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The map label for this gene is pckG

Identifier: 15899271

GI number: 15899271

Start: 2304367

End: 2306178

Strand: Reverse

Name: pckG

Synonym: SSO2537

Alternate gene names: 15899271

Gene position: 2306178-2304367 (Counterclockwise)

Preceding gene: 15899272

Following gene: 15899268

Centisome position: 77.07

GC content: 35.82

Gene sequence:

>1812_bases
ATGAAATCTAGTTTAGATTTCTTAAACAATTTCATTCAAAGAGATGCAGTTAAAAAATTAGAATCCATTAATAATTATCC
TTTAATTGAATTTTTAAACAGTGTTGTTAAGTTGTGCGAACCAGATAGCGTATATCTTATAACTGGGAGTGATGAGGAGA
AGGAATATATTAGGAAGAAAGCTCTAGAAAGCAAGGAAGAGATAAGATTACAGACTAGTGGGCATACTATACATTTCGAT
CATCCTTTAGATCAAGCTAGAGCTAGGGAAGATACATTCATACTCAGTGATACTAAGATCCCTTATGTTAATACTAAACC
TAGAAATGAGGGTTTAAGTGAAATGCTGACTTTGCTTAAGGGTTCAATGAGAGGGAGGGAAATGTATGTGGGATTTTATT
CTCTAGGGCCCAGAAATTCCCCTTTCCAGATTTTAGCAGTTCAAGTGACTGATTCTCCATACGTCATTCATAGTGAAAAC
ATATTATATAGAATCGCATTTGAGGATTTCAGTAACAACACTAAATTTTTAAGGTTCGTCCACTCAAAAGGAGAGCCAGA
TATAAAGAAAAGAAGGATAATGATTGACCTAGCGAACAATACGGTGTATAGCGTTAACACAACTTACGCTGGTAACAGTG
TTGGTTTGAAGAAGTTAGCTTTAAGGTTAACTATAATGAAAGCTGTAGAGGAAGGTTGGCTTTCTGAGCATATGGCTATA
GTTGGATTCAATGGAGATAAAGGTATACACTATTTTACAGCGTCATTTCCCTCTGGAAGTGGGAAGACCTCTACGTCAAT
GATCGGGAATCTAATAAGCGATGATTTAGCATTTATAAGGGAATTTGAAGGCCTTCCTAAGGCAGTAAATCCGGAAATCG
GAGTTTTCGGGATTATACAAGGCATAAACGCTAGGGATGACCCAATAATATGGGAAGTTCTCCATAAACCTGGAGAAGTG
ATATTCTCTAATGTATTAATGACTGAAGATGGTGATGTATACTGGGAGGGAAGTGAATTACCAAAACCAGAAAGGGGATA
TAACCATGAGGGAAGGTGGGATAGGGAAAGTGGTAAACCAGCCTCTCATCCGAACGCTAGATTTACTGTTCCTTTAACTT
CATTTAGTAATTTAGACAAGAATTGGGATAATCCGAATGGCGTCATCATTGATGGCATAATATTTGGTGTAAGAGATTAT
AGTACTCTTGTTCCGGTTGTTGAGGCCTTTTCATGGTCACATGGAGTTATAACCATTGGAGCTTCAATGGAGTCCGCTAG
AACTTCAGCAGTAATTGGGAAGTCTGATGAGCTAGAATTTAATCCAATGGCAATTTTAGACTTCATGCCAATTTCACTTA
GTAGATATTTAAGAAATTATTTGAATTTCGGCAAGAGATTAAGGAAAAGTCCTAAAATATTTGGTTTCAACTATTTTCTA
AAAGATGATAATAAGTTCTTGAACTCAAAGGAAGATAAGAAAGTATGGGTTAGCTGGGCAGTAAAAAGAGTGGAGGAAAC
TGCTAATGCGATCTATACACCTATTGGTCTTATCCCATTTTATGAAGATCTTAAAGCCCTTTTCAAACGAGTTTTAGGAA
AGGAATACGGTAAGGAAGAGTATGAAAAGCAATTCACAATAAAATTGAGGAAATACTTAGAGAAAACCGAACGTATAATT
GAAATTTACCTTAAATTTGAAGATATACCATCTGAAGTAATCAACGAGTTGAAGATGCAAAAAGAGAGAATTGTCGATTA
CATCAATAAATACGGTGATTCAGTATCGCCATTTAGATTAGAAAAAGATTGA

Upstream 100 bases:

