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The map label for this gene is glpK-2

Identifier: 15898917

GI number: 15898917

Start: 1960696

End: 1962195

Strand: Direct

Name: glpK-2

Synonym: SSO2133

Alternate gene names: 15898917

Gene position: 1960696-1962195 (Clockwise)

Preceding gene: 15898914

Following gene: 15898922

Centisome position: 65.53

GC content: 39.07

Gene sequence:

>1500_bases
ATGCCCGGTGGCTTCATCTTAGCAATAGATGAGGGAACAACAAGTGCTAGAGCAATTATATATAATCAAGATTTAGAAGT
ATTAGGAATAGGTCAGTACGATTTTCCTCAACATTACCCAAGTCCAGGATATGTAGAGCACAATCCCGATGAGATATGGA
ATGCACAAATGTTAGCAATAAAAGAAGCTATGAAAAAGGCGAAGATAGAGTCTAGGCAAGTAGCTGGAATCGGAGTGACT
AATCAGAGGGAGACTACCATATTATGGGATGCGATAAGTGGGAAACCAATTTACAATGCAATTGTTTGGCAAGATAGAAG
AACTTCTAATATAACTGATTGGCTAAAGGAGAATTACTTTGGTATGATAAAGGATAAAACCGGGCTTATTCCTGACCCTT
ACTTTAGTGGATCTAAAATAAAGTGGATATTGGATAATCTCCCTAATGTGAGGAGTAAGGCAGAAAAGGGCGAAATAAAG
TTTGGAACTATAGATACTTATCTTATATGGAAGTTAACTAATGGTAAGATTCACGTAACTGATTATTCAAACGCGTCAAG
AACCATGCTATTCAACATAAATAAGCTAGAATGGGATAGAGAAATCTTGGAATTATTGAAAATTCCAGAGAGTATATTAC
CTGAAGTGAGACCATCTAGCGATATTTACGGGTATACTGAAGTTTTGGGATCCTCTATTCCAATCTCTGGTGATGCCGGA
GATCAACAAGCTGCCTTGTTTGGTCAAGTAGCCTATGACATGGGCGAAGTAAAATCAACCTATGGGACTGGAAGTTTCAT
TCTGATGAATATAGGTAGCAATCCGATTTTTTCAGAAAATTTACTAACCACTATAGCTTGGGGTTTAGAGAGTAAAAGGG
TTACATATGCTTTAGAAGGAAGTATATTCATAACTGGTGCGGCAGTACAGTGGTTTAGAGATGGTTTAAGAGCAATAGAT
GCATCGGATGACATCGAGCCATTAGCCGCAAGTGTACCGGATACTGGTGGTGTCTATTTCGTACCAGCATTTGTCGGGTT
AGGTGCACCATACTGGGATCCATACGCGAGGGGATTGATAATAGGTATTACGAGGGGGACTACAAAGGCTCATATAGCTA
GAGCAATACTCGAATCAATAGCATACCAAAATAGAGACGTAATAGAAATAATGGAGAAAGAGTCTGGGACTAAGATAAAC
ATACTAAAGGTTGATGGTGGTGGGGCTAAGGACAATTTACTGATGCAATTTCAAGCAGATATTTTGGGAATTAGGGTAGT
AAGGCCGAAGGTAATGGAGACTGCATCTATGGGCGTTGCAATGTTAGCAGGTTTAGCAATAAATTATTGGAATTCACTTA
ACGAATTGAAACAAAAATGGACTGTCGACAAAGAGTTCATTCCCTCAATCAATAAAGAAGAAAGGGAAAGACGCTATAAT
GCGTGGAAAGAGGCAGTGAAGAGGTCGCTGGGTTGGGAAAAATCGTTAGGTAGTAAATAA

Upstream 100 bases:

>100_bases
CGGAATGGTTAAAAGCATTCTTGACTCTACCCAGTCATTTCAGCAATAATATTGTTAGCTACATTTATTTTTTATATATT
AGTTACACAGATAGCTTATT

Downstream 100 bases:

>100_bases
CTATGGTCATTCAGTCTTTGACCAGAACCCGTTTATGACATTTTTCATATAGAGGTTCCATAGGGGAAATTTTCTGGAAT
TTCACTACTGAAACCATATA

Product: glycerol kinase (glpK-2)

