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The map label for this gene is topA [H]

Identifier: 15897795

GI number: 15897795

Start: 772004

End: 774010

Strand: Direct

Name: topA [H]

Synonym: SSO0907

Alternate gene names: 15897795

Gene position: 772004-774010 (Clockwise)

Preceding gene: 15897794

Following gene: 15897799

Centisome position: 25.8

GC content: 32.24

Gene sequence:

>2007_bases
ATGAATTTATGTAATGTAAACAACTATTACCTTATAATTGCAGAAAAATCAAAAGCTGCTAAAAAAATCGCAGAAGCTCT
TTCAGAGAAGCCTATTTTGTGTAGAAAATATAACGTTAGTTATTGGATAATAAAAGATCACAATAGCAGCAAATATGTTA
TAGTTCCTGCAGCAGGACATCTTTTTGGGTTAAAAGGCGAGAGCGGTTTTCCGGTATATGATGCGGACTGGAAACCTCTA
TGGGAAATTGATAAGAATAGTTACTATACAAAAAGGTACTATCAACTTATCTCATCTCTTAGCAAGTATGCTTTAGGTTT
TATCAATGCTTGTGATTACGATATAGAAGGTTCTGTAATCGGCTATTTAATAATCAAAAATCTAGGCGATATTAAGAAGG
CTAAACGAATGAAATTTTCAGCACTGACTAAAAGTGATATATTATCTGCATTTAGAAACATTTCTGCATTAGATTACGAT
ATGATAAACGCTGGAATAGCCAGACATAAAATTGACTGGCTATGGGGAATTAACGTTAGTAGGGCTCTTATGATCTCTCT
ACAAGATTTCGCAAAGAAAAGAGTGATATTAAGTGCGGGTAGGGTTCAAAGTCCAACTCTAGTTCAAGTTGTCAACTCGG
AAATTGAAAGAAACCTATTTATTCCCCTACCTAAATTTACCGTTTCAATTATCGTGAAAATTAAAGATTATTCATTAAAC
ATTAAGGTAAATAAGGAATTCGAAAAAATTACCGAAGCAAAGGAATTCTTAAACAAACTAATAAATAAAACAGTAAAAGT
TGTTGAAGTTGAAAATAGGGTTAGGTTATTAGAAAGACCCTCTCCATTTAATCTTACTGATCTCCAAATAGAAGCTGGCA
GAATATATGGTATATCCCCATATAACGTAGAACGTATAGCAGAAGATCTTTATTTGGACGGTCTAATAAGTTACCCAAGA
ACTAACAGTCAAAAAATTCCATCAACTATCAGCATTTATAATATAATCAAAGGCTTAGAGAACAGTTCGTATAGGAAACT
AGTTGATTTAGTAAGGAAAATCACTGGGGGGAAATATGTAGTTAAGCAGGGCATTAAGGATGATCCTGCACACCCCGCAA
TCCACCCTACTGGTGAAGCTCCCAAAAACTTACCTAATAGCAAATTCAAGATATATGACTTAATAGCAAGAAGATTTTTG
GGGTCAGTATCTGCTGATGCTAAATTATCTAATACTATTTACACCTTGAAAGTTAGTGATTTCCCATTAGAGTTTACGGT
CTCATATACAAAAATACTAGAAAGAAATTGGCTAGATATATATCACTTTCATAATGTAAAAGAAGATAAACCAATATTTC
TCTCAAAAGGTGATGAGGGTAAAATAGTAGATGGAAAAGTAAATATTAGTTTAAGCAAGCCCACTTCTAGGTATACAAAG
GTTTCATTACTCAAATGGATGGAGTCTTCTAATTTGGGTACAGAAGCCACTAGAGGAAGAATAATCGAGATCTTAGTAAA
GAGAAAATATTTAACTAACAATGGGCGATACATAATTCCTACAAAATTGGGATTCTATATTGCTGAAATATTAAACAAAT
TTTTCCCAGATATAGTTGATGTTAGGATGACTGCAGATATGGAAAGTAAATTAGAAATGATAAAGACTGGCAAAGTTTTG
GAAAGCAAAGTGATTAAAGAAAACATAGAAAAATTAAATAAATTCATAGAGGAATACAAGGTAAATAAGGATAAAGTAGG
AGAGTCATTAGCTAAAGCGTTAGGTCTTATAAAAATTGTTAAGTGTAAGTATTGTGATCTGGAGCAGTATAAGGATGGAT
TATGTAAATATCACTATGAAGCCAAAGTAAGGCTTTTAGATGCTGTGGAAATTTGGAAAGAAAGGACAAAATATGATCAT
AAAAAGATTTTAAAGAGAATTAGTAGTAGTAAATCAACGGGTAAATACGTAAAAGATATAGTAACTTATATGCTAAGCAG
TGAATGA

Upstream 100 bases:

