Click here to switch to the map view.

The map label for this gene is thiC-1

Identifier: 15898166

GI number: 15898166

Start: 1153789

End: 1155090

Strand: Reverse

Name: thiC-1

Synonym: SSO1324

Alternate gene names: 15898166

Gene position: 1155090-1153789 (Counterclockwise)

Preceding gene: 15898168

Following gene: 15898165

Centisome position: 38.6

GC content: 41.55

Gene sequence:

>1302_bases
ATGGCCATAATAGAGGAAGCAAGAAGTGGCCAAATAACAGACGAGATGAAGGAAATTAGTAAGCTTGAGGGAATTCCAGT
AGAGAAGGTGCGAAATAGGATAAGTGAGTGTAAAATAATCCTAATTAGGAACGCTAAGTATCCTAGCAAAAAGTTGGTGC
CAATTGGTAAAGGGTTGACCACTAAAGTTAACATAAACATTGGAACGTCGGATGAAGTTGTAAATCTACAAATGGAGTTG
GAGAAGGTAAAGGTTGCTAATAGGTGGGGAGACACACTAATGGATCTATCGACTGGAGGTGATTTAGATGACATAAGGAG
GGAGATAATAAGGGCGTCTGAATTGCCTGTTGGTACAGTACCAGTTTATCAAGTTTTCATAGAGTCCTTTAAGAAGAAAT
CTGGTGGTGCGTATTTCACTGAGGATGAATTACTGAGTACTGTGGAGAAGCACTTAAAGGATGGAGTAGCCTTTATGACA
ATTCACGCTGGGATAACTAAGGAATTAGCCATAAGGGCGTTGAAGAGCAATAGGATAATTCCAATAGTATCAAGGGGAGG
GGATATGATAGCTGGTTGGATGATACACAATAATTCTGAAAATCCATATAGGAAGCACTGGGGCTACTTACTGGAGATGT
TTAAGGAATATGACGCAGTGATTTCATTAGGTGATGCATTGAGACCTGGAGCAACGGGGGATGCTCATGATGAATTTCAA
GTTGGTGAACTTTTGGAAACGGCCAGACTGGTTAAAAGTGCGTTAAAGGAGGGTGTGCAAGTAATGGTAGAAGGTCCGGG
ACACGTACCATTAAATGAAATAGCATGGGATGTGAAATTGATGAAGAAGTTAACTGGTGGTGTACCATATTATGTTTTAG
GTCCTTTGCCGATAGATGTCGGTGCACCATACGATCACATAGCCTCTGCAATAGGTGCTGCAATATCATCAGCTGCTGGT
GCAGATCTATTGTGCTATTTGACTCCAGCCGAACATTTGGGATTGCCAACTGTTAAACAAGTTGAGGAAGGGGCAATTGC
TTACAGAATTGCAGCTCACGCTGGTGATGTGGTTAAGTTAGGGAGAAGAGTTAGGAAATGGGATGATGAGGTTAGCTATT
ATAGGGGTAAGTTAGAGTGGGATAATATGATTTCTAGGCTCATAGATCCCCAAAGAGCTTATCAAGTCTACACTCAGTTT
GGTATTCCGAAGGTTAAAGCGTGTACTATGTGTGGTGGATATTGTCCAATGATGTGGGCAATGGATCAAGTGAGGAAGAT
AGGTTCTTCTTCATCCTTATAA

Upstream 100 bases:

>100_bases
ACGTAGTGATGATTCAAATTGAATAGTTCTAGCTACTAGGAGATTCTCCCATCTTATAGTATATAGTAAGGTTTATAACT
CACTTATATGAATATTTCTT

Downstream 100 bases:

>100_bases
GTACGGATTCATATTTTTATTTTCGTACTCCAGTACTTTTTTATGGATAAAACAGTTTTAGATGCGAATTTAGATCCGTT
AAAGGGAAAGACAATAGGTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLTTKVNINIGTSDEVVNLQMEL
EKVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTVPVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMT
IHAGITKELAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQ
VGELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSAAG
ADLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQF
GIPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL

Sequences:

>Translated_433_residues
MAIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLTTKVNINIGTSDEVVNLQMEL
EKVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTVPVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMT
IHAGITKELAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQ
VGELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSAAG
ADLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQF
GIPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL
>Mature_432_residues
AIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLTTKVNINIGTSDEVVNLQMELE
KVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTVPVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMTI
HAGITKELAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQV
GELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSAAGA
DLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQFG
IPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=420, Percent_Identity=37.8571428571429, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC1_SULSO (Q97YJ8)

Other databases:

- EMBL:   AE006641
- PIR:   B90288
- RefSeq:   NP_342771.1
- ProteinModelPortal:   Q97YJ8
- GeneID:   1454340
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1324
- NMPDR:   fig|273057.1.peg.1194
- HOGENOM:   HBG285234
- OMA:   RGKLEWD
- ProtClustDB:   PRK13352
- BioCyc:   SSOL273057:SSO1324-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47832; Mature: 47701

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLT
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEECCCCCCCCCEECCCCCE
TKVNINIGTSDEVVNLQMELEKVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTV
EEEEEEECCCCCEEEEHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
PVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMTIHAGITKELAIRALKSNRII
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VGELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAISSAAGADLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKL
CCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHCCCEEEEEHHCCCHHHHH
GRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQFGIPKVKACTMCGGYCPMMWA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHH
MDQVRKIGSSSSL
HHHHHHCCCCCCC
>Mature Secondary Structure 
AIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLT
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEECCCCCCCCCEECCCCCE
TKVNINIGTSDEVVNLQMELEKVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTV
EEEEEEECCCCCEEEEHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
PVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMTIHAGITKELAIRALKSNRII
HHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCEE
PIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQ
EEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VGELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDV
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECC
GAPYDHIASAIGAAISSAAGADLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKL
CCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHCCCEEEEEHHCCCHHHHH
GRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQFGIPKVKACTMCGGYCPMMWA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHH
MDQVRKIGSSSSL
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726