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The map label for this gene is mcrB [H]

Identifier: 15898169

GI number: 15898169

Start: 1159034

End: 1160713

Strand: Reverse

Name: mcrB [H]

Synonym: SSO1328

Alternate gene names: 15898169

Gene position: 1160713-1159034 (Counterclockwise)

Preceding gene: 15898170

Following gene: 15898168

Centisome position: 38.79

GC content: 32.32

Gene sequence:

>1680_bases
GTGTATGGTGATTGGTTAGTAGTCAGAGACAAGTTCAAAAAATTACTAGACTCTCAAGATAGTGCGCAAACTTTTCTCGA
TTATATTGAAAAAGGAAATTTTGTTCCTTGCTTCTTTATAGGTGAAAAATCTCATTGGGTAGAATCTATAAAATATTCTT
TTTATGGAGAATTAGGATATACTTTATGGGGTGCTTCGGCTGAAAATCGTAGCAATATTAGTAGCATTTTAAACAGGTAT
GACGAAATCTATTTTGGAGAAGATAAGAAGAGAAAAGATAAGAAAAATGATACAGAGAATAAAAATAAAATAGAAATTAA
GAACGATAAAATACTGGTAGCCCTTCTAAGAACAAAAGAGCTAGGCATTATTGGATTCGGAGTAGTAACGGATATCACAA
AGGACGCGATGAGGAATTTTAAAGGATGGAAGGAAGGTGACAAAGTCTGGATAGTAAGATTTAGAATAAAAATATTTTGG
CTTCACGAAAGCATAAGAAAAAACTATAATGACAGTAATAAATGGGTGGGAGAAGATTATAAGTTAGAAGGGGTTAATAA
TCAATCAAACCAATGCTATACAGAAGACAATAATGATGCAGTTAAAGCTTTTCAACAATTTATTCTATCGAAGAAAGAGG
AAATTAGGTCTACTTTAGAATTTTATTCTAAGTTTCGTTTCAGTACTCAGAAAGAATACCTATCAGAGGAAGTAGTTTGT
AATAAAAACGCACAAATAGATCTTAATGGTTTCTATATTCCTCAAGATTCAGTTAACATTATACTGAAAGCTCTACAAAC
TACTAATGTACTCCTAGCTGGCCCACCTGGTACTGGAAAAACTAGCTTAGCTATAAGGACTGTGAGAGCTTTAACTGGTA
ATAATGATAACTGTTATGAAGTAGCTACTGCCAATTCACTTTGGTTTAGAAGGAATTTGATAGGAGGAGAAAGTATAAGA
GAGGGATCTGTAATGTGGAAGAGCGGGCTTTTCATTGAAGCTTACGTAAATGCTGCAAGAATAAAGGATGGGAACTATTA
CGTCGTAATTGATGAATTGAATAGGGCTGACGTTGATAAGGCATTTGGAGAATTAATGACAATATTTTCTACTCCATATC
CTAGCGAATGGTCAATTCCCAATGCTCTAATAGAGGAAATTAAGAGTTATGGAGATAATATAGATAATGTAACCAAGAGT
TTCTTAGAAATATATGAGAAGTTAAAGAGGGAGAGTAGAGAGAATGAACCATTAAAAAGAATACGAATTATTGCTACAAT
AAACTTAGTCGATGCTAGAAATCTGTTTTATGTTGGAGATGCGCTGGCAAGAAGGTTCGTGATAATTTACTTTGATTATC
CGAAAGAAACCGAAGATTTAGATGAATTTCTTAAAACTTACGCTTCTTTTAGTGAGGACTTTAAGAATAAAGTTAAGGAT
CTTGTAAAATATCTTAGAGTTAATCTAAATGAAAAAGAAGATAAGGTAATAAAGTTTAATATCTCACCGGCTAGCTTAAA
GACAGCGCTGGATATATATTCCCGTCTTGATGACAACAATTCTGATATATATAAGTTCGTTGAAGTTTTAAGATCCACTA
TAGGGACACTAAATCCAGATAATATAAATAAATTTAATAGATTAATAGAAAAATGGAAAAAAGAAAAAGGAGGACAATAA

Upstream 100 bases:

