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The map label for this gene is leuA-2

Identifier: 15897857

GI number: 15897857

Start: 835585

End: 836970

Strand: Direct

Name: leuA-2

Synonym: SSO0977

Alternate gene names: 15897857

Gene position: 835585-836970 (Clockwise)

Preceding gene: 15897856

Following gene: 15897866

Centisome position: 27.93

GC content: 37.23

Gene sequence:

>1386_bases
ATGATAAAAGTAGGTATTTTAGATTCGACGTTAAGAGAAGGAGAACAAACTCCTGGAGTAATATTTACTGTAGACCAAAG
AGTAGAGATAGCTAAGGCTCTATCCGATTTAGGAGTATCTATGATAGAAGCCGGTCATCCGGCTGTATCTCCAGATATTT
ACGAAGGGATAAAAAGAATAGTCAAATTGAAAAAAGAGGGTATTATAACATCAGAAATTGTAGGACACAGTAGAGCCGTA
AAAAGAGATATAGAAATTGCAGCAGAATTAGAGGTAGATAGGATAGCAATATTTTACGGCGTAAGTGATATACATCTAAA
GGCGAAACATAAAGCAACAAGAGAAGAGGCTTTAAGGGTAATAGCTGAGACAATTAGTTACGCTAGGAGTCACGGCGTAA
AAGTCAGATTTACCGCAGAAGATGGTTCAAGGACAGACTTTGACTTCTTAGTTACAGTATCGAGAACGGCTAGAGATGCA
GGTGCGGATAGGGTTAGTATAGCTGATACTGTAGGCATATTATATCCATCAAAAACCAAGGAATTATTTAGCGCGTTAAT
AAGGGAAGTTCCAAACTTGGAGTATGATATTCACGCTCACAATGACTTAGGTCTAGCAGTAGCAAATGCATTGGCTGCAG
TAGAAGGTGGAGCTACGATTGTTCATGCAACGGTTAATGGGCTTGGAGAGAGGGTTGGTATAGTACCTTTGCAACAAATC
GTAGCAGCTATTAAGTATCATTTTGGTATAGAAGTAGTTAAACTAGATAAATTACAGTACGTTTCCAGTTTAATTGAAAA
GTACAGTGGAATTCCGATGCCACCTAATTATCCCATAACTGGGGATTACGCTTTTTTGCATAAGGCAGGAGTTCATGTTG
CGGGTGTGTTGAGTGATCCTAGAACATATGAATTTATGCCTCCAGAGACGTTTGGTAGAACAAGAGATTACACTATTGAT
AAATATACAGGAAAGCATGCGTTAAGAGATAAATATGAAAAACTAGGTGTGAAAATCAGTGAGGCTGAAATGGATCAGAT
TTTAGCTAAAATTAAGTCAAATACGACTATAAGATTTTACAGAGATGTGGATTTACTAGAGTTAGCTGAAGAAGTTACCG
GAAGAGTTTTGAAGCCAAGACCACCTGAGCAAATAGAAGCGTTAATTTCAGTTAAGTGTGATTCTAACGTTTATACCACA
TCAGTAACTCGTCGTTTATCAGTTATTAATGGCGTTAAAGAGGTTATGGAAATTTCAGGAGATTATGACATACTGGTCAA
GGTTCAAGCTAAGGACTCTAATGAATTAAACCAGATAATCGAAAGTATAAGAGCAACTAAAGGTGTGAGATCAACATTAA
CATCATTAGTCCTTAAGAAAATGTAA

Upstream 100 bases:

>100_bases
GAATGTGAATCAGTTGATACTAACTACTATAAAATAGTTCGTATGTCGAACCTTTCTTTACCGAATTCATTATATATCCT
TTACCTTAATTCTCACTTGT

Downstream 100 bases:

