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The map label for this gene is ilvB-2 [H]

Identifier: 15897497

GI number: 15897497

Start: 507602

End: 509320

Strand: Reverse

Name: ilvB-2 [H]

Synonym: SSO0579

Alternate gene names: 15897497

Gene position: 509320-507602 (Counterclockwise)

Preceding gene: 15897498

Following gene: 15897496

Centisome position: 17.02

GC content: 40.84

Gene sequence:

>1719_bases
TTGCCAACAGGCGCACGCATTTTAGTTGATTCTCTAAAGAGAGAAGGAGTTAAGGTAGTATTCGGAATACCAGGATTATC
GAATATGCAGATATATGATGCTTTCGTAGAAGATTTGGCAAATGGTGAGCTTAGACATGTTCTAATGAGACATGAACAAG
CCGCAGCGCATGCTGCAGATGGATACGCAAGAGCTTCTGGAGTCCCAGGTGTATGCACAGCAACATCTGGTCCAGGTACG
ACCAATTTAACTACGGGGCTTATTACTGCATACTGGGATAGTTCTCCTGTTATTGCAATAACTGGTAACGTGCCTAGAAG
CGTTATGGGTAAGATGGCATTCCAAGAAGCTGATGCTATGGGAGTGTTCGAAAATGTGACCAAATACGTTATTGGTATTA
AGAGGATAGATGAGATACCCCAGTGGATTAAGAACGCATTTTATATAGCAACTACTGGAAGACCTGGGCCAGTTGTGGTT
GACATCCCTAGGGATATTTTCTATGAAAAAATGGAAGAGATAAAATGGCCAGAGAAACCACTTGTTAAGGGTTATAGGGA
TTTCCCAACTAGAATAGATCGTTTAGCACTGAAAAAGGCAGCTGAGATTCTAATCAATGCAGAGAGACCAATAATTTTGG
TAGGTACTGGAGTAGTATGGGCGAACGCGACTCCAGAAGTTTTGGAGTTAGCAGAACTATTGCATATTCCAATAGTCTCA
ACTTTTCCTGGAAAAACTGCAATACCGCATGATCATCCCTTATATTTCGGACCAATGGGATATTATGGAAGAGCTGAGGC
ATCAATGGCTGCTCTAGAATCTGATGCAATGCTAGTAGTAGGTGCGAGATTTAGCGATAGGACATTCACATCATATGACG
AAATGGTAGAGACCAGAAAGAAATTCATAATGGTTAATATAGATCCGACAGATGGAGAAAAGGCCATAAAAGTGGATGTA
GGACTCTACGGTAACGCTAAGATAATATTAAGGGAGCTAATAAAGGCAATAATAACACTGGGCCAAAAAAGAGATAAAAG
TGCATGGATAAAAAGAGTTAAAGAGTACAAGGAATATTATTCCCAATTTTACTACACTGAGGAGAACGGAAAATTAAAGC
CTTGGAAGATTATGAAAACAATTAGGCAATCATTACCAAGAGATGCTATAGTAACTACTGGTGTAGGTCAACATCAAATG
TGGGCAGAAGTGTTTTGGGAAGTATTGGAACCCAGAACTTTTCTAACATCATCTGGAATGGGTACAATGGGTTTTGGTCT
CCCTGCTGCAATGGGAGCCAAATTGGCTAGGCCTGATAAAATCGTAGTGGATCTAGATGGTGACGGTTCGTTCTTAATGA
CTGGAACAAATTTGGCCACGGCTGTAGATGAGCACATTCCAGTAATATCGGTGATATTTGATAATAGAACCTTAGGGTTA
GTGAGGCAAGTTCAAGATTTATTCTTCGGAAGGAGAATAGTAGGTGTAGATTATGGTCCTTCGCCAGACTTCGTTAAATT
GGCTGAAGCATTCGGTGCTTTAGGCTTTAATGCAACGACTTACGAGGAAATAGAAAAGTCGATTAAAAGTGCAATAAAGG
AAGACATCCCTGCAGTAATTAGAGTACCAGTAGATAAGGAAGAGCTGGCCTTACCTACGTTACCACCGGGTGGAAGATTA
AAACAGGTGATATTACGTGACCCAAGAAAGAGTAGTTAG

Upstream 100 bases:

>100_bases
AAATTTAATACATGTTTCAAAACTTTTAACGGGTTTCTTGATAAAGGAAATAAAAATTGGATTAGATACGAGAGTTCTAT
ATATAAGAAGGTGATATAAA

Downstream 100 bases:

>100_bases
GATATTAGCTTATTATAGGGACCCGGGATTAATAGAGAGGATAGCATCAAACTTTAGAAAACTATTTATGGATATTGAAT
GGATATACGGTTGGAAGGTA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MPTGARILVDSLKREGVKVVFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGT
TNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVV
DIPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDV
GLYGNAKIILRELIKAIITLGQKRDKSAWIKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQSLPRDAIVTTGVGQHQM
WAEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARPDKIVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
VRQVQDLFFGRRIVGVDYGPSPDFVKLAEAFGALGFNATTYEEIEKSIKSAIKEDIPAVIRVPVDKEELALPTLPPGGRL
KQVILRDPRKSS

