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The map label for this gene is hpcE-3 [H]

Identifier: 15899650

GI number: 15899650

Start: 2686213

End: 2687079

Strand: Reverse

Name: hpcE-3 [H]

Synonym: SSO2941

Alternate gene names: 15899650

Gene position: 2687079-2686213 (Counterclockwise)

Preceding gene: 15899655

Following gene: 15899648

Centisome position: 89.8

GC content: 36.91

Gene sequence:

>867_bases
ATGGTTAGATATGTTTCATTTTACGTTGGTAACCTTAGGAAGATAGGCGTAGTTGAAGGAAACTATGTATATGAGATTTC
TGAAATTGGTAAGGAGGAGGAAAGAGGAAATTCCTATAGATTAGACGAAATAAATGTGGATATTCCGATACAGCCGTCAG
CAATAATATGCACATTAGTGAATTCGCCTAAAATGGTCGGGGTTAATGACAAAAATGAAGCTAAGGAAATGGTGAAAAGT
CCGAAATTTTTCATAAAACTCCCCACTGTGGCAATAGCTCATAGACAGCCTATTATATCTCCCCCGGATGCCATAAGACC
CGAGGTGGAAATTGGAGTTATAACCAGATTCAAAATGAAAGACGTGAGCAGAAGCGATGTTAAGAAATATATCCTAGGAT
ATACGGTATTTAATGATATAACTTATCCTCCCGGTCTAAAGGAAGACTCGTATTACGCTATGCGAAGAGATCCTACAGAT
GGTAATGTTAAGAAGCTATTAATGAGGGGTACTCATTTTAGGAATAAGGTTAGGGATACCTTTGCTCCAATAGGACCTTA
TATTGTCACCGAGGAAGAGATAGGCGATGTTAACTCCTTAAGGATGAGAAGTTATTACAATGGTGAATTAGTACAAGATG
GGTATTCAAGTGAATTTATATTCTCTATAGAGGAAATACTATCAGAGCTATCAAAAATAGTTACAATTCCCCCGTTTAGT
GTAATATCTACTGGAAGCATTGGTTATACTAACGTTTCTGACGCTTCTGAGTTTAACCTAAAGCCAATTGAAAGCGCACT
AATGGTAGCTGAAGTCGAGAAGATAGGTAGATTAGAAAATCCAATAAAGATTTACAAATCAAGTTAG

Upstream 100 bases:

>100_bases
GAAAATTATGGTTTACTGCTTAATAGTTTTTCCTAAAACTAGTGCGATAAAACTAGTTTGATTAAACACATATCTACCTC
TACTAGAATTAGACTATACT

Downstream 100 bases:

>100_bases
TGTGTTTATATTTTAAAGAATATTCAAGTTTCCTTTACAAATGGTAATATCTCATCTGATTAAGGGTAATTAAATGAAGA
AATTATTGTGTACATATGTT

Product: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLVNSPKMVGVNDKNEAKEMVKS
PKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMKDVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTD
GNVKKLLMRGTHFRNKVRDTFAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFS
VISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS

Sequences:

>Translated_288_residues
MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLVNSPKMVGVNDKNEAKEMVKS
PKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMKDVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTD
GNVKKLLMRGTHFRNKVRDTFAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFS
VISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS
>Mature_288_residues
MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLVNSPKMVGVNDKNEAKEMVKS
PKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMKDVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTD
GNVKKLLMRGTHFRNKVRDTFAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFS
VISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=202, Percent_Identity=28.2178217821782, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40786394, Length=201, Percent_Identity=27.363184079602, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787428, Length=208, Percent_Identity=27.8846153846154, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572127, Length=264, Percent_Identity=25.3787878787879, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 32559; Mature: 32559

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLV
CEEEEEEEECCCEEEEEECCCEEEEHHHCCCHHHCCCCEEEEEEEEECCCCCCEEEEEEE
NSPKMVGVNDKNEAKEMVKSPKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMK
CCCEEEECCCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCEEEEEEEEEECC
DVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTDGNVKKLLMRGTHFRNKVRDT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHH
FAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFS
HCCCCCEEEEHHHHCCCHHEEEHHCCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCHH
VISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS
HEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLV
CEEEEEEEECCCEEEEEECCCEEEEHHHCCCHHHCCCCEEEEEEEEECCCCCCEEEEEEE
NSPKMVGVNDKNEAKEMVKSPKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMK
CCCEEEECCCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCEEEEEEEEEECC
DVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTDGNVKKLLMRGTHFRNKVRDT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHH
FAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFS
HCCCCCEEEEHHHHCCCHHEEEHHCCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCHH
VISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS
HEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA