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The map label for this gene is gusB [H]

Identifier: 15899743

GI number: 15899743

Start: 2785982

End: 2787694

Strand: Reverse

Name: gusB [H]

Synonym: SSO3036

Alternate gene names: 15899743

Gene position: 2787694-2785982 (Counterclockwise)

Preceding gene: 15899746

Following gene: 15899741

Centisome position: 93.16

GC content: 37.71

Gene sequence:

>1713_bases
ATGAGATCGTTTTATAGACCTAAAATAGACCTTCAAGGCTTCTGGAAATTCAAGATAGATAACGAGAATACTGGGGAAGA
GAATGGGTGGTATAAAGGCTTAGAATCTGAAGATATTATTTACGTACCAGCTTCTTGGAATGAGCAAAACCCAAAGTGGG
ATCAGTTTTCTGGGATAGCGTGGTACCAAAAGGACTTGTTCGTTAGCAACGATAATGGAAATAGGAAGGCTTGGATGGTA
TTTGAGGGGGCTGGATACATCACAAAACTTTGGATTAACGGAGAATATGGGGGGACTCATGAGGGATCATTTACCCAGTT
TAAATTTCCTATTAAACTTAAAGTTAATGAATTTAATAAAATAGTAGTGAAGATAGATAACACGCCATCCCCCTATAACT
TACCCCCAGCCAGAGATCTAAACAATGCAGCATTTGATTTCTTTAACTATGGGGGAATCCATAGGCCGGTTTACATAGAG
TTTGTAGATGAGTGTCACGTAGAAGACATAACGGTATATACTAAATCCTATGGGCACCTAAAGGTAGAAATTTTGTCAGA
ATGTAATCAAAGGTTCAGCTTAAGGTTTAAATTAGTTGATAAAGAGGGAAGGGTAATATTGAATGAGGAGAGTAGTAACG
AAGTATTCGAAAAGGATGTAAATAATGTTATACCTTGGTCTCCAGATAATCCCTACTTATACACTCTCATAGTGGAAATG
TACGTTGGTGGTAATCTGAAGGATTCGGTTTATGAACGTATAGGCTTTAGAGATGTAGAGGTGAAGGATGGCAAAATATA
CTTAAACGGAAAACCCATATTCTTGAAGGGCTTTGGAAGGCATGAGGATTTTCCAATACTAGGCAAATTTACCTATGGGG
CAGTATTAGTTAGGGATTTTTACCTTATGAGAAAAATTGGAGCAAACTCCTTCAGAACTTCCCACTACCCTTATTCAAAC
GAACACTTAGATTTAGCTGATGAAATGGGATTTTTGGTCATACTGGAACCACCTTTATGTTATTCCAATATCAGTAGAGT
AATGAGCCAAGAGGAGATTGCTAAGATGTTCGGCGATGTGAAGTACTTCGAAAAAGTAAGGGATACGATAAAGGAAATGA
TTAGACAACATAAAAATAGGCCTTCTGTGATAATGTATAGTGTAATGAATGAACCTCCAAGCGATATACGTGAGGTTGCA
GAATTCATAAGGAGGGAAGTAGAACTCTTTAAGAGTTTGGACTCTTCCAGACCAGTAACTTTTGCTTCTCATAGATCAGT
ACGAGACCTAGCATTGGAATACGTTGACGTAATTTCCTTAAATTATTACCATGGATGGTATACTGAGTGGGGGGATATTG
ATAGTGGTGTAAAGGTTGTCGCAATAGAGTTGGAGGAAATCCATAAGAAATTTCCAGAGAAGCCAATAATCATTACTGAA
TTTGGTGCTGATGCCATATACGGTCTTCATAGCGATCCTCCCCAAATGTGGTCAGAGGAATATCAGAGTGAAATGATAAG
AAAGTATATAGAAGCATTGAGGGAGAAAGATTATATTGTAGGCTTTCACATATGGAACTTCGCCGACTTTAGGACCCCTC
AAAATCCAAGCAGAACAATACTTAATAGGAAAGGAATATTTACTAGAGATAGACAGCCAAAGTTGGCAGCTAAAGTAGTA
GAGGAATTATTCAAAAATAAATTGCGTTCTTAA