Sequences:

>Translated_572_residues
MPTGARILVDSLKREGVKVVFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGT
TNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVV
DIPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDV
GLYGNAKIILRELIKAIITLGQKRDKSAWIKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQSLPRDAIVTTGVGQHQM
WAEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARPDKIVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
VRQVQDLFFGRRIVGVDYGPSPDFVKLAEAFGALGFNATTYEEIEKSIKSAIKEDIPAVIRVPVDKEELALPTLPPGGRL
KQVILRDPRKSS
>Mature_571_residues
PTGARILVDSLKREGVKVVFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAADGYARASGVPGVCTATSGPGTT
NLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAMGVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVVD
IPRDIFYEKMEEIKWPEKPLVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVST
FPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRKKFIMVNIDPTDGEKAIKVDVG
LYGNAKIILRELIKAIITLGQKRDKSAWIKRVKEYKEYYSQFYYTEENGKLKPWKIMKTIRQSLPRDAIVTTGVGQHQMW
AEVFWEVLEPRTFLTSSGMGTMGFGLPAAMGAKLARPDKIVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGLV
RQVQDLFFGRRIVGVDYGPSPDFVKLAEAFGALGFNATTYEEIEKSIKSAIKEDIPAVIRVPVDKEELALPTLPPGGRLK
QVILRDPRKSS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=26.3250883392226, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI21361361, Length=589, Percent_Identity=23.5993208828523, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=582, Percent_Identity=36.7697594501718, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790104, Length=555, Percent_Identity=35.6756756756757, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=31.2159709618875, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=27.8659611992945, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=24.8239436619718, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=26.9299820466786, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=26.9299820466786, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=590, Percent_Identity=26.1016949152542, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=35.958904109589, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6320816, Length=566, Percent_Identity=23.4982332155477, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=498, Percent_Identity=24.6987951807229, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=318, Percent_Identity=23.2704402515723, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=26.1324041811847, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63213; Mature: 63082

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGARILVDSLKREGVKVVFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAAD
CCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
GYARASGVPGVCTATSGPGTTNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAM
CHHHHCCCCCEEECCCCCCCCCCHHCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
GVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVVDIPRDIFYEKMEEIKWPEKP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHCCCCCCH
LVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCEE
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
KFIMVNIDPTDGEKAIKVDVGLYGNAKIILRELIKAIITLGQKRDKSAWIKRVKEYKEYY
CEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SQFYYTEENGKLKPWKIMKTIRQSLPRDAIVTTGVGQHQMWAEVFWEVLEPRTFLTSSGM
HHEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEECCCC
GTMGFGLPAAMGAKLARPDKIVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
CCCCCCCHHHHCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHCCCEEEEEECCCHHHH
VRQVQDLFFGRRIVGVDYGPSPDFVKLAEAFGALGFNATTYEEIEKSIKSAIKEDIPAVI
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEE
RVPVDKEELALPTLPPGGRLKQVILRDPRKSS
ECCCCHHHCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTGARILVDSLKREGVKVVFGIPGLSNMQIYDAFVEDLANGELRHVLMRHEQAAAHAAD
CCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
GYARASGVPGVCTATSGPGTTNLTTGLITAYWDSSPVIAITGNVPRSVMGKMAFQEADAM
CHHHHCCCCCEEECCCCCCCCCCHHCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
GVFENVTKYVIGIKRIDEIPQWIKNAFYIATTGRPGPVVVDIPRDIFYEKMEEIKWPEKP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHCCCCCCH
LVKGYRDFPTRIDRLALKKAAEILINAERPIILVGTGVVWANATPEVLELAELLHIPIVS
HHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEECCCHHHHHHHHHHHCCCEE
TFPGKTAIPHDHPLYFGPMGYYGRAEASMAALESDAMLVVGARFSDRTFTSYDEMVETRK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
KFIMVNIDPTDGEKAIKVDVGLYGNAKIILRELIKAIITLGQKRDKSAWIKRVKEYKEYY
CEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SQFYYTEENGKLKPWKIMKTIRQSLPRDAIVTTGVGQHQMWAEVFWEVLEPRTFLTSSGM
HHEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEECCCC
GTMGFGLPAAMGAKLARPDKIVVDLDGDGSFLMTGTNLATAVDEHIPVISVIFDNRTLGL
CCCCCCCHHHHCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHCCCEEEEEECCCHHHH
VRQVQDLFFGRRIVGVDYGPSPDFVKLAEAFGALGFNATTYEEIEKSIKSAIKEDIPAVI
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEE
RVPVDKEELALPTLPPGGRLKQVILRDPRKSS
ECCCCHHHCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]