>100_bases
CTGAGGAGGAGTTTAGGAGAGGAGTTCTTCCAATAACTATTAGAAGAAGATTACCAAATGGAAAAATAATTTTATTATCT
CTTAGAAAGAGTTAAATAAG

Downstream 100 bases:

>100_bases
TGAAAGTCATGCCAATCCGAAAAGATGAGGTAAACTCTGGGGTAACTGAGTTTCTATAATGCCTTAAATTTCTCTGTCCA
AGCCTCATAAGCTTTTATTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MNLCNVNNYYLIIAEKSKAAKKIAEALSEKPILCRKYNVSYWIIKDHNSSKYVIVPAAGHLFGLKGESGFPVYDADWKPL
WEIDKNSYYTKRYYQLISSLSKYALGFINACDYDIEGSVIGYLIIKNLGDIKKAKRMKFSALTKSDILSAFRNISALDYD
MINAGIARHKIDWLWGINVSRALMISLQDFAKKRVILSAGRVQSPTLVQVVNSEIERNLFIPLPKFTVSIIVKIKDYSLN
IKVNKEFEKITEAKEFLNKLINKTVKVVEVENRVRLLERPSPFNLTDLQIEAGRIYGISPYNVERIAEDLYLDGLISYPR
TNSQKIPSTISIYNIIKGLENSSYRKLVDLVRKITGGKYVVKQGIKDDPAHPAIHPTGEAPKNLPNSKFKIYDLIARRFL
GSVSADAKLSNTIYTLKVSDFPLEFTVSYTKILERNWLDIYHFHNVKEDKPIFLSKGDEGKIVDGKVNISLSKPTSRYTK
VSLLKWMESSNLGTEATRGRIIEILVKRKYLTNNGRYIIPTKLGFYIAEILNKFFPDIVDVRMTADMESKLEMIKTGKVL
ESKVIKENIEKLNKFIEEYKVNKDKVGESLAKALGLIKIVKCKYCDLEQYKDGLCKYHYEAKVRLLDAVEIWKERTKYDH
KKILKRISSSKSTGKYVKDIVTYMLSSE

Sequences:

>Translated_668_residues
MNLCNVNNYYLIIAEKSKAAKKIAEALSEKPILCRKYNVSYWIIKDHNSSKYVIVPAAGHLFGLKGESGFPVYDADWKPL
WEIDKNSYYTKRYYQLISSLSKYALGFINACDYDIEGSVIGYLIIKNLGDIKKAKRMKFSALTKSDILSAFRNISALDYD
MINAGIARHKIDWLWGINVSRALMISLQDFAKKRVILSAGRVQSPTLVQVVNSEIERNLFIPLPKFTVSIIVKIKDYSLN
IKVNKEFEKITEAKEFLNKLINKTVKVVEVENRVRLLERPSPFNLTDLQIEAGRIYGISPYNVERIAEDLYLDGLISYPR
TNSQKIPSTISIYNIIKGLENSSYRKLVDLVRKITGGKYVVKQGIKDDPAHPAIHPTGEAPKNLPNSKFKIYDLIARRFL
GSVSADAKLSNTIYTLKVSDFPLEFTVSYTKILERNWLDIYHFHNVKEDKPIFLSKGDEGKIVDGKVNISLSKPTSRYTK
VSLLKWMESSNLGTEATRGRIIEILVKRKYLTNNGRYIIPTKLGFYIAEILNKFFPDIVDVRMTADMESKLEMIKTGKVL
ESKVIKENIEKLNKFIEEYKVNKDKVGESLAKALGLIKIVKCKYCDLEQYKDGLCKYHYEAKVRLLDAVEIWKERTKYDH
KKILKRISSSKSTGKYVKDIVTYMLSSE
>Mature_668_residues
MNLCNVNNYYLIIAEKSKAAKKIAEALSEKPILCRKYNVSYWIIKDHNSSKYVIVPAAGHLFGLKGESGFPVYDADWKPL
WEIDKNSYYTKRYYQLISSLSKYALGFINACDYDIEGSVIGYLIIKNLGDIKKAKRMKFSALTKSDILSAFRNISALDYD
MINAGIARHKIDWLWGINVSRALMISLQDFAKKRVILSAGRVQSPTLVQVVNSEIERNLFIPLPKFTVSIIVKIKDYSLN
IKVNKEFEKITEAKEFLNKLINKTVKVVEVENRVRLLERPSPFNLTDLQIEAGRIYGISPYNVERIAEDLYLDGLISYPR
TNSQKIPSTISIYNIIKGLENSSYRKLVDLVRKITGGKYVVKQGIKDDPAHPAIHPTGEAPKNLPNSKFKIYDLIARRFL
GSVSADAKLSNTIYTLKVSDFPLEFTVSYTKILERNWLDIYHFHNVKEDKPIFLSKGDEGKIVDGKVNISLSKPTSRYTK
VSLLKWMESSNLGTEATRGRIIEILVKRKYLTNNGRYIIPTKLGFYIAEILNKFFPDIVDVRMTADMESKLEMIKTGKVL
ESKVIKENIEKLNKFIEEYKVNKDKVGESLAKALGLIKIVKCKYCDLEQYKDGLCKYHYEAKVRLLDAVEIWKERTKYDH
KKILKRISSSKSTGKYVKDIVTYMLSSE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic type I/III topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=614, Percent_Identity=25.8957654723127, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4507635, Length=479, Percent_Identity=24.4258872651357, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1788061, Length=637, Percent_Identity=26.8445839874411, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787529, Length=463, Percent_Identity=26.9978401727862, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=673, Percent_Identity=24.665676077266, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=25.7367387033399, Blast_Score=119, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=655, Percent_Identity=25.4961832061069, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585251, Length=638, Percent_Identity=24.7648902821317, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640096, Length=593, Percent_Identity=24.4519392917369, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 76574; Mature: 76574

