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The map label for this gene is dnaN [H]

Identifier: 21672842

GI number: 21672842

Start: 2

End: 1126

Strand: Reverse

Name: dnaN [H]

Synonym: CT0001

Alternate gene names: 21672842

Gene position: 1126-2 (Counterclockwise)

Preceding gene: 21672843

Following gene: NA

Centisome position: 0.05

GC content: 50.22

Gene sequence:

>1125_bases
ATGAAATTCAATACGACCATCAAACGACTGCAGGAAGCTGTAAACAAGGTTATTCTCGCCGTTCCGGCAAAATCACTCGA
TGCCCGGTTCGACAATATCAACCTGACCCTCGAGAACGGAATGCTTACCATGTTTGCGACTGATGGTGAGCTGTCGATCA
CCACGAACTGCGATGTGGCTTCAACCGACAAGGGCAACATTGCCATCCGAGCCAGAACCTTGCAGGATTTTCTGCGCAGC
ATGTACGAAACTGACGTCACCTTCGACATCGAGCGCCAGTCGATCGGTGAACTCGGCACCATTCATATTGCCACTGACAA
GGGTCGTTATCGCATTCCGTGCACCTTCACCACGAAACCGGAAAGTCAGAAGCGGAATTTCGATCTCTCGATTGAGCTTC
AGCAAAACGAGCTTCAGGACATGATTCATAAAACACTGTTCGCGTGCAGTGTCGATGGCATGCGCCCAGCGATGATGGGT
GTACTGTTCGAATTCGATGAAGAGTATATCACCGCTGTTTCAACCGATGGACATCGACTCGTCCGCTGCCGAAAGAAACC
CGGGGTGACAGTGGCTGAAAAGCAGAAGATCGTCGTGCCGGCAAGGGTACTGTCAATCATTCAGAGAATGCTGACAAACG
AAGAGGTGAAAATGACGATTGACTCAGAGCGTCGGAATGTCAAGTTCAAAACTGCTACCATGGAATTAGAGTCAGCACTC
ATTGTCGAACCGTATCCCAATTACGAGGCGGTGATACCGGTTGAAAACGAAAAGCAGCTTGTGACGGACCGTTCGCAGTT
GCACGATTCGGTTAAAAGGGTCGGACGCTTTTCAAGCATCGGTGACCTGAAGATCAGCGCACAGGATTCACAGATCAAGG
TGATGGCTGAAAACGCCAGTGAGGGTGAATCTGCACAGGAAGAGCTGCCGTGTACGTTCACCGGTGAAGAGATCACGATC
GGTTTTAATGCAAAGTTTGTCGAAGCCGCCCTGTCTCATATCGAAACCGAACAGGTCTCCATCGAAATGAGCAGCCCGAC
AACGGCTGTGTTGTTCAAACCGAAATACGAGGAGCGCCAGGACGATCTGATCATTCTGGTGATGCCGGTCAGAATCAACA
ATTGA

Upstream 100 bases:

>100_bases
AAAATAACATTTTAATGAGTCTCATTGCTCCCCTGTCCAGATTTCCACAAGCTCAACAACAACAACAAATCTTTTATATA
AAAATAAAGATTGTAAGAAG

Downstream 100 bases:

>100_bases
CGGAACAGGAGAGGCTTGCGACTCGACTCCATCAGTATCGCCAATTTCAGGAATCACACGCTTCTTGAATTCGAGCCAGG
CCACTCCATTACCAATATTT

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFNTTIKRLQEAVNKVILAVPAKSLDARFDNINLTLENGMLTMFATDGELSITTNCDVASTDKGNIAIRARTLQDFLRS
MYETDVTFDIERQSIGELGTIHIATDKGRYRIPCTFTTKPESQKRNFDLSIELQQNELQDMIHKTLFACSVDGMRPAMMG
VLFEFDEEYITAVSTDGHRLVRCRKKPGVTVAEKQKIVVPARVLSIIQRMLTNEEVKMTIDSERRNVKFKTATMELESAL
IVEPYPNYEAVIPVENEKQLVTDRSQLHDSVKRVGRFSSIGDLKISAQDSQIKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSIEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN

