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The map label for this gene is lplA-like2 [H]

Identifier: 15897974

GI number: 15897974

Start: 959676

End: 960710

Strand: Direct

Name: lplA-like2 [H]

Synonym: SSO1112

Alternate gene names: 15897974

Gene position: 959676-960710 (Clockwise)

Preceding gene: 15897971

Following gene: 15897975

Centisome position: 32.07

GC content: 37.49

Gene sequence:

>1035_bases
ATGAGAGAATTAAGGTTCTTAATTGAACGAAATAGTCAAGCTCATATACTAGCAGGTGAAGAAGCTCTTCTTTTATCAGT
CGCTAATGGTTCCCAACCAATTTTACGATTTGTAATATTTGATCCTCCAGCAGTTCTTGTAGGCTATCATCAAGCGGTTG
AGCAAGAAGTAAATATAGAAGAGGTGAAAAAAAGAGGCTGGGAAATAGGTAGAAGGCCTACTGGGGGAGGTACAATTATA
ATGGGCCCATGGCAATTGGGTTGGGAAATCTACGCAAACAATGATCTATTAGGGTATACTCCAGAAAATGCAATAAAAAT
TGGTGCTGAAGGCGTTATTAGGGCACTAGACAAGTTGGGTATAAAAGCATCTTTTAGGCCAAAGAATGATGTAGAGGTAA
ACGGGAGGAAGATTTCTGGAATCGGAGCATTTTCTGAGGGTAATTACATTGCAGTTACCGGAACAATTCTACTAGATTTT
GATGTGGACGCGATGATTTCAGTTCTTAGGTTATCTTCTGAAAAACTTAAGGACAAACTAGCCAGAGATTTTAAGGACCG
ATTAACGTGGGTTAATAAGGAATTAAAACGCTCACTTGATATGGAAGAATTAATAAAAATCTCTAGAGATTCCTTTGCTG
AAGTGCTAGGGATAAAGCTTTCGGATGACTACTACAATGAATTTGAAAAGAAGACCATACAAGAATTAGCGTTAAAGTAT
TCATCGCCCGAATGGATATTTAACTTAAGGCGACCATTAATTGGGGACGATATAAGATACGTAGAAAGGAAACTTCCAGG
TGGACTGGTTAAGGTGCAAGTTAAAATGGTCAGTAAAAATCTAATAGAATCTGTACTAGTAACTGGTGATTTCTTCATAG
AACCGAGAACTGCCATATATGATTTGGAAGCTAGATTGAAATGGAGTAGAGTTGAAGATTTAGAAAACGAAATCAGAACG
TGGTTTAACAGTGTGAAGGCAATTGGAATAACTGCTGATGATCTCATAAAAATAATAGAAGAGGTGGTAAGGTGA

Upstream 100 bases:

>100_bases
ACTAGTATTTTTTGGCTTTTAAATTTTTCTATATCAATTTAATGTTTTTCGAGGATTTGAGAGAAATATTTTTATATTAA
GTTAACTAAAGCATTTAGGA

Downstream 100 bases:

>100_bases
GGCCATTATTCCTTTATGCTCCCAATTTAAAGAGGTATGAGAGTGAGTTTTTAGATTCTAGAAAAGGTTGGAAATCAATA
TCTGTTACGGGTACTTATTG

Product: lipoate protein ligase A

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIEEVKKRGWEIGRRPTGGGTII
MGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLGIKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDF
DVDAMISVLRLSSEKLKDKLARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKY
SSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIYDLEARLKWSRVEDLENEIRT
WFNSVKAIGITADDLIKIIEEVVR

Sequences:

>Translated_344_residues
MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIEEVKKRGWEIGRRPTGGGTII
MGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLGIKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDF
DVDAMISVLRLSSEKLKDKLARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKY
SSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIYDLEARLKWSRVEDLENEIRT
WFNSVKAIGITADDLIKIIEEVVR
>Mature_344_residues
MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIEEVKKRGWEIGRRPTGGGTII
MGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLGIKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDF
DVDAMISVLRLSSEKLKDKLARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKY
SSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIYDLEARLKWSRVEDLENEIRT
WFNSVKAIGITADDLIKIIEEVVR

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 39110; Mature: 39110

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIE
CCHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHH
EVKKRGWEIGRRPTGGGTIIMGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLG
HHHHCCHHCCCCCCCCCEEEECCCEECEEEEECCCEEEECCCCCEEECHHHHHHHHHHHC
IKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDFDVDAMISVLRLSSEKLKDKL
CEEECCCCCCCEECCEEEEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
ARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHC
SSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIY
CCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEECCCHHEE
DLEARLKWSRVEDLENEIRTWFNSVKAIGITADDLIKIIEEVVR
EHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHC
>Mature Secondary Structure
MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIE
CCHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHH
EVKKRGWEIGRRPTGGGTIIMGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLG
HHHHCCHHCCCCCCCCCEEEECCCEECEEEEECCCEEEECCCCCEEECHHHHHHHHHHHC
IKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDFDVDAMISVLRLSSEKLKDKL
CEEECCCCCCCEECCEEEEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
ARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHC
SSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIY
CCCHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEECCCHHEE
DLEARLKWSRVEDLENEIRTWFNSVKAIGITADDLIKIIEEVVR
EHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]