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The map label for this gene is sqdB [H]

Identifier: 15899314

GI number: 15899314

Start: 2353036

End: 2354019

Strand: Reverse

Name: sqdB [H]

Synonym: SSO2585

Alternate gene names: 15899314

Gene position: 2354019-2353036 (Counterclockwise)

Preceding gene: 15899320

Following gene: 15899313

Centisome position: 78.67

GC content: 37.8

Gene sequence:

>984_bases
ATGAACATTGAAAAAAGCTTAATATACTTTAGAATGCATATTTATACTCGTGATAATATGGTAAAAATAGCTTTTATAGG
AGTAGGAAAAATAGGCCAGACAATAGCTTATTCTGTAATTTTTGACGGCTTGGCTAGTGAAGCTATACTATATGATATTG
TACCGGAATTACCAGAAAAGTTTGAACACGAGTTAAGACACGCAATAGCTACGCGAGGTCTTTCCACTGAAGTTATTGGT
ACTAACTCTCTTGATGATGTGACCAATGCTGATATCATACTAATTATGGCAGGTAAGCCCAGGAAACCTGGAATGAGCAG
AAGGGACTTGTTTATTGACAATGCGAAAATACAAATAGACCTTGCAAAACAGCTTCCACCTAAAAATCCAGGGGCACTAT
ATATAATGGTTGCAAATCCCGTGGACATGATGGCATCAGTATTTATGAGATTCTCTAAACAATTTACAATAAGTACTGGC
GATGCAGTGGAGACAATGAGACTTAGATCATACATAGCCAAGAAACTCAAGGTCCCAGCGAACAAAGTAACTGGATACGT
AGCAGGAGAGCATGGAGAAGATGCAGTGGTATTATGGAGCACAGTTAAGGTTAATGGAAAACCTTTTGAGGAAGTAGCTA
AGGGATTAACTAAAGAGGAAGTTGAAAATTACGTCAAGTCAATACCAGGGGAAATAATAAGGGTAATGGGAGGAACAACA
TGGGGACCTGCAACTATAATAAAGGATATCGTGAGATCAGTAGTCTTTAATGAGGGAAGAGTAATGAGTATCGCAACCCC
TAGAACTTATCAAGGTGAAATAATCCACATAAGTGTTCCAATAGTTGTAGGAGCTGAAATAGGACCGAGTTTGGAGGGTG
TATTACCAGAGAGTGATAGGCAAAGGTTAAATAAGTCTGTTGAGGACTTCTATAGAGTTTATAAGGAGAATTTAGATCAT
CTATTACAGACTGTTAAGCAATAA

Upstream 100 bases:

>100_bases
ACTTAAATTTTGATAATTTACTGAACTCACAAATTCAGCAATCTTTTCAGCTATTTCCATAATAGAGTAAAATTAAAGGG
AGTATAAAAGTCGAACTATA

Downstream 100 bases:

>100_bases
TTTCCGATTTAACGGATGCGGATTTAACTTTTCTCGTTAGTTAGCCTTACCCTTACCATAAGCTTTTTTATTTCCACAAC
CTTCACATTATTGATGAAAG

Product: L-lactate dehydrogenase (sqdB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNIEKSLIYFRMHIYTRDNMVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEKFEHELRHAIATRGLSTEVIG
TNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQIDLAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTG
DAVETMRLRSYIAKKLKVPANKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTT
WGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDRQRLNKSVEDFYRVYKENLDH
LLQTVKQ

Sequences:

>Translated_327_residues
MNIEKSLIYFRMHIYTRDNMVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEKFEHELRHAIATRGLSTEVIG
TNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQIDLAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTG
DAVETMRLRSYIAKKLKVPANKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTT
WGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDRQRLNKSVEDFYRVYKENLDH
LLQTVKQ
>Mature_327_residues
MNIEKSLIYFRMHIYTRDNMVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEKFEHELRHAIATRGLSTEVIG
TNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQIDLAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTG
DAVETMRLRSYIAKKLKVPANKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTT
WGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDRQRLNKSVEDFYRVYKENLDH
LLQTVKQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=26.2135922330097, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=26.2135922330097, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI291575128, Length=310, Percent_Identity=25.4838709677419, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI4557032, Length=310, Percent_Identity=25.4838709677419, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=25.8064516129032, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI15082234, Length=306, Percent_Identity=24.8366013071895, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=25.8064516129032, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI9257228, Length=314, Percent_Identity=25.796178343949, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4504973, Length=314, Percent_Identity=25.796178343949, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI260099727, Length=216, Percent_Identity=25, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI260099725, Length=189, Percent_Identity=27.5132275132275, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI21735621, Length=291, Percent_Identity=27.8350515463918, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI207028494, Length=204, Percent_Identity=25.9803921568627, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789632, Length=225, Percent_Identity=30.2222222222222, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=295, Percent_Identity=24.7457627118644, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17554310, Length=213, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45550422, Length=271, Percent_Identity=28.7822878228782, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136226, Length=299, Percent_Identity=24.0802675585284, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647881, Length=194, Percent_Identity=31.4432989690722, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24663599, Length=182, Percent_Identity=31.8681318681319, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 36395; Mature: 36395

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEKSLIYFRMHIYTRDNMVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEK
CCCCCEEEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEHELRHAIATRGLSTEVIGTNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQID
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEE
LAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTGDAVETMRLRSYIAKKLKVPA
EHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCH
NKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTT
HHHEEEEECCCCCCEEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCC
WGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDR
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHH
QRLNKSVEDFYRVYKENLDHLLQTVKQ
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIEKSLIYFRMHIYTRDNMVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEK
CCCCCEEEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEHELRHAIATRGLSTEVIGTNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQID
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEE
LAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTGDAVETMRLRSYIAKKLKVPA
EHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCH
NKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTT
HHHEEEEECCCCCCEEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCC
WGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDR
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHH
QRLNKSVEDFYRVYKENLDHLLQTVKQ
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]