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The map label for this gene is speB-1 [C]

Identifier: 15897374

GI number: 15897374

Start: 384378

End: 385271

Strand: Direct

Name: speB-1 [C]

Synonym: SSO0445

Alternate gene names: 15897374

Gene position: 384378-385271 (Clockwise)

Preceding gene: 15897367

Following gene: 15897376

Centisome position: 12.85

GC content: 36.35

Gene sequence:

>894_bases
GTGAGCGATGGAAGATTACTTTATTTAAATGAGAATAGTAGGAAATTTGCTGGATTTAACAAGGAAAAATCCCCATTTGT
AATAATTGGAATACCTATGGACATTACGAGTAGTTATAGGCCTGGAAGTAGATTTGCTCCGTCTTCAATTAGAGAATCGG
CTCAATATATTGAATTTTATTCAATACGAAATGATGTAGATATGGGGGAAATAGGTTTCAATGATGTAGGTGATATAATT
CTACATCCTTCAAACGTTGAGGAGAATTTAAGTAGAATATCCAGTGTGATTAGTTATTTCCAAGAAAGTGGGAAGATCAC
TATTTCTATAGGAGGAGAGCATACAATAACCGCGGGAATAATAAAGGGGACTAAGAGAGAAGGATTATGCCTAGTTAGCT
TCGATGCACATTTAGATTTAAGAGACGAGTATATGGGATATAGATATGATCACGCTTGTGTTATGAGGAGAATATCTGAA
TATGGTGTGAAAATTATTGAAGTCGGTACTAGGGCAGTTAGTAAAGAGGAAATAGAATATGCTAAACAAAAGGGTATACT
CTTCTTCACCCCTCAGCAAATTAGACTTTTAGGAGTCAAGGAAACTGCAAGAAGAATAATTACCTCAACGCAAGAATGTA
AATCGCTCTATATCTCAGTTGATATGGATGGGATAGATCCAGCTTATGCACCCGGCGTAGCAACACCAGAACCAGATGGA
TTAGACCCCACTAACCTGTTAGATATTATTAACTTAATTGCAGATAAAAGAGTTATAGGGTTTGATATTGTGGAAGTTTC
TCCTTCTTATGACACCTCAGGAATAACTAGTGTATTGGCTTCCAGAATAATTCTGGAGACAGCAGCTACAGTTTATAAAG
CTAGATCGCTTTAA

Upstream 100 bases:

>100_bases
CCCTCTTCTTTTAGCTAAGTCCAACACTTTATCTATTACTTCACTCACAACTCTCTAACATTACAACAAGTTTATAAAGT
TCTATGACCAAGATTAACGA

Downstream 100 bases:

>100_bases
CTATTTTTATAGTAGGCTTTCTAACCATAAATATTATTTTGTAACCGCAGTATGGACATCTTACACCTGGCAATACTTTT
AATTGTTCATCTGTAAACGT

Product: agmatinase SpeB

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFYSIRNDVDMGEIGFNDVGDII
LHPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGIIKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISE
YGVKIIEVGTRAVSKEEIEYAKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDG
LDPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL

Sequences:

>Translated_297_residues
MSDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFYSIRNDVDMGEIGFNDVGDII
LHPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGIIKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISE
YGVKIIEVGTRAVSKEEIEYAKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDG
LDPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL
>Mature_296_residues
SDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFYSIRNDVDMGEIGFNDVGDIIL
HPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGIIKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISEY
GVKIIEVGTRAVSKEEIEYAKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDGL
DPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=280, Percent_Identity=31.4285714285714, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1789306, Length=259, Percent_Identity=26.6409266409266, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32973; Mature: 32842

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFY
CCCCEEEEEECCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHE
SIRNDVDMGEIGFNDVGDIILHPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGI
ECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEE
IKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISEYGVKIIEVGTRAVSKEEIEY
EECCCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHH
AKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDG
HHHCCEEEECCCCEEEEEHHHHHHHHHCCHHHHCEEEEEEECCCCCCCCCCCCCCCCCCC
LDPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL
CCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFY
CCCEEEEEECCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHE
SIRNDVDMGEIGFNDVGDIILHPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGI
ECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEE
IKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISEYGVKIIEVGTRAVSKEEIEY
EECCCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHHHHHH
AKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDG
HHHCCEEEECCCCEEEEEHHHHHHHHHCCHHHHCEEEEEEECCCCCCCCCCCCCCCCCCC
LDPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL
CCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]