>100_bases
TTAAATTTATTTTCAATACAGTGATGTTTAGAGCCCATCGTAAAGTATTATTATTAGTTAAAAGGAAAACATTTATGCTC
TTAAATACTATACATATAAC

Downstream 100 bases:

>100_bases
AATATCTTATATCTTCTAACTCTATTGAGAATAAATATTTTGTAAAAAAATAACTTAAGTTTTTCCTTTTTATGGTATGA
GTACTTGTCTTCCAATAGCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MKSSLDFLNNFIQRDAVKKLESINNYPLIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKKALESKEEIRLQTSGHTIHFD
HPLDQARAREDTFILSDTKIPYVNTKPRNEGLSEMLTLLKGSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSEN
ILYRIAFEDFSNNTKFLRFVHSKGEPDIKKRRIMIDLANNTVYSVNTTYAGNSVGLKKLALRLTIMKAVEEGWLSEHMAI
VGFNGDKGIHYFTASFPSGSGKTSTSMIGNLISDDLAFIREFEGLPKAVNPEIGVFGIIQGINARDDPIIWEVLHKPGEV
IFSNVLMTEDGDVYWEGSELPKPERGYNHEGRWDRESGKPASHPNARFTVPLTSFSNLDKNWDNPNGVIIDGIIFGVRDY
STLVPVVEAFSWSHGVITIGASMESARTSAVIGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKRLRKSPKIFGFNYFL
KDDNKFLNSKEDKKVWVSWAVKRVEETANAIYTPIGLIPFYEDLKALFKRVLGKEYGKEEYEKQFTIKLRKYLEKTERII
EIYLKFEDIPSEVINELKMQKERIVDYINKYGDSVSPFRLEKD

Sequences:

>Translated_603_residues
MKSSLDFLNNFIQRDAVKKLESINNYPLIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKKALESKEEIRLQTSGHTIHFD
HPLDQARAREDTFILSDTKIPYVNTKPRNEGLSEMLTLLKGSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSEN
ILYRIAFEDFSNNTKFLRFVHSKGEPDIKKRRIMIDLANNTVYSVNTTYAGNSVGLKKLALRLTIMKAVEEGWLSEHMAI
VGFNGDKGIHYFTASFPSGSGKTSTSMIGNLISDDLAFIREFEGLPKAVNPEIGVFGIIQGINARDDPIIWEVLHKPGEV
IFSNVLMTEDGDVYWEGSELPKPERGYNHEGRWDRESGKPASHPNARFTVPLTSFSNLDKNWDNPNGVIIDGIIFGVRDY
STLVPVVEAFSWSHGVITIGASMESARTSAVIGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKRLRKSPKIFGFNYFL
KDDNKFLNSKEDKKVWVSWAVKRVEETANAIYTPIGLIPFYEDLKALFKRVLGKEYGKEEYEKQFTIKLRKYLEKTERII
EIYLKFEDIPSEVINELKMQKERIVDYINKYGDSVSPFRLEKD
>Mature_603_residues
MKSSLDFLNNFIQRDAVKKLESINNYPLIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKKALESKEEIRLQTSGHTIHFD
HPLDQARAREDTFILSDTKIPYVNTKPRNEGLSEMLTLLKGSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSEN
ILYRIAFEDFSNNTKFLRFVHSKGEPDIKKRRIMIDLANNTVYSVNTTYAGNSVGLKKLALRLTIMKAVEEGWLSEHMAI
VGFNGDKGIHYFTASFPSGSGKTSTSMIGNLISDDLAFIREFEGLPKAVNPEIGVFGIIQGINARDDPIIWEVLHKPGEV
IFSNVLMTEDGDVYWEGSELPKPERGYNHEGRWDRESGKPASHPNARFTVPLTSFSNLDKNWDNPNGVIIDGIIFGVRDY
STLVPVVEAFSWSHGVITIGASMESARTSAVIGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKRLRKSPKIFGFNYFL
KDDNKFLNSKEDKKVWVSWAVKRVEETANAIYTPIGLIPFYEDLKALFKRVLGKEYGKEEYEKQFTIKLRKYLEKTERII
EIYLKFEDIPSEVINELKMQKERIVDYINKYGDSVSPFRLEKD

