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The map label for this gene is hisF

Identifier: 15925663

GI number: 15925663

Start: 2841725

End: 2842483

Strand: Reverse

Name: hisF

Synonym: SAV2673

Alternate gene names: 15925663

Gene position: 2842483-2841725 (Counterclockwise)

Preceding gene: 15925664

Following gene: 15925662

Centisome position: 98.75

GC content: 36.89

Gene sequence:

>759_bases
ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGGATTCAATTTAAAGGATTAAG
GGATATAGGGAATCCTGTTGATTTAGCAATATATTACAATGAAGCGGGTGCTGATGAATTAGTATTTTTAGACATCTCTA
AGACGGAAGAGGGTCATAGCTTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGTTAAATCATGGTGCAGATAAAGTATCATTAAATTCAAGTGCTTT
AAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTCGGTAGACAATGCATCTGTATAGCAATTGATAGCTATTATG
ATCCTGAAAGAAAAGCACATTATTGTTGTACGCATGGTGGTAAAAAAATGACAAATATTAAAGTATATGACTGGGTACAG
CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATGAAACAAGGATTTGATATTGA
GCATCTTGCAAAAATAAAATCCCTTGTAAATATTCCAATCATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAG
AACTATTTAATCAGACGGATGTTTCTGCAGGTTTAGCTGCAAGTATATTACATGACCGAGAAACGACGGTTCAATCAATT
AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA

Upstream 100 bases:

>100_bases
TTAGACATCAGCAAGATATTCAACGATTAGCGTCGCTAAATGTTCACGCTGCTATTATAGGAAAGGCTGCACATCAAGCA
TCTTTTTGGGAGGGGCTAGA

Downstream 100 bases:

>100_bases
CCAAATATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAATCAAACAAAACAAGTATTGATGTTG
GGTTATATGAATCAAGAAGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Sequences:

>Translated_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR
>Mature_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=42.578125, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI87082028, Length=184, Percent_Identity=27.1739130434783, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.2268370607029, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_STAA1 (A7X763)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001443247.1
- ProteinModelPortal:   A7X763
- SMR:   A7X763
- STRING:   A7X763
- EnsemblBacteria:   EBSTAT00000003670
- GeneID:   5560334
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2657
- eggNOG:   COG0107
- GeneTree:   EBGT00050000024240
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   SAUR418127:SAHV_2657-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27530; Mature: 27530

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCCEEECCCCCEECEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC
>Mature Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCCEEECCCCCEECEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA