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The map label for this gene is ilvB-4 [H]

Identifier: 15899488

GI number: 15899488

Start: 2530455

End: 2532050

Strand: Reverse

Name: ilvB-4 [H]

Synonym: SSO2774

Alternate gene names: 15899488

Gene position: 2532050-2530455 (Counterclockwise)

Preceding gene: 15899489

Following gene: 15899487

Centisome position: 84.62

GC content: 37.72

Gene sequence:

>1596_bases
ATGGGCAAAACTACGTCCGAGCTTCTAATTGATACGATTTCTTCTCAAGTTACCGACGTATTCGGGATACCTGGAACCCA
TGGTTTATCATTATACGAGGAGCTCAGAAAAAGGGTAAGTAGAGGGGAAATTAGATACTATATGCCTAGATTAGAATACG
GAGGGGCAATAATGGCAGACTATTATGCTAGATTAAAGGGAAATGTGGGAGTTTTCTTATCAGTAAATGGTCCTGGCTTT
ACTAATTCTTTAACTGCTCTGGTCGGCGCTTATTCTGAAGGTTCTCCTCTTGTCCTTATCTCCCTCAATAAGGAATTTAA
ATATAGACATAGGAGACAACTTCACGATTCTGGCTATTACGACTTACAGTTAGAAATGGCCAGACAAGCAACTAAGGCAT
CATTTAGAATTTACTCTCCAGAAGATGTGCCAATTATAATGGAAAGGGCTTTTAAAATAGCTCTCGAAGATAAGATGGGA
CCGGTTTACATTGAGGTTCCGGTCGATGTATTGGAAGAGAAAGGTGATTTTGAGAATTATAAGATTAAGAAGGTTAATAG
AACATTAATTTATCCTACGAAAGAGGAAGTAAGGGAAGCGTTAAATTTCTTGAGTGAGTGTTCTAAACCAATTCTACTAT
TAGGTTATGGTGCGTCTAGATCGAACATCTTGAACTACATTGAAAAATTAGGGATTCCCGTATTCACCACAATAAGGGGT
AAGGGAAGCATTCCGGAGAATCATCCCTTATATGCTGGAACAATATTTAACCTCAAGGAGATACCAGGGGATTGCCTCAT
AGCACTAGGGACATCATTTAACGATCTCGAAACTAGTAGATGGAGCATTAAATTGCCGGATAGGATACTTCACGTGGATC
CGGACGTTAACGTATTTAACACCTCAATAAATGCAGAAGTTACTATAAAAGCAAGTGCCGAAGCTTTTCTAGAGGAGATC
GTTGAGAAGGTTAATTTGCCTAAATGGAGTTATAAAGTGGAGGAAAAGAACAGCGATATAGTTGATAACACAAGTGAAAT
AACTCATGATTACTTAGCTAAAGTTTTAGATGAGACGTTAAGTGAAGATAGGGTTATCATCTCTGATGCAGGGACAAATC
AAGTTATGGCAATGGATATAAAAGTGTATAAACCGAACTCATACTTTAATTCGCTTATCTTTAACGCAATGGGATCTGCT
ATTCCAGCTAGCATAGGGGGTAAAATTGCATCTCCAGAGAGGCAAATAGTGAGTATTATAGGAGATCTAGGATTTCAAGG
ATGTTTTAATGAACTAATTACTGCAGCACAGTATAAGATCAACTTCTTAACAGTTTTAGTAGAGGATGGTGTACAGCACT
TCCTAAGGTTAAATCAGAAAATGAGATATGGAAATACTTTTACAACTGATGTATTTCAAATAGATTACACTAAGGTTGTG
GAAGGGATTGGGGTTAACGTAATTGAGGTTAAGGATAGGAAAGACCTTAAGAAAAGTGTAGAAGAGGCCGTTGGATTATC
TCTCAAGAGTCCAACAGTTCTAAGAGTTCACGTTAGCCCTAATAGTATACCTTCTAGATTGTTAATGAAAAGATAG