>100_bases
GATTATCATCGATCCTTTGGAGTCAGATGAAAATAATTTATCGAAGATCGATAAGATAATGAAAGAACACATTTAAATAC
AAGACTACCTACCCTTTTTT

Downstream 100 bases:

>100_bases
AAAAGTTAATTCACTTCTTGTTCTTGCTTTCATCTTCCTTTAACTCTTCTACTATCTCCCTCATTATCCTCTCTGAAGGA
AATGACACCACTACTCCCTT

Product: restriction endonuclease related protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MYGDWLVVRDKFKKLLDSQDSAQTFLDYIEKGNFVPCFFIGEKSHWVESIKYSFYGELGYTLWGASAENRSNISSILNRY
DEIYFGEDKKRKDKKNDTENKNKIEIKNDKILVALLRTKELGIIGFGVVTDITKDAMRNFKGWKEGDKVWIVRFRIKIFW
LHESIRKNYNDSNKWVGEDYKLEGVNNQSNQCYTEDNNDAVKAFQQFILSKKEEIRSTLEFYSKFRFSTQKEYLSEEVVC
NKNAQIDLNGFYIPQDSVNIILKALQTTNVLLAGPPGTGKTSLAIRTVRALTGNNDNCYEVATANSLWFRRNLIGGESIR
EGSVMWKSGLFIEAYVNAARIKDGNYYVVIDELNRADVDKAFGELMTIFSTPYPSEWSIPNALIEEIKSYGDNIDNVTKS
FLEIYEKLKRESRENEPLKRIRIIATINLVDARNLFYVGDALARRFVIIYFDYPKETEDLDEFLKTYASFSEDFKNKVKD
LVKYLRVNLNEKEDKVIKFNISPASLKTALDIYSRLDDNNSDIYKFVEVLRSTIGTLNPDNINKFNRLIEKWKKEKGGQ

Sequences:

>Translated_559_residues
MYGDWLVVRDKFKKLLDSQDSAQTFLDYIEKGNFVPCFFIGEKSHWVESIKYSFYGELGYTLWGASAENRSNISSILNRY
DEIYFGEDKKRKDKKNDTENKNKIEIKNDKILVALLRTKELGIIGFGVVTDITKDAMRNFKGWKEGDKVWIVRFRIKIFW
LHESIRKNYNDSNKWVGEDYKLEGVNNQSNQCYTEDNNDAVKAFQQFILSKKEEIRSTLEFYSKFRFSTQKEYLSEEVVC
NKNAQIDLNGFYIPQDSVNIILKALQTTNVLLAGPPGTGKTSLAIRTVRALTGNNDNCYEVATANSLWFRRNLIGGESIR
EGSVMWKSGLFIEAYVNAARIKDGNYYVVIDELNRADVDKAFGELMTIFSTPYPSEWSIPNALIEEIKSYGDNIDNVTKS
FLEIYEKLKRESRENEPLKRIRIIATINLVDARNLFYVGDALARRFVIIYFDYPKETEDLDEFLKTYASFSEDFKNKVKD
LVKYLRVNLNEKEDKVIKFNISPASLKTALDIYSRLDDNNSDIYKFVEVLRSTIGTLNPDNINKFNRLIEKWKKEKGGQ
>Mature_559_residues
MYGDWLVVRDKFKKLLDSQDSAQTFLDYIEKGNFVPCFFIGEKSHWVESIKYSFYGELGYTLWGASAENRSNISSILNRY
DEIYFGEDKKRKDKKNDTENKNKIEIKNDKILVALLRTKELGIIGFGVVTDITKDAMRNFKGWKEGDKVWIVRFRIKIFW
LHESIRKNYNDSNKWVGEDYKLEGVNNQSNQCYTEDNNDAVKAFQQFILSKKEEIRSTLEFYSKFRFSTQKEYLSEEVVC
NKNAQIDLNGFYIPQDSVNIILKALQTTNVLLAGPPGTGKTSLAIRTVRALTGNNDNCYEVATANSLWFRRNLIGGESIR
EGSVMWKSGLFIEAYVNAARIKDGNYYVVIDELNRADVDKAFGELMTIFSTPYPSEWSIPNALIEEIKSYGDNIDNVTKS
FLEIYEKLKRESRENEPLKRIRIIATINLVDARNLFYVGDALARRFVIIYFDYPKETEDLDEFLKTYASFSEDFKNKVKD
LVKYLRVNLNEKEDKVIKFNISPASLKTALDIYSRLDDNNSDIYKFVEVLRSTIGTLNPDNINKFNRLIEKWKKEKGGQ

