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The map label for this gene is hisH [H]

Identifier: 15925665

GI number: 15925665

Start: 2843177

End: 2843755

Strand: Reverse

Name: hisH [H]

Synonym: SAV2675

Alternate gene names: 15925665

Gene position: 2843755-2843177 (Counterclockwise)

Preceding gene: 15925666

Following gene: 15925664

Centisome position: 98.79

GC content: 36.79

Gene sequence:

>579_bases
ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAACATTTAGGGTATGAGGTGGT
TGTCTCAAATACCTCAAAAATAATCGATCAAGCAGAAACAATCATATTGCCCGGTGTCGGCCATTTTAAAGATGCGATGT
CAGAGATAAAACGATTAAATCTCAATGCAATATTGGCTAAGAATACTGATAAGAAGATGATTGGTATTTGTTTAGGCATG
CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGAAATATTTCGCGTATCCAAAC
AGAATACCCAGTGCCGCACTTAGGTTGGAATAATTTAGTGAGTAAGCATCCTATGTTAAATCAAGATGTTTACTTCGTAC
ATTCTTACCAAGCGCCGATGTCAGAAAATGTCATTGCATATGCGCAGTATGGGGCTGATATTCCGGCAATTGTTCAATTT
AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATTTTGCGTCAGGCAATACAAGG
GGGATTTATAAATGATTGA

Upstream 100 bases:

>100_bases
ATGAAATTGAAGCTATATTCAAAGCGTTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGATGATCAGCGTGTGCCG
TCATCGAAAGGTGTGATTGA

Downstream 100 bases:

>100_bases
ATTATGGCCAGCGATTGATTTGATTGGGTCAACAAGTGTGAGGTTAACAGAGGGTAAATATGATAGTGAAGAAAAAATGT
CGCGTTCGGCTGAAGAAAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Sequences:

>Translated_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND
>Mature_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=36.4102564102564, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=32.2115384615385, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21280; Mature: 21280

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
CEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC
>Mature Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
CEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA