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The map label for this gene is ppsA-1 [H]

Identifier: 15897773

GI number: 15897773

Start: 751957

End: 754356

Strand: Direct

Name: ppsA-1 [H]

Synonym: SSO0883

Alternate gene names: 15897773

Gene position: 751957-754356 (Clockwise)

Preceding gene: 15897772

Following gene: 15897775

Centisome position: 25.13

GC content: 37.38

Gene sequence:

>2400_bases
TTGGGGAGTTTCCTTTTGGTTGAGGCAATTAGTAGTGAAGAAGATCTTATTCTCGACGTAAGTCAAGTCACTAAGGATAT
GGTACAATTAGCTGGAGGCAAAGGTGCAAACCTTGGGGAGTTAACCAGCATTGGAGTTAGAGTACCTCCAGCCTTTATAC
TTACTTCTAAAGCTTTTAAATATTTTCTTGAATACAACAATCTTTTTGACAAAATTAGAGACACTTTAAGTAGTTCTGAA
ACCTCAGAGGAAGCTAGTGAAAAAATAAAACAGTTGATAAAGAATGCTAAGATGCCAGATAAATTAAGTAGCATGATTTA
TCAAGCTTATGATGAATTAAGTAAAAAGGTTGGAAAGGAAATCTTAGTAGCTGTAAGGTCTTCGGCTACTGCAGAGGATA
TTGAGACAGCAAGTTTCGCTGGACAGCAAGATACTTATCTTAATGTTACTAAGGATGAACTTATTGACAGGATTAAGGAT
GTATGGGCTAGTCTCTATAACGCAAGGGCTATGGAATATAGAAAGAGTAAAGGTATAGACGACTTATCAATACTTATAGC
AGTAGTTGTTCAAAAAATGGTTAATTCGAGATCTGCTGGAGTAATGTTTACTCTCCATCCAGTTACTGGAGATGAAAAGT
ATATCATGATAGAATCTAATTGGGGTTTAGGAGAAAGTGTAGTCGGTGGTAAGGTTACCCCAGATGAGGTCTTAATAGAA
AAATCTACCTTAAGAATTGTGGAAAAAAAGGTTTCTAATAAGAATATTAAAATCGTATATGATAAACAACTTAAGAAGAA
TGTTACTATTACTTTAGATGAGAAGGAATCCAGATTAATGAGCATAACAGACGAAGAGGCAATAGAACTAGCAAAATTAG
CACTTAAGATCGAAGAACATTATAAGAGGCCAATGGACATTGAGTGGGCTATCGATAATGATCTAAGTTTCCCTGAGAAT
ATCTTCATAGTTCAAGCTAGGCCAGAAACTTTCTGGTCTTCTAAAAGGAAAGAAAATAAGAACATTGCGGAAAAGAGTGC
TCCTATAGGTGGTAAAGTACTAGTCAGAGGGCTTGCAGCTTCTCCAGGTATCGCCTTTGGTAAGGCAAAAATCATACTTG
ACATTAAAGATCCTAAAGTTCATGAGTTTCAGAAGGGTGAAATCCTAGTCACGAAAATGACAGATCCAGATTGGGTACCA
TTAATGAAGATAGCTGGGGCAATAATAACAGATGAGGGAGGAATGACAAGTCACGCGGCTATTGTATCCAGAGAATTGGG
AATTCCAGCTATAGTTGGAAGTAGAGAAGCGACTAAGATAATACGAGATAATCAAGAGATCACTGTAGATGCAATTAGGG
GTATTGTGTATGAGGGTAAGGTATTACAAACAAGTGAGACTGTATCTCAACAAGCTCAACCATCTATCGGAATTCAAGGA
ATAAGTAGGGAAGTTCTATTAAGTCTTTATCCGGTTACTGCAACTAAAATTTACATGAATTTAGGTGAACCCGATGTTAT
AGATAAATATTTGGATCTTCCCTTTGACGGAATAGGGCTCATGAGAATTGAATTTATTGTAAGCGAATGGGTTAGATATC
ATCCGCTTTACCTAATAAAAATAGGCAATGCTGAATTATTTGTGGATAAGTTAGCGGAGGGAATAGCTAAAGTAGCAAGT
GCAATATACCCTAGACCAGTTGTTGTTAGATTTTCAGATTTCAAAACTAACGAATATAAGAAATTGATTGGTGGAGAGGA
GTTTGAGCCAGATGAGAGGAATCCAATGATAGGTTGGAGAGGGGTTTCCAGATATGTAAGTAAAGAGTATGAGCCTGCAT
TTAGGCTAGAGGCTAAAGCTATACGCAAAGTTAGGGAAGAGATGGGACTTAAGAACGTATGGGTAATGTTTCCCTTTGTC
AGAACTACATGGGAGCTAGAGAAGGCAATAAAAATAATGGAGGAGGAAGGTTTAAGAAGAGATTCTGATTTCAAGGTCTG
GATAATGGCTGAAGTTCCCTCAGTAGTTGTTCTTGCGGAAGAGTTTGCGAAGATAGTTGATGGATTCAGTGTAGGTAGTA
ACGATTTAGCACAATTAACTTTAGGTGTTGACAGGGATTCCGAGTTACTAGCGAGGATGGGATATTATGATGAGAGGGAT
CCAGCTGTTTTAGAATCCATAAGAAAGCTAATCAAAGCTGCCCATAAGTATGGCAAAACAGTCTCAATATGTGGACAAGC
ACCTAGTGTTTATCCAGCAGTTGTTGAATATTTAGTCAAAGCTGGAATAGATAGCATTAGTGTAAATCCAGATGCGGTTA
TTAATGTTAGGAGGCAAGTAGCTTCGATCGAGCAACAAATTATACTTAGAAACCTTAATGGAAAAAGGAAGAACAAGTAG