Upstream 100 bases:

>100_bases
AGTATGAGACTACCTATTTAATCCTTTAGCGTTACTATTTACGAGTTTGTAATATATATTAACAAAGCTTTAATTAGATT
CTCTTGTCTGTAGATATTAT

Downstream 100 bases:

>100_bases
AGTGATAGCAAGAGTATATTATGGTTTTACCTTTGTAATAATGATGGCATATGTCTTAACCTGTTGGGGGCTAAGGGAAG
TCCCTTCATATTAGTGCAAT

Product: Beta-glucuronidase (gusB)

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRSFYRPKIDLQGFWKFKIDNENTGEENGWYKGLESEDIIYVPASWNEQNPKWDQFSGIAWYQKDLFVSNDNGNRKAWMV
FEGAGYITKLWINGEYGGTHEGSFTQFKFPIKLKVNEFNKIVVKIDNTPSPYNLPPARDLNNAAFDFFNYGGIHRPVYIE
FVDECHVEDITVYTKSYGHLKVEILSECNQRFSLRFKLVDKEGRVILNEESSNEVFEKDVNNVIPWSPDNPYLYTLIVEM
YVGGNLKDSVYERIGFRDVEVKDGKIYLNGKPIFLKGFGRHEDFPILGKFTYGAVLVRDFYLMRKIGANSFRTSHYPYSN
EHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFGDVKYFEKVRDTIKEMIRQHKNRPSVIMYSVMNEPPSDIREVA
EFIRREVELFKSLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKVVAIELEEIHKKFPEKPIIITE
FGADAIYGLHSDPPQMWSEEYQSEMIRKYIEALREKDYIVGFHIWNFADFRTPQNPSRTILNRKGIFTRDRQPKLAAKVV
EELFKNKLRS

Sequences:

>Translated_570_residues
MRSFYRPKIDLQGFWKFKIDNENTGEENGWYKGLESEDIIYVPASWNEQNPKWDQFSGIAWYQKDLFVSNDNGNRKAWMV
FEGAGYITKLWINGEYGGTHEGSFTQFKFPIKLKVNEFNKIVVKIDNTPSPYNLPPARDLNNAAFDFFNYGGIHRPVYIE
FVDECHVEDITVYTKSYGHLKVEILSECNQRFSLRFKLVDKEGRVILNEESSNEVFEKDVNNVIPWSPDNPYLYTLIVEM
YVGGNLKDSVYERIGFRDVEVKDGKIYLNGKPIFLKGFGRHEDFPILGKFTYGAVLVRDFYLMRKIGANSFRTSHYPYSN
EHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFGDVKYFEKVRDTIKEMIRQHKNRPSVIMYSVMNEPPSDIREVA
EFIRREVELFKSLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKVVAIELEEIHKKFPEKPIIITE
FGADAIYGLHSDPPQMWSEEYQSEMIRKYIEALREKDYIVGFHIWNFADFRTPQNPSRTILNRKGIFTRDRQPKLAAKVV
EELFKNKLRS
>Mature_570_residues
MRSFYRPKIDLQGFWKFKIDNENTGEENGWYKGLESEDIIYVPASWNEQNPKWDQFSGIAWYQKDLFVSNDNGNRKAWMV
FEGAGYITKLWINGEYGGTHEGSFTQFKFPIKLKVNEFNKIVVKIDNTPSPYNLPPARDLNNAAFDFFNYGGIHRPVYIE
FVDECHVEDITVYTKSYGHLKVEILSECNQRFSLRFKLVDKEGRVILNEESSNEVFEKDVNNVIPWSPDNPYLYTLIVEM
YVGGNLKDSVYERIGFRDVEVKDGKIYLNGKPIFLKGFGRHEDFPILGKFTYGAVLVRDFYLMRKIGANSFRTSHYPYSN
EHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFGDVKYFEKVRDTIKEMIRQHKNRPSVIMYSVMNEPPSDIREVA
EFIRREVELFKSLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKVVAIELEEIHKKFPEKPIIITE
FGADAIYGLHSDPPQMWSEEYQSEMIRKYIEALREKDYIVGFHIWNFADFRTPQNPSRTILNRKGIFTRDRQPKLAAKVV
EELFKNKLRS