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=255, Percent_Identity=29.8039215686275, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 64938; Mature: 64938

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGDWLVVRDKFKKLLDSQDSAQTFLDYIEKGNFVPCFFIGEKSHWVESIKYSFYGELGY
CCCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCC
TLWGASAENRSNISSILNRYDEIYFGEDKKRKDKKNDTENKNKIEIKNDKILVALLRTKE
EEECCCCCCHHHHHHHHHHHHHEEECCCHHHCCCCCCCCCCCEEEEECCEEEEEEEECCC
LGIIGFGVVTDITKDAMRNFKGWKEGDKVWIVRFRIKIFWLHESIRKNYNDSNKWVGEDY
CCEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEHHHHCCCCCCCCCCCCCE
KLEGVNNQSNQCYTEDNNDAVKAFQQFILSKKEEIRSTLEFYSKFRFSTQKEYLSEEVVC
EEECCCCCCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
NKNAQIDLNGFYIPQDSVNIILKALQTTNVLLAGPPGTGKTSLAIRTVRALTGNNDNCYE
CCCCEEEECEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEE
VATANSLWFRRNLIGGESIREGSVMWKSGLFIEAYVNAARIKDGNYYVVIDELNRADVDK
EEECCCHHHHHHCCCCCCCCCCCEEEECCCEEEEECCHHEEECCCEEEEEECCCCCHHHH
AFGELMTIFSTPYPSEWSIPNALIEEIKSYGDNIDNVTKSFLEIYEKLKRESRENEPLKR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
IRIIATINLVDARNLFYVGDALARRFVIIYFDYPKETEDLDEFLKTYASFSEDFKNKVKD
EEEEEEEEEECCCCEEEECHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVKYLRVNLNEKEDKVIKFNISPASLKTALDIYSRLDDNNSDIYKFVEVLRSTIGTLNPD
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NINKFNRLIEKWKKEKGGQ
CHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYGDWLVVRDKFKKLLDSQDSAQTFLDYIEKGNFVPCFFIGEKSHWVESIKYSFYGELGY
CCCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCC
TLWGASAENRSNISSILNRYDEIYFGEDKKRKDKKNDTENKNKIEIKNDKILVALLRTKE
EEECCCCCCHHHHHHHHHHHHHEEECCCHHHCCCCCCCCCCCEEEEECCEEEEEEEECCC
LGIIGFGVVTDITKDAMRNFKGWKEGDKVWIVRFRIKIFWLHESIRKNYNDSNKWVGEDY
CCEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEHHHHCCCCCCCCCCCCCE
KLEGVNNQSNQCYTEDNNDAVKAFQQFILSKKEEIRSTLEFYSKFRFSTQKEYLSEEVVC
EEECCCCCCCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
NKNAQIDLNGFYIPQDSVNIILKALQTTNVLLAGPPGTGKTSLAIRTVRALTGNNDNCYE
CCCCEEEECEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEE
VATANSLWFRRNLIGGESIREGSVMWKSGLFIEAYVNAARIKDGNYYVVIDELNRADVDK
EEECCCHHHHHHCCCCCCCCCCCEEEECCCEEEEECCHHEEECCCEEEEEECCCCCHHHH
AFGELMTIFSTPYPSEWSIPNALIEEIKSYGDNIDNVTKSFLEIYEKLKRESRENEPLKR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
IRIIATINLVDARNLFYVGDALARRFVIIYFDYPKETEDLDEFLKTYASFSEDFKNKVKD
EEEEEEEEEECCCCEEEECHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVKYLRVNLNEKEDKVIKFNISPASLKTALDIYSRLDDNNSDIYKFVEVLRSTIGTLNPD
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NINKFNRLIEKWKKEKGGQ
CHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]