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The map label for this gene is todA [H]

Identifier: 15898077

GI number: 15898077

Start: 1064502

End: 1065716

Strand: Direct

Name: todA [H]

Synonym: SSO1225

Alternate gene names: 15898077

Gene position: 1064502-1065716 (Clockwise)

Preceding gene: 15898076

Following gene: 15898078

Centisome position: 35.58

GC content: 37.12

Gene sequence:

>1215_bases
ATGAAATGTGAATACTTAATTATTGGAAGTGGCATTGCGGGATATAACGCACTAAAGGAATTACTCCAAATAAAACCAAA
CTCGAGAATAATTATGATAACCTCAGACAAATATTATCCATATGATAGACCACCACTATCAAAGGATTATCTTAAAGGAA
AATTGGAAAAAGATATGTTATTCTTCGAGTCTGATGATTTTTACAAGAGGGATAATTTAGAGGTAATGTTAAACAAGAGT
GTCGAAAGGATTGATGCTAATTTAAAGGAGGCAATACTAAATGATGGTAGTGTAATATCCTTTGATAAGGCATTAATAAG
TACTGGTGGTAGGCCTAGGAGATTAAACATTCCGGGGAGTGAAAACGCGTTATACTTAAGAAGTTTGGATGACGCAGATA
GGATAAGGGAAGCTGCGAGTAAAGGCAAAAACGCGTTAATAATTGGTGCTGGATTTATAGGTGTTGAAGTGGCCTCTAGC
TTGATAACACTTGGTGTAAAGACTACAGTAGTGGAAGTAATGCCATACATATGGAATACATTCGTAGACGAAAAAGTATC
AAGAGTCATACAACAATACTTGGAAAGTAAGGGAATAAACTTCATTCTAAACGAGTCAGTGAAGGAGATTCAAGGGAAAA
TCGCAACAACCTCAAGCGGTAGAAAAATTGAGGCAGACATGTTCCTAATCGCAGTGGGAATCTCGCCAAATGTCGAACTA
GCGCAGAGAAGTGGAATGCAAGTGGATAATGGCATAGTTGTTAACGAGTACCTAGAGACGAGTGCCAGGGATATTTATGC
AGCTGGGGACATCGCTAACATATTCGATCCAAGGGAGGGTAAGAGGAAGAGAATAGAGCATTGGAACAACGCGGAATACA
CTGGAAAATTGGCAGCTAGGAATATGGCTGGAAGCAGAGAGGCTTACAATTTCATATCATCCATATGGTCAGACATATTC
GACTTACACATAGAGTCAGCCGGTGAAACAAGGAATTATGACGAATATATAATAAGGGGAAAGTTTGAATTGGAAAGACC
AAGGTTTAGTGTAATATACTTAAAAGGAGGTATTATAAAGGGTTACTTAGCGATAAATAGAAACGTTAAAGAAATAATAG
CCCTCAATAAATTAATACAAAAGCAAGCAGACGTTTCAAGTAAAAGAGATAAGTTGGCAGATGAAAGTTTTGATCTTCAG
AAACTTTTAATATAA

Upstream 100 bases:

>100_bases
ATCTTCCTTTTTTTAAATATAGACTTTTAAGTTCAAAATCTCCATGCATCAAACTTGTTAGATTAAGCTTAATATAGCTT
TCAACGGTTCATGAATGTAG

Downstream 100 bases:

>100_bases
GTGGGATTTAAAGTTACTTCTTTTTTCGAACATGTTTAAGAATTATTTTTAAATGAGGATTATACATGTTTACTTATGAA
ACCAGTTCCTGTAGAGCCTA

Product: toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component, (todA)

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDMLFFESDDFYKRDNLEVMLNKS
VERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGSENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASS
LITLGVKTTVVEVMPYIWNTFVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVEL
AQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAARNMAGSREAYNFISSIWSDIF
DLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIKGYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQ
KLLI

Sequences:

>Translated_404_residues
MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDMLFFESDDFYKRDNLEVMLNKS
VERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGSENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASS
LITLGVKTTVVEVMPYIWNTFVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVEL
AQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAARNMAGSREAYNFISSIWSDIF
DLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIKGYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQ
KLLI
>Mature_404_residues
MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDMLFFESDDFYKRDNLEVMLNKS
VERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGSENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASS
LITLGVKTTVVEVMPYIWNTFVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVEL
AQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAARNMAGSREAYNFISSIWSDIF
DLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIKGYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQ
KLLI

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=392, Percent_Identity=28.5714285714286, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI226437568, Length=392, Percent_Identity=28.5714285714286, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI65787454, Length=392, Percent_Identity=28.5714285714286, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI4757732, Length=334, Percent_Identity=30.2395209580838, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI22202629, Length=334, Percent_Identity=30.2395209580838, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI22202631, Length=161, Percent_Identity=33.5403726708075, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI195927004, Length=103, Percent_Identity=41.747572815534, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI195927006, Length=165, Percent_Identity=28.4848484848485, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788892, Length=405, Percent_Identity=27.6543209876543, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789065, Length=348, Percent_Identity=26.1494252873563, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789765, Length=281, Percent_Identity=28.1138790035587, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082354, Length=337, Percent_Identity=25.5192878338279, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789915, Length=224, Percent_Identity=28.125, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786307, Length=230, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=326, Percent_Identity=27.3006134969325, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564386, Length=346, Percent_Identity=28.3236994219653, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565766, Length=142, Percent_Identity=31.6901408450704, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573993, Length=363, Percent_Identity=32.5068870523416, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24581020, Length=363, Percent_Identity=32.5068870523416, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24585130, Length=356, Percent_Identity=28.3707865168539, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24639257, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281359715, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281359713, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639250, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18543267, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639252, Length=352, Percent_Identity=29.2613636363636, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 45526; Mature: 45526

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDML
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCHHHCEE
FFESDDFYKRDNLEVMLNKSVERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGS
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCEEEECCCC
ENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASSLITLGVKTTVVEVMPYIWNT
CCEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVEL
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHCCCEEECCCCCEEEEEEEEEEECCCCCHHH
AQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAAR
HHHCCCCCCCCEEEHHHHHCCCCCEEECCCHHHHCCCCCCHHHHHHCCCCCCEEHHHHHH
NMAGSREAYNFISSIWSDIFDLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCEEE
GYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQKLLI
EEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDML
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCHHHCEE
FFESDDFYKRDNLEVMLNKSVERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGS
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCEEEECCCC
ENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASSLITLGVKTTVVEVMPYIWNT
CCEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVEL
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHCCCEEECCCCCEEEEEEEEEEECCCCCHHH
AQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAAR
HHHCCCCCCCCEEEHHHHHCCCCCEEECCCHHHHCCCCCCHHHHHHCCCCCCEEHHHHHH
NMAGSREAYNFISSIWSDIFDLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIK
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCEEE
GYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQKLLI
EEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]