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The map label for this gene is gdhA-2

Identifier: 15898704

GI number: 15898704

Start: 1727019

End: 1728281

Strand: Direct

Name: gdhA-2

Synonym: SSO1907

Alternate gene names: 15898704

Gene position: 1727019-1728281 (Clockwise)

Preceding gene: 15898703

Following gene: 15898707

Centisome position: 57.72

GC content: 39.43

Gene sequence:

>1263_bases
TTGATGGAAGAAGTTCTAAGTTCGAGTCTATATACTCAACAAGTGAAAAAGTTATATAAAGTCGGAGAGTTGTTGGGCCT
TGATAATGAAACTTTAGAGACACTTTCTCAGCCCGAGAGAATAATTCAAGTTAAGATACAAATTAGGGGTTCTGACGGTA
AATTAAAGACATTTATGGGGTGGAGGAGTCAACACAACTCCGCCCTAGGTCCCTATAAGGGAGGAGTGAGATATCATCCT
AATGTTACGCAAGATGAGGTAGAAGCACTATCTATGATAATGACGTGGAAGAACTCGTTATTGTTATTGCCATATGGAGG
AGGAAAGGGTGGAGTTAGAGTAGACCCCAAAAAGTTAACTAGGGAAGAATTAGAACAATTATCCAGAAAGTACATTCAAG
CTATTTATAAATATTTGGGTAGTGAATTGGATATCCCAGCCCCAGATGTAAATACAGATTCACAAACCATGGCTTGGTTC
CTGGATGAGTACATAAAGATAACCGGAAAAGTTGATTTCGCAGTGTTCACTGGGAAGCCGGTAGAGCTGGGTGGAATAGG
GGTTAGGTTATATAGTACTGGACTTGGAGTTGCTACCATAGCTAAGGAAGCTGCAAATAAGTTTATAGGAGGGGTTGAGG
AGGCTAGAGTCATAATTCAAGGTTTTGGAAATGTTGGGTATTATGCAGGTAAGTTCCTAAGTGAAATGGGAGCTAAAATA
GTTGGAGTTAGCGATTCTAAAGGTGGAGTAATTAATGAGAAAGGAATAGATGTTGGAAAGGCTATTGAGATAAAGGAGAA
AACTGGAAGTGTGATAAATTATCCTGAGGGAAGGAAGGTAACCAATGAGGAATTGTTAATATCGGATTGTGATATATTAA
TTCCAGCTGCCCTTGAAAACGTCATAAACAAGTTCAATGCTCCGAAGGTTAAGGCAAAGCTTATTGTAGAAGGTGCAAAT
GGTCCGTTGACAGCAGATGCTGATGAAATAATGAGACAGAGGGGAATAGCAGTTGTACCAGATATACTCGCCAATGCTGG
TGGAGTTGTTGGGAGTTATGTGGAATGGGCTAATAATAAAATGGGAGAGATAATAAGCGATGAGGAAGCTAAGAAACTTA
TAGTCGATAGAATGAATAATGCCTTCAATACGCTATATGATTATCATCAGAAGAAGCTAGAAGATCATGACCTAAGAACT
GCTGCAATGGCATTGGCAGTAGACAGAGTAGTAAGAGCTATGAAAGCTAGGGGCATATTATAA

Upstream 100 bases:

>100_bases
TTTATATCGTTTTGACTTTTTCACCTAAAGAAAATTGTGAGTTAAGAAAATTTTAAAGCTGTGTTGGAATAAATTTTTAT
TCTGGGAAAACGAATTTATA

Downstream 100 bases:

>100_bases
CACTCTTTTTTATCCTTATCTCTTTCCTTTTACTCGTAACCTACTATTAAAAGTCTGTCCTTAAGCTTATAAGAATAAAG
AATTGTAAAATTAAAGCTAC

Product: NAD specific glutamate dehydrogenase (gdhA-2)

Products: NA

Alternate protein names: GDH-2

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGVRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLTREELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWF
LDEYIKITGKVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKI
VGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALENVINKFNAPKVKAKLIVEGAN
GPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNKMGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGIL

Sequences:

>Translated_420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGVRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLTREELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWF
LDEYIKITGKVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKI
VGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALENVINKFNAPKVKAKLIVEGAN
GPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNKMGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGIL
>Mature_420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGVRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLTREELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWF
LDEYIKITGKVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKI
VGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALENVINKFNAPKVKAKLIVEGAN
GPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNKMGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGIL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family

Homologues:

Organism=Homo sapiens, GI31377775, Length=372, Percent_Identity=38.4408602150538, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=39.5894428152493, Blast_Score=262, Evalue=6e-70,
Organism=Escherichia coli, GI1788059, Length=338, Percent_Identity=34.3195266272189, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=396, Percent_Identity=36.3636363636364, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324951, Length=396, Percent_Identity=29.7979797979798, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319256, Length=409, Percent_Identity=29.0953545232274, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24649283, Length=371, Percent_Identity=38.544474393531, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45549226, Length=339, Percent_Identity=38.3480825958702, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI45553475, Length=339, Percent_Identity=38.3480825958702, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=38.3512544802867, Blast_Score=201, Evalue=8e-52,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DHE2_SULSO (P80053)

Other databases:

- EMBL:   AE006641
- PIR:   D90355
- PIR:   S20286
- RefSeq:   NP_343309.1
- ProteinModelPortal:   P80053
- SMR:   P80053
- GeneID:   1453426
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1907
- NMPDR:   fig|273057.1.peg.1732
- HOGENOM:   HBG590661
- OMA:   YEGATEE
- ProtClustDB:   CLSK803108
- BioCyc:   SSOL273057:SSO1907-MONOMER
- BRENDA:   1.4.1.3
- GO:   GO:0005488
- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11606:SF2
- PIRSF:   PIRSF000185
- PRINTS:   PR00082
- SMART:   SM00839

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N

EC number: =1.4.1.3

Molecular weight: Translated: 46032; Mature: 46032

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHH
WRSQHNSALGPYKGGVRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLT
HHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHH
REELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWFLDEYIKITGKVDFAVFTGKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEECCC
VELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKI
EEECCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
VGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALEN
EEECCCCCCEECCCCCCCCCEEEEEHHCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHH
VINKFNAPKVKAKLIVEGANGPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNK
HHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEEHHHHHCCCCHHHHHHHHHCCC
MGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHH
WRSQHNSALGPYKGGVRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLT
HHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHH
REELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWFLDEYIKITGKVDFAVFTGKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEEECCC
VELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKI
EEECCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
VGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALEN
EEECCCCCCEECCCCCCCCCEEEEEHHCCCEEECCCCCCCCCCCEEEECCCCHHHHHHHH
VINKFNAPKVKAKLIVEGANGPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNK
HHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEEHHHHHCCCCHHHHHHHHHCCC
MGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726; 1730244