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 2; Glycerokinase 2; GK 2

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAIKEAMKKAKIESRQVAGIGVT
NQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYFGMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIK
FGTIDTYLIWKLTNGKIHVTDYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAG
DQQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEGSIFITGAAVQWFRDGLRAID
ASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKIN
ILKVDGGGAKDNLLMQFQADILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYN
AWKEAVKRSLGWEKSLGSK

Sequences:

>Translated_499_residues
MPGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAIKEAMKKAKIESRQVAGIGVT
NQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYFGMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIK
FGTIDTYLIWKLTNGKIHVTDYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAG
DQQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEGSIFITGAAVQWFRDGLRAID
ASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKIN
ILKVDGGGAKDNLLMQFQADILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYN
AWKEAVKRSLGWEKSLGSK
>Mature_498_residues
PGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAIKEAMKKAKIESRQVAGIGVTN
QRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYFGMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIKF
GTIDTYLIWKLTNGKIHVTDYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAGD
QQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEGSIFITGAAVQWFRDGLRAIDA
SDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKINI
LKVDGGGAKDNLLMQFQADILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYNA
WKEAVKRSLGWEKSLGSK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI42794763, Length=511, Percent_Identity=44.8140900195695, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=45.1485148514852, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=45.1485148514852, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=512, Percent_Identity=43.5546875, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=35.2941176470588, Blast_Score=314, Evalue=1e-85,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=55.421686746988, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1790005, Length=472, Percent_Identity=24.1525423728814, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789987, Length=502, Percent_Identity=23.9043824701195, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789138, Length=514, Percent_Identity=23.3463035019455, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787789, Length=524, Percent_Identity=23.0916030534351, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789168, Length=471, Percent_Identity=23.1422505307856, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=41.7495029821074, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=25.9117082533589, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=270, Percent_Identity=32.962962962963, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=574, Percent_Identity=35.7142857142857, Blast_Score=323, Evalue=5e-89,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=42.6900584795322, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655555, Length=506, Percent_Identity=40.5138339920949, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655560, Length=506, Percent_Identity=40.5138339920949, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655570, Length=506, Percent_Identity=40.5138339920949, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655574, Length=506, Percent_Identity=40.5138339920949, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655565, Length=506, Percent_Identity=40.5138339920949, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24654573, Length=454, Percent_Identity=43.6123348017621, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=534, Percent_Identity=32.0224719101124, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=32.7902240325866, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24656367, Length=523, Percent_Identity=29.4455066921606, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI21357867, Length=534, Percent_Identity=28.2771535580524, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=31.3390313390313, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552909, Length=294, Percent_Identity=29.9319727891156, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK2_SULSO (P95907)

Other databases:

- EMBL:   Y08256
- EMBL:   AE006641
- PIR:   S74045
- RefSeq:   NP_343522.1
- ProteinModelPortal:   P95907
- SMR:   P95907
- GeneID:   1453639
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2133
- NMPDR:   fig|273057.1.peg.1945
- HOGENOM:   HBG511469
- OMA:   LGNQQAS
- PhylomeDB:   P95907
- ProtClustDB:   CLSK268256
- BioCyc:   SSOL273057:SSO2133-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55643; Mature: 55512

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 134-134 BINDING 241-241 BINDING 263-263 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAI
CCCCEEEEEECCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KEAMKKAKIESRQVAGIGVTNQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYF
HHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCC
GMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIKFGTIDTYLIWKLTNGKIHVT
EEEECCCCCCCCCCCCCCEEEEHHHCCCCHHHHCCCCCEEEEEEEEEEEEEEECCEEEEE
DYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAG
ECCCCCCEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCC
DQQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEG
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEC
SIFITGAAVQWFRDGLRAIDASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLI
CEEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCCCEE
IGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKINILKVDGGGAKDNLLMQFQAD
EEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECC
ILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYN
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHH
AWKEAVKRSLGWEKSLGSK
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAI
CCCEEEEEECCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KEAMKKAKIESRQVAGIGVTNQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYF
HHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHCCC
GMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIKFGTIDTYLIWKLTNGKIHVT
EEEECCCCCCCCCCCCCCEEEEHHHCCCCHHHHCCCCCEEEEEEEEEEEEEEECCEEEEE
DYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAG
ECCCCCCEEEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCC
DQQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEG
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEC
SIFITGAAVQWFRDGLRAIDASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLI
CEEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEHHHHCCCCCCCCCCCCEE
IGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKINILKVDGGGAKDNLLMQFQAD
EEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECC
ILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYN
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHH
AWKEAVKRSLGWEKSLGSK
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726