Upstream 100 bases:

>100_bases
GAGTCTTTAAATTATGATTGGGACAAGAGTTCTGAGAAATATTATAATTACTTGATTCGAAAAGCATTAGATTAATTTAT
AGGGATTATAAAAATAATTT

Downstream 100 bases:

>100_bases
TTATTTATATTAAAAATAAGGAGTTTTACCTATGGTTAAAATCCTATTATTCTCTCCAAATCCTAACGAATTTAAGAGAG
TTGGTGTTTATGAGAACGGT

Product: acetolactate synthase large subunit IlvB

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEIRYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGF
TNSLTALVGAYSEGSPLVLISLNKEFKYRHRRQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIIMERAFKIALEDKMG
PVYIEVPVDVLEEKGDFENYKIKKVNRTLIYPTKEEVREALNFLSECSKPILLLGYGASRSNILNYIEKLGIPVFTTIRG
KGSIPENHPLYAGTIFNLKEIPGDCLIALGTSFNDLETSRWSIKLPDRILHVDPDVNVFNTSINAEVTIKASAEAFLEEI
VEKVNLPKWSYKVEEKNSDIVDNTSEITHDYLAKVLDETLSEDRVIISDAGTNQVMAMDIKVYKPNSYFNSLIFNAMGSA
IPASIGGKIASPERQIVSIIGDLGFQGCFNELITAAQYKINFLTVLVEDGVQHFLRLNQKMRYGNTFTTDVFQIDYTKVV
EGIGVNVIEVKDRKDLKKSVEEAVGLSLKSPTVLRVHVSPNSIPSRLLMKR

Sequences:

>Translated_531_residues
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEIRYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGF
TNSLTALVGAYSEGSPLVLISLNKEFKYRHRRQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIIMERAFKIALEDKMG
PVYIEVPVDVLEEKGDFENYKIKKVNRTLIYPTKEEVREALNFLSECSKPILLLGYGASRSNILNYIEKLGIPVFTTIRG
KGSIPENHPLYAGTIFNLKEIPGDCLIALGTSFNDLETSRWSIKLPDRILHVDPDVNVFNTSINAEVTIKASAEAFLEEI
VEKVNLPKWSYKVEEKNSDIVDNTSEITHDYLAKVLDETLSEDRVIISDAGTNQVMAMDIKVYKPNSYFNSLIFNAMGSA
IPASIGGKIASPERQIVSIIGDLGFQGCFNELITAAQYKINFLTVLVEDGVQHFLRLNQKMRYGNTFTTDVFQIDYTKVV
EGIGVNVIEVKDRKDLKKSVEEAVGLSLKSPTVLRVHVSPNSIPSRLLMKR
>Mature_530_residues
GKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEIRYYMPRLEYGGAIMADYYARLKGNVGVFLSVNGPGFT
NSLTALVGAYSEGSPLVLISLNKEFKYRHRRQLHDSGYYDLQLEMARQATKASFRIYSPEDVPIIMERAFKIALEDKMGP
VYIEVPVDVLEEKGDFENYKIKKVNRTLIYPTKEEVREALNFLSECSKPILLLGYGASRSNILNYIEKLGIPVFTTIRGK
GSIPENHPLYAGTIFNLKEIPGDCLIALGTSFNDLETSRWSIKLPDRILHVDPDVNVFNTSINAEVTIKASAEAFLEEIV
EKVNLPKWSYKVEEKNSDIVDNTSEITHDYLAKVLDETLSEDRVIISDAGTNQVMAMDIKVYKPNSYFNSLIFNAMGSAI
PASIGGKIASPERQIVSIIGDLGFQGCFNELITAAQYKINFLTVLVEDGVQHFLRLNQKMRYGNTFTTDVFQIDYTKVVE
GIGVNVIEVKDRKDLKKSVEEAVGLSLKSPTVLRVHVSPNSIPSRLLMKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=529, Percent_Identity=23.6294896030246, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI87081685, Length=539, Percent_Identity=27.0871985157699, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=24.3093922651934, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=23.9776951672862, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=25.1838235294118, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=22.3591549295775, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=528, Percent_Identity=22.9166666666667, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=528, Percent_Identity=22.7272727272727, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=24.2160278745645, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321524, Length=464, Percent_Identity=23.4913793103448, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=470, Percent_Identity=21.7021276595745, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=467, Percent_Identity=21.8415417558887, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320816, Length=541, Percent_Identity=21.81146025878, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=23.304347826087, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59517; Mature: 59386

