Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 15897626

GI number: 15897626

Start: 616781

End: 617791

Strand: Reverse

Name: leuB

Synonym: SSO0723

Alternate gene names: 15897626

Gene position: 617791-616781 (Counterclockwise)

Preceding gene: 15897627

Following gene: 15897625

Centisome position: 20.65

GC content: 36.7

Gene sequence:

>1011_bases
ATGACTTTTCGAGTAGCTGTAATTCCTGGTGATGGAATAGGCCCTGAGTTATATGAGGGAAGTAAAAGAATTATAGCTAA
GTTAATAGAGAAATATAATCTTGATATAGACTTAATTGAAGTTGAAGCTGGAGATGTCGCATTAAATAAATATGGTGAAG
CCTTACCTAGACACACCTTAGACGTTATAGAAAAGGCGGATATGATTCTAAAAGGTCCGGTTGGAGAGACCGCAATGGAT
GTTGTAGTTAAATTGAGGCAAATGTATGATATGTACGCTAATATTAGACCGGCTAAATCACTGCCTAATGTTCAGTCGAA
ATATCCGAACGTTGATTTAGTAATCGTCAGGGAAAATACAGAAGATCTATATAAGGGGTTTGAATTCGTTACATCTACTG
GAGTCACAATAGCTATTAAAGTAACTACTGAGTTTGCCTCTAAAAGAATAGTTAACGTGGCATTAAATTATGCTTTAATG
AGACGTCGTAAAGTTACATGCGTCCATAAGGCTAATGTTATGAGAGTTACAGACGGACTTTTCGCTAGTGTTTGCAGAGA
AATTTTGAAAGGTAAGGTGAATTTTGAGGAGATGTATGTTGATGCAGCTGCAGCTAATTTAGTACGAGATCCAACTCGGT
TTGATGTAATAGTAACTAGTAACGTTTATGGTGATATTTTAAGCGATGAGGCCAGCCAAATAGCGGGAAGTTTAGGTCTA
GCACCATCTGCAAATATTGGAGATAGGAAATCTATGTTCGAGCCAGTTCATGGAGCTGCTTTTGATATTGCGGGAAAAGG
GATAGCTAATCCTACGGCATTTCTATTATCTGTTTCCATGATGCTTAGTAGAATGTATGAGTTATCTAAGGAGAATAAAT
ACATTCTGGCATCAAAGTCTTTAGAGAATGCGATAATAGAGACTTACAAAAGTGGGAATAAGTTAACGGAGGATGTTGGT
GGTAGTGCTAAGTTAAAGGATATGGTGGATGAAATTTATAAATATATGTAA

Upstream 100 bases:

>100_bases
TGTTATAAAGCGTACATCTCAATATACTTCTAGTCTCTTAGAGGAGATATTAAATAAGATGAGATCAAAAGGTTTTAAGC
TAGACTAGTTTTCTTTACAT

Downstream 100 bases:

>100_bases
CGTGAACGTATATAATGTAAAGTTTAATTGACAACGAAGTTTAATTTTTATAAACTTGTCAAGTTTAATAGGGTATGGTG
AGTTGAATTATCTGCCGTTA

Product: 3-isopropylmalate dehydrogenase (leuB)

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDVIEKADMILKGPVGETAMD
VVVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALM
RRRKVTCVHKANVMRVTDGLFASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVG
GSAKLKDMVDEIYKYM

Sequences:

>Translated_336_residues
MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDVIEKADMILKGPVGETAMD
VVVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALM
RRRKVTCVHKANVMRVTDGLFASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVG
GSAKLKDMVDEIYKYM
>Mature_335_residues
TFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDVIEKADMILKGPVGETAMDV
VVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMR
RRKVTCVHKANVMRVTDGLFASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGLA
PSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVGG
SAKLKDMVDEIYKYM

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

Homologues:

Organism=Homo sapiens, GI5031777, Length=343, Percent_Identity=42.2740524781341, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=34.6938775510204, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI28178816, Length=342, Percent_Identity=34.5029239766082, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI28178821, Length=344, Percent_Identity=33.7209302325581, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=36.4485981308411, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI28178819, Length=210, Percent_Identity=35.7142857142857, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI87081683, Length=338, Percent_Identity=31.9526627218935, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1788101, Length=357, Percent_Identity=31.9327731092437, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787381, Length=359, Percent_Identity=30.6406685236769, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=337, Percent_Identity=38.8724035608309, Blast_Score=230, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=31.7784256559767, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17505779, Length=336, Percent_Identity=34.2261904761905, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25144293, Length=347, Percent_Identity=32.8530259365994, Blast_Score=164, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=38.135593220339, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=37.3913043478261, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=33.8235294117647, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=30.6406685236769, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=42.5219941348974, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=42.5219941348974, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=38.150289017341, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=32.7433628318584, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=32.7433628318584, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI20130355, Length=335, Percent_Identity=30.1492537313433, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=29.9120234604106, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=29.0322580645161, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=29.0322580645161, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=29.9120234604106, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI161078639, Length=339, Percent_Identity=29.4985250737463, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550436, Length=172, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_SULSO (Q9UXB2)

Other databases:

- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   F90220
- RefSeq:   NP_342231.1
- ProteinModelPortal:   Q9UXB2
- SMR:   Q9UXB2
- GeneID:   1454988
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0723
- NMPDR:   fig|273057.1.peg.654
- HOGENOM:   HBG518924
- OMA:   TCAHKAN
- PhylomeDB:   Q9UXB2
- ProtClustDB:   CLSK802842
- BioCyc:   SSOL273057:SSO0723-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- TIGRFAMs:   TIGR02088

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 37089; Mature: 36958

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 86-86 BINDING 96-96 BINDING 117-117 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTL
CEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHH
DVIEKADMILKGPVGETAMDVVVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENT
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEECCH
EDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMRRRKVTCVHKANVMRVTDGL
HHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEHHHHH
FASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKS
CCCCCCCCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
LENAIIETYKSGNKLTEDVGGSAKLKDMVDEIYKYM
HHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTL
EEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHH
DVIEKADMILKGPVGETAMDVVVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENT
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEEECCH
EDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMRRRKVTCVHKANVMRVTDGL
HHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEHHHHH
FASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCC
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKS
CCCCCCCCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
LENAIIETYKSGNKLTEDVGGSAKLKDMVDEIYKYM
HHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726