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=601, Percent_Identity=34.6089850249584, Blast_Score=315, Evalue=1e-85,
Organism=Escherichia coli, GI1787903, Length=581, Percent_Identity=33.9070567986231, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI48994920, Length=488, Percent_Identity=25.2049180327869, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786539, Length=366, Percent_Identity=24.5901639344262, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17510825, Length=604, Percent_Identity=30.2980132450331, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19922582, Length=598, Percent_Identity=32.6086956521739, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI62471735, Length=598, Percent_Identity=32.6086956521739, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24655438, Length=598, Percent_Identity=32.6086956521739, Blast_Score=297, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24651745, Length=565, Percent_Identity=32.3893805309734, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 66797; Mature: 66797

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFYRPKIDLQGFWKFKIDNENTGEENGWYKGLESEDIIYVPASWNEQNPKWDQFSGIA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEE
WYQKDLFVSNDNGNRKAWMVFEGAGYITKLWINGEYGGTHEGSFTQFKFPIKLKVNEFNK
EEEEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEEEEEEECCCEE
IVVKIDNTPSPYNLPPARDLNNAAFDFFNYGGIHRPVYIEFVDECHVEDITVYTKSYGHL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCE
KVEILSECNQRFSLRFKLVDKEGRVILNEESSNEVFEKDVNNVIPWSPDNPYLYTLIVEM
EEHHHHHCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEH
YVGGNLKDSVYERIGFRDVEVKDGKIYLNGKPIFLKGFGRHEDFPILGKFTYGAVLVRDF
HHCCCCHHHHHHHCCCEEEEEECCEEEECCCEEEEEECCCCCCCCEEECHHHHHHHHHHH
YLMRKIGANSFRTSHYPYSNEHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFGDV
HHHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KYFEKVRDTIKEMIRQHKNRPSVIMYSVMNEPPSDIREVAEFIRREVELFKSLDSSRPVT
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKVVAIELEEIHKKFPEKPIIITE
EECCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEE
FGADAIYGLHSDPPQMWSEEYQSEMIRKYIEALREKDYIVGFHIWNFADFRTPQNPSRTI
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCHHH
LNRKGIFTRDRQPKLAAKVVEELFKNKLRS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSFYRPKIDLQGFWKFKIDNENTGEENGWYKGLESEDIIYVPASWNEQNPKWDQFSGIA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEE
WYQKDLFVSNDNGNRKAWMVFEGAGYITKLWINGEYGGTHEGSFTQFKFPIKLKVNEFNK
EEEEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEEEEEEECCCEE
IVVKIDNTPSPYNLPPARDLNNAAFDFFNYGGIHRPVYIEFVDECHVEDITVYTKSYGHL
EEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCE
KVEILSECNQRFSLRFKLVDKEGRVILNEESSNEVFEKDVNNVIPWSPDNPYLYTLIVEM
EEHHHHHCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEH
YVGGNLKDSVYERIGFRDVEVKDGKIYLNGKPIFLKGFGRHEDFPILGKFTYGAVLVRDF
HHCCCCHHHHHHHCCCEEEEEECCEEEECCCEEEEEECCCCCCCCEEECHHHHHHHHHHH
YLMRKIGANSFRTSHYPYSNEHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFGDV
HHHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KYFEKVRDTIKEMIRQHKNRPSVIMYSVMNEPPSDIREVAEFIRREVELFKSLDSSRPVT
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKVVAIELEEIHKKFPEKPIIITE
EECCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEE
FGADAIYGLHSDPPQMWSEEYQSEMIRKYIEALREKDYIVGFHIWNFADFRTPQNPSRTI
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCHHH
LNRKGIFTRDRQPKLAAKVVEELFKNKLRS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]