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLCNVNNYYLIIAEKSKAAKKIAEALSEKPILCRKYNVSYWIIKDHNSSKYVIVPAAGH
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCEEEEEECCCC
LFGLKGESGFPVYDADWKPLWEIDKNSYYTKRYYQLISSLSKYALGFINACDYDIEGSVI
EEEECCCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GYLIIKNLGDIKKAKRMKFSALTKSDILSAFRNISALDYDMINAGIARHKIDWLWGINVS
EHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCEEEEECCCCC
RALMISLQDFAKKRVILSAGRVQSPTLVQVVNSEIERNLFIPLPKFTVSIIVKIKDYSLN
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCEEEECCCEEEEEEEEEEEEEEE
IKVNKEFEKITEAKEFLNKLINKTVKVVEVENRVRLLERPSPFNLTDLQIEAGRIYGISP
EEECCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCEEEEEEECCEEEECCC
YNVERIAEDLYLDGLISYPRTNSQKIPSTISIYNIIKGLENSSYRKLVDLVRKITGGKYV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEE
VKQGIKDDPAHPAIHPTGEAPKNLPNSKFKIYDLIARRFLGSVSADAKLSNTIYTLKVSD
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCEEEEEEECC
FPLEFTVSYTKILERNWLDIYHFHNVKEDKPIFLSKGDEGKIVDGKVNISLSKPTSRYTK
CCEEEEEHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCEEECEEEEEECCCCCHHHH
VSLLKWMESSNLGTEATRGRIIEILVKRKYLTNNGRYIIPTKLGFYIAEILNKFFPDIVD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCHHEE
VRMTADMESKLEMIKTGKVLESKVIKENIEKLNKFIEEYKVNKDKVGESLAKALGLIKIV
EEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEE
KCKYCDLEQYKDGLCKYHYEAKVRLLDAVEIWKERTKYDHKKILKRISSSKSTGKYVKDI
HCCCCCHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHH
VTYMLSSE
HHHHHCCC
>Mature Secondary Structure
MNLCNVNNYYLIIAEKSKAAKKIAEALSEKPILCRKYNVSYWIIKDHNSSKYVIVPAAGH
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCEEEEEECCCC
LFGLKGESGFPVYDADWKPLWEIDKNSYYTKRYYQLISSLSKYALGFINACDYDIEGSVI
EEEECCCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
GYLIIKNLGDIKKAKRMKFSALTKSDILSAFRNISALDYDMINAGIARHKIDWLWGINVS
EHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCEEEEECCCCC
RALMISLQDFAKKRVILSAGRVQSPTLVQVVNSEIERNLFIPLPKFTVSIIVKIKDYSLN
HHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCEEEECCCEEEEEEEEEEEEEEE
IKVNKEFEKITEAKEFLNKLINKTVKVVEVENRVRLLERPSPFNLTDLQIEAGRIYGISP
EEECCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCCCEEEEEEECCEEEECCC
YNVERIAEDLYLDGLISYPRTNSQKIPSTISIYNIIKGLENSSYRKLVDLVRKITGGKYV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEE
VKQGIKDDPAHPAIHPTGEAPKNLPNSKFKIYDLIARRFLGSVSADAKLSNTIYTLKVSD
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCEEEEEEECC
FPLEFTVSYTKILERNWLDIYHFHNVKEDKPIFLSKGDEGKIVDGKVNISLSKPTSRYTK
CCEEEEEHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCEEECEEEEEECCCCCHHHH
VSLLKWMESSNLGTEATRGRIIEILVKRKYLTNNGRYIIPTKLGFYIAEILNKFFPDIVD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCHHEE
VRMTADMESKLEMIKTGKVLESKVIKENIEKLNKFIEEYKVNKDKVGESLAKALGLIKIV
EEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEE
KCKYCDLEQYKDGLCKYHYEAKVRLLDAVEIWKERTKYDHKKILKRISSSKSTGKYVKDI
HCCCCCHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHH
VTYMLSSE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]