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The map label for this gene is pqqE-4 [H]

Identifier: 15898640

GI number: 15898640

Start: 1665410

End: 1666546

Strand: Direct

Name: pqqE-4 [H]

Synonym: SSO1839

Alternate gene names: 15898640

Gene position: 1665410-1666546 (Clockwise)

Preceding gene: 15898639

Following gene: 15898641

Centisome position: 55.66

GC content: 36.94

Gene sequence:

>1137_bases
ATGATTCCAATAAGCGTTCTAGTAACTAATGCTGGTACAGTTTCATTTCACATTAAGGGCGAGTATAATAGGGATAGACC
TAGTAAATTCAGTGACGTATTTAGACCAGTTGTAACGTGGAATCTCACAAGAAAATGCAACTTGAAATGTCTTCATTGTT
ATATTAACGCATCACCAGAAGGTGAGGATGGGTTAACTACAGAAGAGGCACTAGGGCTAATAGACGAGATGGCTGAAATG
AAGATCCCATTAATTATAATGAGTGGTGGAGAGCCTTTAATGAGAAGGGACTTCTTTGAATTGGCATCATATGCTAGGAG
TAAGGGAATAAAATTAGCCTTATCAACTAATGGAACATTGATAAGCGAGAGTGTGGCTAAGAGATTGAAGGAATTGGATT
TCTCTTACGTAGGTATAAGTTTAGATAGTTACGATCCGGAATTTCACGATAAGTTTAGAGGTCTAAATGGAGCCTTTAAC
ATGACAGTTAAGGGAATTGGAAATGCAATAAACGTTGGATTAAACGTTGGACTTAGATTCACTATTACGGCTAAAAACAT
CCATCAAGTAGACGAGTACATGAAATTGGCCTTGAAACTGGGAGTTAAGAGAATAACGTTTTACCATTTATCAGCCAGTG
GTAGGGGTAGGGAATTAAGGGAGTGGATGTATACTCTGGAGGAATATGTTAGTTTCATAAACAAAATGATTGATTATGCA
ATTAAGCTAAGTGGGAAGATAGAAATTGAGACTACACTAGGGCAATTTGATGGAGTTTACATTGCTAAAAAGTTAGCCAG
AAATGAGGAAGAACTTGAAAAATACCTGAAATTTGTCGAAAATAGTGGGGGATGTGGGAGGAAAATGATCTCAATATATC
CAAACGGTGACGTTTATCCTTGTCAATTCATTGACTTTTACAAGTTGGGTAACGTTAGGGAAAAACCGCTTAAGGAGATA
ATTAAGAATATTCCAGACTTGTTCATAAATACGGACAAGTATTTGAAAGGAAGGTATTGTGATAAGTGTGAATATAAATC
TGCTTGTAAAGGAGGAGATAGAGCTAGAGCGTATTATTGGAATGAAGACATTTACGGAGATGATCCGTTATGCCCTTTGA
AAACGCTCCACATTTAG

Upstream 100 bases:

>100_bases
AGGGTAAAAAGAGTCGTTGTGCAAAACAACCCCGTTTAAGAAAATAATTGCTAAAATATTAGGGATCGCTTTTTTATGAC
TAAGATGCCTTTTTATTATT

Downstream 100 bases:

>100_bases
TCTTCTGGGAAGTAACTAAAGCTTGTCCATTGAGTTGTAAACATTGTAGAGCTAATGCCATAGATAAGCCATTACCAGGG
GAGCTTAGTACTGAAGAAAG

Product: coenzyme PQQ synthesis protein E (pqqE-4)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGLTTEEALGLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTLISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIGNAINVGLNVGLRFTITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYWNEDIYGDDPLCPLKTLHI

Sequences:

>Translated_378_residues
MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGLTTEEALGLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTLISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIGNAINVGLNVGLRFTITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYWNEDIYGDDPLCPLKTLHI
>Mature_378_residues
MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGLTTEEALGLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTLISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIGNAINVGLNVGLRFTITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYWNEDIYGDDPLCPLKTLHI

Specific function: Required for production of the bacteriocin subtilosin. May activate a metalloenzyme that catalyzes a modification of prosubtilosin [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786999, Length=206, Percent_Identity=24.7572815533981, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR008792
- InterPro:   IPR007197 [H]

Pfam domain/function: PF05402 PqqD; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43029; Mature: 43029

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
GEDGLTTEEALGLIDEMAEMKIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
ISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFNMTVKGIGNAINVGLNVGLRF
HHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCEEEEEEECCEEE
TITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYW
EHEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCCCCCCCHHHHCCCCCCCEEEEE
NEDIYGDDPLCPLKTLHI
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
GEDGLTTEEALGLIDEMAEMKIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
ISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFNMTVKGIGNAINVGLNVGLRF
HHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCEEEEEEECCEEE
TITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYW
EHEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCCCCCCCHHHHCCCCCCCEEEEE
NEDIYGDDPLCPLKTLHI
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 10572140; 10809709; 10809710 [H]