>100_bases
ATTCCCTTCTAAAACTTTTTAAGGAAAACGAGTTTATATTTAAAATGTGCGGGGGTGCCCGAGCTGGTCAAAGGGGGCGG
ACTCAAGACCCCTTAAGTGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRIVKLKKEGIITSEIVGHSRAV
KRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRVIAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDA
GADRVSIADTVGILYPSKTKELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQI
VAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDPRTYEFMPPETFGRTRDYTID
KYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFYRDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTT
SVTRRLSVINGVKEVMEISGDYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM

Sequences:

>Translated_461_residues
MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRIVKLKKEGIITSEIVGHSRAV
KRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRVIAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDA
GADRVSIADTVGILYPSKTKELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQI
VAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDPRTYEFMPPETFGRTRDYTID
KYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFYRDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTT
SVTRRLSVINGVKEVMEISGDYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM
>Mature_461_residues
MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRIVKLKKEGIITSEIVGHSRAV
KRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRVIAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDA
GADRVSIADTVGILYPSKTKELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQI
VAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDPRTYEFMPPETFGRTRDYTID
KYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFYRDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTT
SVTRRLSVINGVKEVMEISGDYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=243, Percent_Identity=23.8683127572016, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI109150422, Length=241, Percent_Identity=24.0663900414938, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI109150427, Length=241, Percent_Identity=24.0663900414938, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1786261, Length=357, Percent_Identity=33.8935574229692, Blast_Score=204, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6320071, Length=384, Percent_Identity=34.375, Blast_Score=216, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6320019, Length=398, Percent_Identity=33.4170854271357, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6324682, Length=307, Percent_Identity=24.1042345276873, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582381, Length=242, Percent_Identity=22.7272727272727, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_SULSO (Q97ZE0)

Other databases:

- EMBL:   AE006641
- PIR:   E90249
- RefSeq:   NP_342462.1
- ProteinModelPortal:   Q97ZE0
- GeneID:   1455219
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0977
- NMPDR:   fig|273057.1.peg.885
- HOGENOM:   HBG701974
- OMA:   FTAEDAS
- PhylomeDB:   Q97ZE0
- ProtClustDB:   PRK00915
- BioCyc:   SSOL273057:SSO0977-MONOMER
- BRENDA:   2.3.3.13
- GO:   GO:0005622
- HAMAP:   MF_01025_A
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02146

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like; SSF54909 Dimer_A_B_barrel

EC number: =2.3.3.13

Molecular weight: Translated: 50882; Mature: 50882

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRI
CEEEEEEHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHH
VKLKKEGIITSEIVGHSRAVKRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRV
HHHHHCCCCHHHHHCCHHHHHHHHHHHEEECCCEEEEEECCCCEEEEHHHHHHHHHHHHH
IAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDAGADRVSIADTVGILYPSKTK
HHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEHHHHHCCCCCEEHHHHHEEECCCHHH
ELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCHHHHH
VAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDP
HHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCC
RTYEFMPPETFGRTRDYTIDKYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFY
CCEECCCCHHCCCCCCCEEECCCCCHHHHHHHHHHCCEECHHHHHHHHHHHHCCCEEEEE
RDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTTSVTRRLSVINGVKEVMEISG
ECCHHHHHHHHHHCCCCCCCCHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCC
DYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRI
CEEEEEEHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHH
VKLKKEGIITSEIVGHSRAVKRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRV
HHHHHCCCCHHHHHCCHHHHHHHHHHHEEECCCEEEEEECCCCEEEEHHHHHHHHHHHHH
IAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDAGADRVSIADTVGILYPSKTK
HHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEHHHHHCCCCCEEHHHHHEEECCCHHH
ELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQI
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCHHHHH
VAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDP
HHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCC
RTYEFMPPETFGRTRDYTIDKYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFY
CCEECCCCHHCCCCCCCEEECCCCCHHHHHHHHHHCCEECHHHHHHHHHHHHCCCEEEEE
RDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTTSVTRRLSVINGVKEVMEISG
ECCHHHHHHHHHHCCCCCCCCHHHHHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCC
DYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726