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The map label for this gene is maeB [C]

Identifier: 15899581

GI number: 15899581

Start: 2623145

End: 2624443

Strand: Direct

Name: maeB [C]

Synonym: SSO2869

Alternate gene names: 15899581

Gene position: 2623145-2624443 (Clockwise)

Preceding gene: 15899576

Following gene: 15899582

Centisome position: 87.66

GC content: 40.49

Gene sequence:

>1299_bases
ATGATATATTTTTTCATGGTTGATGCTGTAGAACTGTCAAGGAAATACGAGGGAAAAATTGAGATATATCCGAAAGTTCC
TATCACCTCTTATAACGATTTCGCTCTCATATACACCCCAGGTGTTGCTGAGGTATCTAAGGCGATTTATAAGGATCCGG
ATAAGAGCTTTGAGTTAACAAGTAGGTGGAACACGATAGCAGTAGTAACAGACGGAACTAGGGTTTTGGGATTAGGAAAT
ATTGGCCCAACTGCAGCAATGCCAGTGATGGAAGGGAAATCTCTATTGTTCAAATACTTAGGAGGAGTTGACGCAATACC
TTTGCCAATTAACGTAAAGAGTGCAGATGAGTTCACAAATGTTGTAAAAGCTCTGGAAACGTCCTTTGGCGGGATTAATC
TTGAAGATATTGAGTCACCAAAGTGTTTCTATATTCTGGAAAAATTGCAATCCATGTTGAATATTCCAGTATGGCATGAT
GATCAACAAGGTACTGCGGCAGCTATCCTAGCAGGGTTGATTAATGCGATGATATTAACCAATAAAGACCCTAAGGAAAG
TAAAGTCGTATTTTACGGAGCTGGAGCATCAAATATTGCAGCAGCTAGAATTTTAGCTAAATATGGATTCAAATACGAAA
ATATTGTAATGATTGATAGCAAAGGTCCATTGTATGCTGATAGAGAGGACGTAGATCACTTAATGTTACATCATAAGTGG
AAGTACGAATTGGCTATAAAGACGAATAAATGGGAAGCTAAGGAGATAAAGGACGCGTTTAGTGGCGCAGATGCAGTAGT
TGCGGCATCTACTCCAGGTCCCAATGTAATAAAGAAGGAATGGATAAGTCTGATGAATAAGGAGGCAATAGTCTTTGCCT
TAGCCAATCCAGTTCCAGAGATATGGCCGCAAGACGCTAAGGAGGCAGGAGCGAAGATTGTTGCAACGGGTAGAAGTGAT
TTCCCTAACCAAGTTAATAACTCCCTAATTTTCCCGGCAATATTTAGGGGAGTTTTGGATAGTAGAGCAAAGGCGATAAC
AGATGAGATGACGATTGCAGCCTCTGAGGCTCTAGCTAAATACGCTAGGGATAGGGGGATAAATGAGAACTACATAATAC
CTAGAATGGATGAATGGGAGGCGTTTTATGAGGAAGCAGCAGCAGTTGCTAGTAAGGCAGTAGAATTAGGGTTAGCTAGA
GTTAAAAAGAGTAGAGAAGAGTTCAGGGAAATCGCTAAGGAGAGAATAATAAGGGCTAGGAAGATAATGAATTTGGTGAT
ATCCACATGGTCACCATAA

Upstream 100 bases:

>100_bases
AAGGTAGCAGGTATTTTAATATTTTCACGTGCTAAATGTTTGGGAGTAAACTTTAAAGTTACCTAACAAAAGTCAGCGTA
TTCATTTATAAATTTGTTTT

Downstream 100 bases:

>100_bases
GGAGGGCTACTAAAGAGGACGTCAAAACGTTAATAGATTTTTTCAGTAGGATGTATAGGTTAAACAGCGAATTCGATCCA
CTACTATTGACTCCAGAGAA

Product: NAD-dependent malic enzyme (malate oxidoreductase)

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLGLGN
IGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHD
DQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKW
KYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAKIVATGRSD
FPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLAR
VKKSREEFREIAKERIIRARKIMNLVISTWSP

Sequences:

>Translated_432_residues
MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLGLGN
IGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHD
DQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKW
KYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAKIVATGRSD
FPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLAR
VKKSREEFREIAKERIIRARKIMNLVISTWSP
>Mature_432_residues
MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLGLGN
IGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHD
DQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKW
KYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAKIVATGRSD
FPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLAR
VKKSREEFREIAKERIIRARKIMNLVISTWSP

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=366, Percent_Identity=24.8633879781421, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4505145, Length=375, Percent_Identity=25.8666666666667, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788806, Length=397, Percent_Identity=43.0730478589421, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87081919, Length=408, Percent_Identity=27.4509803921569, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17537199, Length=418, Percent_Identity=26.555023923445, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707242, Length=400, Percent_Identity=25.75, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI78707238, Length=400, Percent_Identity=25.75, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24646388, Length=448, Percent_Identity=25.4464285714286, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24646386, Length=448, Percent_Identity=25.4464285714286, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI78707240, Length=387, Percent_Identity=25.8397932816537, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 47941; Mature: 47941

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELT
CEEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEE
SRWNTIAVVTDGTRVLGLGNIGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTN
CCCCEEEEEECCCEEEEECCCCCCCCCCHHCCHHHHHHHHCCCCCCCCEEECCCHHHHHH
VVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHDDQQGTAAAILAGLINAMILT
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHEEC
NKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKW
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCE
KYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPE
EEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
IWPQDAKEAGAKIVATGRSDFPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAK
CCCCCHHHCCCEEEEECCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLARVKKSREEFREIAKERIIRAR
HHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIMNLVISTWSP
HHHHHHHHCCCC
>Mature Secondary Structure
MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELT
CEEEEEEHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEE
SRWNTIAVVTDGTRVLGLGNIGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTN
CCCCEEEEEECCCEEEEECCCCCCCCCCHHCCHHHHHHHHCCCCCCCCEEECCCHHHHHH
VVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHDDQQGTAAAILAGLINAMILT
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHEEC
NKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKW
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCE
KYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPE
EEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCC
IWPQDAKEAGAKIVATGRSDFPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAK
CCCCCHHHCCCEEEEECCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLARVKKSREEFREIAKERIIRAR
HHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIMNLVISTWSP
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]