Upstream 100 bases:

>100_bases
AAGTATCTAACCAAGAACAGAAATAATATCTGATTCATAGATTTTTTCCTAAGATTTATACGGTAAAATAGTTTTTTAAG
GTTTGGATTGATAATATGTA

Downstream 100 bases:

>100_bases
ACAATTTTTTAATTATTTTTTATATTATAACAATTTTTGCATATTCTTCTAGCACCGTCGAAATACGCTGTGCATTCTTC
ACAAATTGTATTTCCGCAGA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MGSFLLVEAISSEEDLILDVSQVTKDMVQLAGGKGANLGELTSIGVRVPPAFILTSKAFKYFLEYNNLFDKIRDTLSSSE
TSEEASEKIKQLIKNAKMPDKLSSMIYQAYDELSKKVGKEILVAVRSSATAEDIETASFAGQQDTYLNVTKDELIDRIKD
VWASLYNARAMEYRKSKGIDDLSILIAVVVQKMVNSRSAGVMFTLHPVTGDEKYIMIESNWGLGESVVGGKVTPDEVLIE
KSTLRIVEKKVSNKNIKIVYDKQLKKNVTITLDEKESRLMSITDEEAIELAKLALKIEEHYKRPMDIEWAIDNDLSFPEN
IFIVQARPETFWSSKRKENKNIAEKSAPIGGKVLVRGLAASPGIAFGKAKIILDIKDPKVHEFQKGEILVTKMTDPDWVP
LMKIAGAIITDEGGMTSHAAIVSRELGIPAIVGSREATKIIRDNQEITVDAIRGIVYEGKVLQTSETVSQQAQPSIGIQG
ISREVLLSLYPVTATKIYMNLGEPDVIDKYLDLPFDGIGLMRIEFIVSEWVRYHPLYLIKIGNAELFVDKLAEGIAKVAS
AIYPRPVVVRFSDFKTNEYKKLIGGEEFEPDERNPMIGWRGVSRYVSKEYEPAFRLEAKAIRKVREEMGLKNVWVMFPFV
RTTWELEKAIKIMEEEGLRRDSDFKVWIMAEVPSVVVLAEEFAKIVDGFSVGSNDLAQLTLGVDRDSELLARMGYYDERD
PAVLESIRKLIKAAHKYGKTVSICGQAPSVYPAVVEYLVKAGIDSISVNPDAVINVRRQVASIEQQIILRNLNGKRKNK

Sequences:

