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The map label for this gene is ydjE [H]

Identifier: 15925030

GI number: 15925030

Start: 2159005

End: 2159964

Strand: Reverse

Name: ydjE [H]

Synonym: SAV2040

Alternate gene names: 15925030

Gene position: 2159964-2159005 (Counterclockwise)

Preceding gene: 15925031

Following gene: 15925025

Centisome position: 75.04

GC content: 34.79

Gene sequence:

>960_bases
ATGAGACGTCTATTTTCAATAGGAGAAGCGTTAATTGATTTTATTCCAAATGTTACAAATGCGAATTTAAAAGATGTTCA
AACATTTACGAAGCAAATTGGTGGCGCGCCATGCAATGTATCTTGTACAGTTCAAAAGTTAGGACAACAAGCGTATATGA
TTACACAATTAGGTAATGATGCATTTGGAGATAGCATTATTGAAACGATATCAAGTATTGGTGTAGATGTATCAAAAGTG
TATCGCACGAATGAAGCAAACACTGCGTTAGCTTTTGTGAGTTTGACTGAAGCGGGCGAGCGAGATTTTTCTTTTTATCG
TAAGCCCTCTGCTGATATGTTATTTGAACCAAGTTTTGTGAATGATATTGATGTGAATGAAAATGATGTAGTACATTTTT
GTTCTGTTGATTTGGTCGATAGTCCGATGAGAGATGCACATTATCAATTGATTACCAAAACATTAAATGCAAATGGTACT
GTCGTATTTGATCCTAATGTAAGATTGCCATTATGGGATAACGCTGAAGATTTAAGACAAACTATTCATACCTTTTTGCC
ATTGGCGCATATTGTAAAAGTTTCAGATGAAGAATTAGAATTTATTACAGGTATTCATGATGAAAATGAAGCCATTCAAT
CATTATTTACAGGTAATGTTACTGTAGTCATTTATACGAAGGGAGCAGATGGTGCTGCCGTATATTTGAAAAATGGTATT
AATCATTATCATAGTGGCTATAAGGTAAAACCTGTTGATACAACTGGGGCAGGGGATGCTTTTATTGGTGCAGTTATTAG
CAGGATTTTAGCAACCGATGTGTTAAATTTAACTCAGTTATTTGAAAATGAAGGTGAGGAAATTTTAGCGTTTAGCAATC
GCGTTGCTGCAATAGTGACAACGAAGTATGGTGCCATTAATAGTTTGCCAACATTACAAGAGGTTGAACAAGCATTTTAA

Upstream 100 bases:

>100_bases
GAATTTTCCCGACAGAAGACGCACTAGGTATTAAAACATCAACAGAATCAGGGCAAGTATATTTACAATTTACTAAATAT
GATTTAAAGGATGAACATAA

Downstream 100 bases:

>100_bases
ATAATTCATTTATAAAACAGTGTAGGAAACAAGTTGAATCTTGTGCTACACTGTTTTTTAAAACAAAATAAGATTCACGG
GGTAGGAAATTGTAGCATTT

Product: fructokinase

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVSCTVQKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKV
YRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGI
NHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF

Sequences:

>Translated_319_residues
MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVSCTVQKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKV
YRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGI
NHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF
>Mature_319_residues
MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVSCTVQKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKV
YRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGI
NHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87082343, Length=317, Percent_Identity=26.813880126183, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082281, Length=272, Percent_Identity=23.8970588235294, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081971, Length=325, Percent_Identity=22.7692307692308, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790193, Length=289, Percent_Identity=24.2214532871972, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532905, Length=293, Percent_Identity=27.9863481228669, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 35008; Mature: 35008

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVSCTVQKLGQQAYMITQLGND
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEEEEECCCC
AFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFV
CCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEECCCCC
NDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGTVVFDPNVRLPLWDNAEDLRQ
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHHHHH
TIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGI
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
NHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVT
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
TKYGAINSLPTLQEVEQAF
ECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVSCTVQKLGQQAYMITQLGND
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEEEEECCCC
AFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFV
CCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCEEECCCCC
NDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGTVVFDPNVRLPLWDNAEDLRQ
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHHHHH
TIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGI
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
NHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVT
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
TKYGAINSLPTLQEVEQAF
ECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]