Sequences:

>Translated_374_residues
MKFNTTIKRLQEAVNKVILAVPAKSLDARFDNINLTLENGMLTMFATDGELSITTNCDVASTDKGNIAIRARTLQDFLRS
MYETDVTFDIERQSIGELGTIHIATDKGRYRIPCTFTTKPESQKRNFDLSIELQQNELQDMIHKTLFACSVDGMRPAMMG
VLFEFDEEYITAVSTDGHRLVRCRKKPGVTVAEKQKIVVPARVLSIIQRMLTNEEVKMTIDSERRNVKFKTATMELESAL
IVEPYPNYEAVIPVENEKQLVTDRSQLHDSVKRVGRFSSIGDLKISAQDSQIKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSIEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN
>Mature_374_residues
MKFNTTIKRLQEAVNKVILAVPAKSLDARFDNINLTLENGMLTMFATDGELSITTNCDVASTDKGNIAIRARTLQDFLRS
MYETDVTFDIERQSIGELGTIHIATDKGRYRIPCTFTTKPESQKRNFDLSIELQQNELQDMIHKTLFACSVDGMRPAMMG
VLFEFDEEYITAVSTDGHRLVRCRKKPGVTVAEKQKIVVPARVLSIIQRMLTNEEVKMTIDSERRNVKFKTATMELESAL
IVEPYPNYEAVIPVENEKQLVTDRSQLHDSVKRVGRFSSIGDLKISAQDSQIKVMAENASEGESAQEELPCTFTGEEITI
GFNAKFVEAALSHIETEQVSIEMSSPTTAVLFKPKYEERQDDLIILVMPVRINN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=24.2105263157895, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42159; Mature: 42159

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNTTIKRLQEAVNKVILAVPAKSLDARFDNINLTLENGMLTMFATDGELSITTNCDVA
CCCCHHHHHHHHHHHHEEEEECCHHHCCEECEEEEEEECCEEEEEEECCEEEEEECCCCC
STDKGNIAIRARTLQDFLRSMYETDVTFDIERQSIGELGTIHIATDKGRYRIPCTFTTKP
CCCCCCEEEEEHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEEEECCCCEEEEEEEECCC
ESQKRNFDLSIELQQNELQDMIHKTLFACSVDGMRPAMMGVLFEFDEEYITAVSTDGHRL
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCEE
VRCRKKPGVTVAEKQKIVVPARVLSIIQRMLTNEEVKMTIDSERRNVKFKTATMELESAL
EEEECCCCCEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCEE
IVEPYPNYEAVIPVENEKQLVTDRSQLHDSVKRVGRFSSIGDLKISAQDSQIKVMAENAS
EECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCC
EGESAQEELPCTFTGEEITIGFNAKFVEAALSHIETEQVSIEMSSPTTAVLFKPKYEERQ
CCCCHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCC
DDLIILVMPVRINN
CCEEEEEEEEEECC
>Mature Secondary Structure
MKFNTTIKRLQEAVNKVILAVPAKSLDARFDNINLTLENGMLTMFATDGELSITTNCDVA
CCCCHHHHHHHHHHHHEEEEECCHHHCCEECEEEEEEECCEEEEEEECCEEEEEECCCCC
STDKGNIAIRARTLQDFLRSMYETDVTFDIERQSIGELGTIHIATDKGRYRIPCTFTTKP
CCCCCCEEEEEHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEEEECCCCEEEEEEEECCC
ESQKRNFDLSIELQQNELQDMIHKTLFACSVDGMRPAMMGVLFEFDEEYITAVSTDGHRL
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCEE
VRCRKKPGVTVAEKQKIVVPARVLSIIQRMLTNEEVKMTIDSERRNVKFKTATMELESAL
EEEECCCCCEEECCCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCEE
IVEPYPNYEAVIPVENEKQLVTDRSQLHDSVKRVGRFSSIGDLKISAQDSQIKVMAENAS
EECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCC
EGESAQEELPCTFTGEEITIGFNAKFVEAALSHIETEQVSIEMSSPTTAVLFKPKYEERQ
CCCCHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCC
DDLIILVMPVRINN
CCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]