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=605, Percent_Identity=32.2314049586777, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI66346721, Length=605, Percent_Identity=30.5785123966942, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI66346723, Length=355, Percent_Identity=30.4225352112676, Blast_Score=150, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71989645, Length=610, Percent_Identity=30, Blast_Score=238, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI71989658, Length=610, Percent_Identity=30, Blast_Score=238, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17555492, Length=546, Percent_Identity=31.3186813186813, Blast_Score=236, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71989653, Length=600, Percent_Identity=30.3333333333333, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI32564411, Length=557, Percent_Identity=31.2387791741472, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI32564407, Length=450, Percent_Identity=33.5555555555556, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71989649, Length=485, Percent_Identity=30.3092783505155, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI32564409, Length=295, Percent_Identity=33.8983050847458, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24655085, Length=606, Percent_Identity=31.5181518151815, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655082, Length=613, Percent_Identity=31.810766721044, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI161077226, Length=561, Percent_Identity=32.9768270944742, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24655088, Length=501, Percent_Identity=33.1337325349301, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_SULSO (Q97VS5)

Other databases:

- EMBL:   AE006641
- PIR:   C90426
- RefSeq:   NP_343876.1
- ProteinModelPortal:   Q97VS5
- GeneID:   1453993
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2537
- NMPDR:   fig|273057.1.peg.2299
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   SSOL273057:SSO2537-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 69058; Mature: 69058

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 260-260 BINDING 87-87 BINDING 211-211 BINDING 218-218 BINDING 258-258 BINDING 367-367 BINDING 398-398

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSLDFLNNFIQRDAVKKLESINNYPLIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKK
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
ALESKEEIRLQTSGHTIHFDHPLDQARAREDTFILSDTKIPYVNTKPRNEGLSEMLTLLK
HHCCHHHEEEEECCCEEEECCCHHHHHCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHH
GSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSENILYRIAFEDFSNNTKFLRFV
CCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCEEEECCCEEEEEEEECCCCCCEEEEEE
HSKGEPDIKKRRIMIDLANNTVYSVNTTYAGNSVGLKKLALRLTIMKAVEEGWLSEHMAI
ECCCCCCHHHCEEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VGFNGDKGIHYFTASFPSGSGKTSTSMIGNLISDDLAFIREFEGLPKAVNPEIGVFGIIQ
EEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GINARDDPIIWEVLHKPGEVIFSNVLMTEDGDVYWEGSELPKPERGYNHEGRWDRESGKP
CCCCCCCCCHHHHHHCCCHHEEEHEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC
ASHPNARFTVPLTSFSNLDKNWDNPNGVIIDGIIFGVRDYSTLVPVVEAFSWSHGVITIG
CCCCCCEEEEEECCHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEE
ASMESARTSAVIGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKRLRKSPKIFGFNYFL
CCCCCCHHHEEECCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
KDDNKFLNSKEDKKVWVSWAVKRVEETANAIYTPIGLIPFYEDLKALFKRVLGKEYGKEE
ECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
YEKQFTIKLRKYLEKTERIIEIYLKFEDIPSEVINELKMQKERIVDYINKYGDSVSPFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
EKD
CCC
>Mature Secondary Structure
MKSSLDFLNNFIQRDAVKKLESINNYPLIEFLNSVVKLCEPDSVYLITGSDEEKEYIRKK
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
ALESKEEIRLQTSGHTIHFDHPLDQARAREDTFILSDTKIPYVNTKPRNEGLSEMLTLLK
HHCCHHHEEEEECCCEEEECCCHHHHHCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHH
GSMRGREMYVGFYSLGPRNSPFQILAVQVTDSPYVIHSENILYRIAFEDFSNNTKFLRFV
CCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCEEEECCCEEEEEEEECCCCCCEEEEEE
HSKGEPDIKKRRIMIDLANNTVYSVNTTYAGNSVGLKKLALRLTIMKAVEEGWLSEHMAI
ECCCCCCHHHCEEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEE
VGFNGDKGIHYFTASFPSGSGKTSTSMIGNLISDDLAFIREFEGLPKAVNPEIGVFGIIQ
EEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GINARDDPIIWEVLHKPGEVIFSNVLMTEDGDVYWEGSELPKPERGYNHEGRWDRESGKP
CCCCCCCCCHHHHHHCCCHHEEEHEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCC
ASHPNARFTVPLTSFSNLDKNWDNPNGVIIDGIIFGVRDYSTLVPVVEAFSWSHGVITIG
CCCCCCEEEEEECCHHCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEE
ASMESARTSAVIGKSDELEFNPMAILDFMPISLSRYLRNYLNFGKRLRKSPKIFGFNYFL
CCCCCCHHHEEECCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
KDDNKFLNSKEDKKVWVSWAVKRVEETANAIYTPIGLIPFYEDLKALFKRVLGKEYGKEE
ECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
YEKQFTIKLRKYLEKTERIIEIYLKFEDIPSEVINELKMQKERIVDYINKYGDSVSPFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
EKD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726