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The map label for this gene is endA

Identifier: 15897369

GI number: 15897369

Start: 380629

End: 381177

Strand: Reverse

Name: endA

Synonym: SSO0439

Alternate gene names: 15897369

Gene position: 381177-380629 (Counterclockwise)

Preceding gene: 15897370

Following gene: 15897368

Centisome position: 12.74

GC content: 33.52

Gene sequence:

>549_bases
ATGGTAAAAGCTTTATTGGTAGGATCTAAAGTACTAGTTCCAAGTATAGATGAGTCAAGATACCTTTATTCAAATGGTTT
CTATGGAAAACCCATAGGAATTTCTAAGCCAAAAGGCCCTAAAGATATAGTTAGACCTCTGGAATTGTCACTAATTGAAA
GTGTATATCTAACTAAGAAGGGTTTAATTAATGTTGTGGATAAAAATGGCGACCTTCTTGAGTATAAGAAATTATATGAA
TATAGTGCAATGAAGATTAATAAATTCGAGATCTTATACAAAGTTTATGAAGACTTAAGGGAAAAAGGATTTATAGTTAG
ATCTGGAGTAAAGTATGGAGCGGACTTTGCGGTATATACATTAGGGCCAGGATTAGAACATGCACCCTATGTCGTTATTG
CTGTTGATATTGATGAGGAAATCACACCTCATGAACTATTGAGTTTCGGTAGAGTCTCACATAGCACTAAAAAGAGATTA
GTATTGGCTCTAGTGGATCGTAAAAGTGAAGGTATAAGATACATAATGTTTAAATGGGTCAAAATGTAA

Upstream 100 bases:

>100_bases
AATGTTACTTAAAGATATAGCTGACAGATTAGAAGATAGTGAGGATCTATTGAAGAGTTCTGCTGACAATATAACCTATA
TCGCATATGAGAGGATGTAG

Downstream 100 bases:

>100_bases
GTTTAATATGATTGTCTAAAAGTTTGGGGGTATAAGTAGTTTGAGGAACGGAAATAGAGGCTTTGAAAGACAGCCCTCCT
TTACTGATGAGCAGAAACCA

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKKGLINVVDKNGDLLEYKKLYE
YSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRL
VLALVDRKSEGIRYIMFKWVKM

Sequences:

>Translated_182_residues
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKKGLINVVDKNGDLLEYKKLYE
YSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRL
VLALVDRKSEGIRYIMFKWVKM
>Mature_182_residues
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKKGLINVVDKNGDLLEYKKLYE
YSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRL
VLALVDRKSEGIRYIMFKWVKM

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6323134, Length=123, Percent_Identity=34.1463414634146, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_SULSO (Q97ZY3)

Other databases:

- EMBL:   AE006641
- PIR:   E90188
- RefSeq:   NP_341974.1
- ProteinModelPortal:   Q97ZY3
- SMR:   Q97ZY3
- GeneID:   1455574
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0439
- NMPDR:   fig|273057.1.peg.397
- HOGENOM:   HBG297289
- OMA:   DFRLYER
- ProtClustDB:   PRK09297
- BioCyc:   SSOL273057:SSO0439-MONOMER
- BRENDA:   3.1.27.9
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 20711; Mature: 20711

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: ACT_SITE 119-119 ACT_SITE 127-127 ACT_SITE 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKK
CCEEEEECCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYT
CEEEEECCCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
LGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRLVLALVDRKSEGIRYIMFKWV
ECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHEEEEEEECCCCCCCEEEEEEEE
KM
CC
>Mature Secondary Structure
MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKK
CCEEEEECCEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYT
CEEEEECCCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
LGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRLVLALVDRKSEGIRYIMFKWV
ECCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHEEEEEEECCCCCCCEEEEEEEE
KM
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726