>Translated_799_residues
MGSFLLVEAISSEEDLILDVSQVTKDMVQLAGGKGANLGELTSIGVRVPPAFILTSKAFKYFLEYNNLFDKIRDTLSSSE
TSEEASEKIKQLIKNAKMPDKLSSMIYQAYDELSKKVGKEILVAVRSSATAEDIETASFAGQQDTYLNVTKDELIDRIKD
VWASLYNARAMEYRKSKGIDDLSILIAVVVQKMVNSRSAGVMFTLHPVTGDEKYIMIESNWGLGESVVGGKVTPDEVLIE
KSTLRIVEKKVSNKNIKIVYDKQLKKNVTITLDEKESRLMSITDEEAIELAKLALKIEEHYKRPMDIEWAIDNDLSFPEN
IFIVQARPETFWSSKRKENKNIAEKSAPIGGKVLVRGLAASPGIAFGKAKIILDIKDPKVHEFQKGEILVTKMTDPDWVP
LMKIAGAIITDEGGMTSHAAIVSRELGIPAIVGSREATKIIRDNQEITVDAIRGIVYEGKVLQTSETVSQQAQPSIGIQG
ISREVLLSLYPVTATKIYMNLGEPDVIDKYLDLPFDGIGLMRIEFIVSEWVRYHPLYLIKIGNAELFVDKLAEGIAKVAS
AIYPRPVVVRFSDFKTNEYKKLIGGEEFEPDERNPMIGWRGVSRYVSKEYEPAFRLEAKAIRKVREEMGLKNVWVMFPFV
RTTWELEKAIKIMEEEGLRRDSDFKVWIMAEVPSVVVLAEEFAKIVDGFSVGSNDLAQLTLGVDRDSELLARMGYYDERD
PAVLESIRKLIKAAHKYGKTVSICGQAPSVYPAVVEYLVKAGIDSISVNPDAVINVRRQVASIEQQIILRNLNGKRKNK
>Mature_798_residues
GSFLLVEAISSEEDLILDVSQVTKDMVQLAGGKGANLGELTSIGVRVPPAFILTSKAFKYFLEYNNLFDKIRDTLSSSET
SEEASEKIKQLIKNAKMPDKLSSMIYQAYDELSKKVGKEILVAVRSSATAEDIETASFAGQQDTYLNVTKDELIDRIKDV
WASLYNARAMEYRKSKGIDDLSILIAVVVQKMVNSRSAGVMFTLHPVTGDEKYIMIESNWGLGESVVGGKVTPDEVLIEK
STLRIVEKKVSNKNIKIVYDKQLKKNVTITLDEKESRLMSITDEEAIELAKLALKIEEHYKRPMDIEWAIDNDLSFPENI
FIVQARPETFWSSKRKENKNIAEKSAPIGGKVLVRGLAASPGIAFGKAKIILDIKDPKVHEFQKGEILVTKMTDPDWVPL
MKIAGAIITDEGGMTSHAAIVSRELGIPAIVGSREATKIIRDNQEITVDAIRGIVYEGKVLQTSETVSQQAQPSIGIQGI
SREVLLSLYPVTATKIYMNLGEPDVIDKYLDLPFDGIGLMRIEFIVSEWVRYHPLYLIKIGNAELFVDKLAEGIAKVASA
IYPRPVVVRFSDFKTNEYKKLIGGEEFEPDERNPMIGWRGVSRYVSKEYEPAFRLEAKAIRKVREEMGLKNVWVMFPFVR
TTWELEKAIKIMEEEGLRRDSDFKVWIMAEVPSVVVLAEEFAKIVDGFSVGSNDLAQLTLGVDRDSELLARMGYYDERDP
AVLESIRKLIKAAHKYGKTVSICGQAPSVYPAVVEYLVKAGIDSISVNPDAVINVRRQVASIEQQIILRNLNGKRKNK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=813, Percent_Identity=39.6063960639606, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1788756, Length=452, Percent_Identity=27.8761061946903, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI48994992, Length=359, Percent_Identity=27.0194986072423, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789193, Length=398, Percent_Identity=27.1356783919598, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788726, Length=206, Percent_Identity=31.5533980582524, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17564524, Length=309, Percent_Identity=30.7443365695793, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 89394; Mature: 89263