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEIRYYMPRLEYGGAIMAD
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFLSVNGPGFTNSLTALVGAYSEGSPLVLISLNKEFKYRHRRQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCE
DLQLEMARQATKASFRIYSPEDVPIIMERAFKIALEDKMGPVYIEVPVDVLEEKGDFENY
EEEEHHHHHHHHCEEEEECCCCCHHHHHHHHHEEEECCCCCEEEEECHHHHHCCCCCCCE
KIKKVNRTLIYPTKEEVREALNFLSECSKPILLLGYGASRSNILNYIEKLGIPVFTTIRG
EEEEECEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECC
KGSIPENHPLYAGTIFNLKEIPGDCLIALGTSFNDLETSRWSIKLPDRILHVDPDVNVFN
CCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEE
TSINAEVTIKASAEAFLEEIVEKVNLPKWSYKVEEKNSDIVDNTSEITHDYLAKVLDETL
ECCCEEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
SEDRVIISDAGTNQVMAMDIKVYKPNSYFNSLIFNAMGSAIPASIGGKIASPERQIVSII
CCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHH
GDLGFQGCFNELITAAQYKINFLTVLVEDGVQHFLRLNQKMRYGNTFTTDVFQIDYTKVV
HHCCHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEECEEEEEEHHHHH
EGIGVNVIEVKDRKDLKKSVEEAVGLSLKSPTVLRVHVSPNSIPSRLLMKR
HCCCCEEEEECCHHHHHHHHHHHHCCEECCCEEEEEEECCCCCHHHHHCCC
>Mature Secondary Structure 
GKTTSELLIDTISSQVTDVFGIPGTHGLSLYEELRKRVSRGEIRYYMPRLEYGGAIMAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
YYARLKGNVGVFLSVNGPGFTNSLTALVGAYSEGSPLVLISLNKEFKYRHRRQLHDSGYY
HHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCE
DLQLEMARQATKASFRIYSPEDVPIIMERAFKIALEDKMGPVYIEVPVDVLEEKGDFENY
EEEEHHHHHHHHCEEEEECCCCCHHHHHHHHHEEEECCCCCEEEEECHHHHHCCCCCCCE
KIKKVNRTLIYPTKEEVREALNFLSECSKPILLLGYGASRSNILNYIEKLGIPVFTTIRG
EEEEECEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECC
KGSIPENHPLYAGTIFNLKEIPGDCLIALGTSFNDLETSRWSIKLPDRILHVDPDVNVFN
CCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEE
TSINAEVTIKASAEAFLEEIVEKVNLPKWSYKVEEKNSDIVDNTSEITHDYLAKVLDETL
ECCCEEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
SEDRVIISDAGTNQVMAMDIKVYKPNSYFNSLIFNAMGSAIPASIGGKIASPERQIVSII
CCCEEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHH
GDLGFQGCFNELITAAQYKINFLTVLVEDGVQHFLRLNQKMRYGNTFTTDVFQIDYTKVV
HHCCHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEECEEEEEEHHHHH
EGIGVNVIEVKDRKDLKKSVEEAVGLSLKSPTVLRVHVSPNSIPSRLLMKR
HCCCCEEEEECCHHHHHHHHHHHHCCEECCCEEEEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512571 [H]