
LGDGHVMIALDPASSEFYDAEKKKYVFKKSSGRELSSEEMASYWADWASRYPIISIEDGMAEDDWEGWKMLTDKIGGRVQ
LVGDDLFVTNSKRLAEGIEKGVGNSILIKVNQIGTLTETLQAIELAKRNGYTSVISHRSGETEDTTIAQIAVATNAGQIK
TGSMSRSDRMAKYNELLRIEEELGSTALYPGIGAFRV
>Mature_436_residues
SVITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHEAVELRDKDKSVFLGKGVLKAVENVNTLINDALLGM
DVTEQEAIDAKLIELDGTPNKSKLGANAILGVSLACAKAGAEYSALPLYRYIGGTTAKTLPVPMMNVLNGGAHADNTVDF
QEFMIMPIGFERYSDALRCGAEVFHSLKSLLHDRGLSTAVGDEGGFAPNVESNEQAIELVIEAIGMAGYKAGAPTDRGGL
GDGHVMIALDPASSEFYDAEKKKYVFKKSSGRELSSEEMASYWADWASRYPIISIEDGMAEDDWEGWKMLTDKIGGRVQL
VGDDLFVTNSKRLAEGIEKGVGNSILIKVNQIGTLTETLQAIELAKRNGYTSVISHRSGETEDTTIAQIAVATNAGQIKT
GSMSRSDRMAKYNELLRIEEELGSTALYPGIGAFRV

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=51.9450800915332, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.1739130434783, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=51.2585812356979, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=51.2585812356979, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=45.7471264367816, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=25.6484149855908, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=25.6484149855908, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=25.6484149855908, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1789141, Length=436, Percent_Identity=57.7981651376147, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=53.1963470319635, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=53.1963470319635, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=47.1502590673575, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=49.7727272727273, Blast_Score=408, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=440, Percent_Identity=48.6363636363636, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=440, Percent_Identity=48.6363636363636, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=440, Percent_Identity=48.6363636363636, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=49.3181818181818, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=443, Percent_Identity=50.3386004514673, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO2_CHLTE (Q8KG25)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661051.1
- ProteinModelPortal:   Q8KG25
- SMR:   Q8KG25
- GeneID:   1007040
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0145
- NMPDR:   fig|194439.1.peg.145
- TIGR:   CT0145
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CTEP194439:CT_0145-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46947; Mature: 46815

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 349-349 BINDING 154-154 BINDING 163-163 BINDING 297-297 BINDING 324-324 BINDING 349-349 BINDING 400-400

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHEAVELRDKDKSVFLG
CCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEH
KGVLKAVENVNTLINDALLGMDVTEQEAIDAKLIELDGTPNKSKLGANAILGVSLACAKA
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHC
GAEYSALPLYRYIGGTTAKTLPVPMMNVLNGGAHADNTVDFQEFMIMPIGFERYSDALRC
CCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHHH
GAEVFHSLKSLLHDRGLSTAVGDEGGFAPNVESNEQAIELVIEAIGMAGYKAGAPTDRGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LGDGHVMIALDPASSEFYDAEKKKYVFKKSSGRELSSEEMASYWADWASRYPIISIEDGM
CCCCEEEEEECCCCCCHHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
AEDDWEGWKMLTDKIGGRVQLVGDDLFVTNSKRLAEGIEKGVGNSILIKVNQIGTLTETL
CCCCCHHHHHHHHHCCCEEEEEECCEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHH
QAIELAKRNGYTSVISHRSGETEDTTIAQIAVATNAGQIKTGSMSRSDRMAKYNELLRIE
HHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHH
EELGSTALYPGIGAFRV
HHHCCEEECCCCCCCCC
>Mature Secondary Structure 
SVITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHEAVELRDKDKSVFLG
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEH
KGVLKAVENVNTLINDALLGMDVTEQEAIDAKLIELDGTPNKSKLGANAILGVSLACAKA
HHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHC
GAEYSALPLYRYIGGTTAKTLPVPMMNVLNGGAHADNTVDFQEFMIMPIGFERYSDALRC
CCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHHH
GAEVFHSLKSLLHDRGLSTAVGDEGGFAPNVESNEQAIELVIEAIGMAGYKAGAPTDRGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LGDGHVMIALDPASSEFYDAEKKKYVFKKSSGRELSSEEMASYWADWASRYPIISIEDGM
CCCCEEEEEECCCCCCHHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
AEDDWEGWKMLTDKIGGRVQLVGDDLFVTNSKRLAEGIEKGVGNSILIKVNQIGTLTETL
CCCCCHHHHHHHHHCCCEEEEEECCEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHH
QAIELAKRNGYTSVISHRSGETEDTTIAQIAVATNAGQIKTGSMSRSDRMAKYNELLRIE
HHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHH
EELGSTALYPGIGAFRV
HHHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901