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFLLVEAISSEEDLILDVSQVTKDMVQLAGGKGANLGELTSIGVRVPPAFILTSKAFK
CCCEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
YFLEYNNLFDKIRDTLSSSETSEEASEKIKQLIKNAKMPDKLSSMIYQAYDELSKKVGKE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILVAVRSSATAEDIETASFAGQQDTYLNVTKDELIDRIKDVWASLYNARAMEYRKSKGID
HHHHHHCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLSILIAVVVQKMVNSRSAGVMFTLHPVTGDEKYIMIESNWGLGESVVGGKVTPDEVLIE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHH
KSTLRIVEKKVSNKNIKIVYDKQLKKNVTITLDEKESRLMSITDEEAIELAKLALKIEEH
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHEECCHHHHHHHHHHHHHHHHH
YKRPMDIEWAIDNDLSFPENIFIVQARPETFWSSKRKENKNIAEKSAPIGGKVLVRGLAA
CCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCC
SPGIAFGKAKIILDIKDPKVHEFQKGEILVTKMTDPDWVPLMKIAGAIITDEGGMTSHAA
CCCCEECCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCCHHHH
IVSRELGIPAIVGSREATKIIRDNQEITVDAIRGIVYEGKVLQTSETVSQQAQPSIGIQG
HHHHCCCCCEEECCHHHHHHHHCCCCEEHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCC
ISREVLLSLYPVTATKIYMNLGEPDVIDKYLDLPFDGIGLMRIEFIVSEWVRYHPLYLIK
CCHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
IGNAELFVDKLAEGIAKVASAIYPRPVVVRFSDFKTNEYKKLIGGEEFEPDERNPMIGWR
ECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHH
GVSRYVSKEYEPAFRLEAKAIRKVREEMGLKNVWVMFPFVRTTWELEKAIKIMEEEGLRR
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCC
DSDFKVWIMAEVPSVVVLAEEFAKIVDGFSVGSNDLAQLTLGVDRDSELLARMGYYDERD
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCC
PAVLESIRKLIKAAHKYGKTVSICGQAPSVYPAVVEYLVKAGIDSISVNPDAVINVRRQV
HHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ASIEQQIILRNLNGKRKNK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GSFLLVEAISSEEDLILDVSQVTKDMVQLAGGKGANLGELTSIGVRVPPAFILTSKAFK
CCEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
YFLEYNNLFDKIRDTLSSSETSEEASEKIKQLIKNAKMPDKLSSMIYQAYDELSKKVGKE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILVAVRSSATAEDIETASFAGQQDTYLNVTKDELIDRIKDVWASLYNARAMEYRKSKGID
HHHHHHCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLSILIAVVVQKMVNSRSAGVMFTLHPVTGDEKYIMIESNWGLGESVVGGKVTPDEVLIE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHH
KSTLRIVEKKVSNKNIKIVYDKQLKKNVTITLDEKESRLMSITDEEAIELAKLALKIEEH
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCHHEECCHHHHHHHHHHHHHHHHH
YKRPMDIEWAIDNDLSFPENIFIVQARPETFWSSKRKENKNIAEKSAPIGGKVLVRGLAA
CCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHCC
SPGIAFGKAKIILDIKDPKVHEFQKGEILVTKMTDPDWVPLMKIAGAIITDEGGMTSHAA
CCCCEECCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCCHHHH
IVSRELGIPAIVGSREATKIIRDNQEITVDAIRGIVYEGKVLQTSETVSQQAQPSIGIQG
HHHHCCCCCEEECCHHHHHHHHCCCCEEHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCC
ISREVLLSLYPVTATKIYMNLGEPDVIDKYLDLPFDGIGLMRIEFIVSEWVRYHPLYLIK
CCHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
IGNAELFVDKLAEGIAKVASAIYPRPVVVRFSDFKTNEYKKLIGGEEFEPDERNPMIGWR
ECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHH
GVSRYVSKEYEPAFRLEAKAIRKVREEMGLKNVWVMFPFVRTTWELEKAIKIMEEEGLRR
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCC
DSDFKVWIMAEVPSVVVLAEEFAKIVDGFSVGSNDLAQLTLGVDRDSELLARMGYYDERD
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCC
PAVLESIRKLIKAAHKYGKTVSICGQAPSVYPAVVEYLVKAGIDSISVNPDAVINVRRQV
HHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ASIEQQIILRNLNGKRKNK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]