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The map label for this gene is cysA-1 [C]

Identifier: 15897887

GI number: 15897887

Start: 878058

End: 878894

Strand: Direct

Name: cysA-1 [C]

Synonym: SSO1019

Alternate gene names: 15897887

Gene position: 878058-878894 (Clockwise)

Preceding gene: 15897882

Following gene: 15897895

Centisome position: 29.34

GC content: 37.63

Gene sequence:

>837_bases
TTGTTCTTTCATATTATGCTTGTAAGTGTTGAATGGCTTAACAACAACTTAAAGGACGTTAAGGTTGTAGAGATTGGTTA
TAATCCGCAGATAAATTATTATGAGGGGCATATTCCGGGGGCAGTATTAATAAATTGGAGAGATTTTCTTGACGATAATT
CTAGAGATTTCGCTAGTCCGGGGAAGTTGAGTAAGGTTTTAGGAAACGCGGGGATAAACAACGATGATCTCATTGTGTTA
TATAGCGAAATGAATAATAGGTACGCATTTTACGTTTATTGGATTTTAAAGGCTTATGGACATTCAAATTTGGCTATATT
AAATGGGGGAATTTATAAGTGGTTAAAAGAGGGTTATCCTATAGAAAGCGATGGTATAGTAGTTAATAAGAAGAGTGAAT
ACAAGGCTAGGAAGCCTGATTGGTCCTCAAGGATTTTAGTATGGGAGTTATTGTCTAAACTTAAAGAGTTTGTTTTAATC
GACTCTAGGAGTAAGGAGGAGTATGATGGTCTGACTACTGCACCTCCAGAGCACAAGTGTGAACAAACCCAAATGAGTGG
TCACATTCCGGGTGCTAAGAACATTCCATGGACAATTTTGCTTAATGAAGATGAGACTATGAAATCTAGGGATGAGTTGG
AGAGAATATTTTCTTGGTTAAGTAAAGAGGATAAGATAGTGGTGTACTGTAGGACTGGTGCTAGGGCTTCTGTTGCTTGG
TATGCTTTAAAAGAGGTTTTGGGATTTAAGTTGGTCAGACTTTATGATGGTTCATGGGTTGAATATGGCAATATGGTTGG
TGTCCCAGTAGAGAAATCAGTTAGTGATCGTTTCTGA

Upstream 100 bases:

>100_bases
TTCATGGTCTTGTGTAAGTTATAGTACAAGTTTTACTGGTTTCAATAACCTAAAATGCTGTAAATTGTAGAATATTTGAT
TAAAAGCTTATTAACCTAAA

Downstream 100 bases:

>100_bases
TCTTCATTTTCTCTCAAGGTATTTACAAAATAAAATGTCTAACTCTTTATACAGTTCTTCTGCTAATGCCTTGTCGTAGT
ATTTGCAATTGGCTATTAGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASPGKLSKVLGNAGINNDDLIVL
YSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYPIESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLI
DSRSKEEYDGLTTAPPEHKCEQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAW
YALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF

Sequences:

>Translated_278_residues
MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASPGKLSKVLGNAGINNDDLIVL
YSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYPIESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLI
DSRSKEEYDGLTTAPPEHKCEQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAW
YALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF
>Mature_278_residues
MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASPGKLSKVLGNAGINNDDLIVL
YSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYPIESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLI
DSRSKEEYDGLTTAPPEHKCEQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAW
YALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=300, Percent_Identity=30.6666666666667, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI194473668, Length=283, Percent_Identity=28.9752650176678, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI61835204, Length=283, Percent_Identity=28.9752650176678, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194473681, Length=283, Percent_Identity=28.9752650176678, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=28.9285714285714, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081967, Length=262, Percent_Identity=26.7175572519084, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=302, Percent_Identity=28.476821192053, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71997283, Length=298, Percent_Identity=26.8456375838926, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=214, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17559150, Length=259, Percent_Identity=24.7104247104247, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=250, Percent_Identity=27.2, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32080; Mature: 32080

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASP
CEEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEECCCCCEEEEEEHHHCCCCCCCCCCC
GKLSKVLGNAGINNDDLIVLYSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYP
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEHHHCCCCEEEEECHHHHHHHCCCC
IESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLIDSRSKEEYDGLTTAPPEHKC
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHCCCCCCCCCHHH
EQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAW
HHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
YALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF
HHHHHHHCEEEEEEECCCEEECCCEECCCCCCCCCCCC
>Mature Secondary Structure
MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASP
CEEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEECCCCCEEEEEEHHHCCCCCCCCCCC
GKLSKVLGNAGINNDDLIVLYSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYP
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEHHHCCCCEEEEECHHHHHHHCCCC
IESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLIDSRSKEEYDGLTTAPPEHKC
CCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHCCCCCCCCCHHH
EQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAW
HHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
YALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF
HHHHHHHCEEEEEEECCCEEECCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA